FastQCFastQC Report
Wed 25 May 2016
SRR1779863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568062
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT1150.042.60199722
ACGATAC801.4242687E-939.36881324
ACCGTTC459.20454E-538.8827748
TCGGTGG1450.038.6112245
ATCTCGG2200.038.17245542
CGGTGGT1750.037.99736846
CTCGGTG1500.037.3241844
GATCTCG2200.036.58193641
TCGCCGT1500.035.00374252
CGATACT601.2167746E-534.994525
AGATCTC3250.034.4530940
ACGAATA957.6415745E-933.1526824
GTAGATC2600.032.30545838
TAGATCT3050.032.12609539
TCGTTCG552.9943226E-431.81318323
CGTTCGG552.9943226E-431.81318324
TTCGGTA552.9943226E-431.81318326
TCTCGGT2250.031.10348343
ACTGACG1052.0258085E-829.99528511
TCGTTGT604.9729075E-429.17492334