FastQCFastQC Report
Wed 25 May 2016
SRR1779863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568062
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT327065.757470135302133TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG42230.7434047691977285TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC23210.4085821618062817RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT7870.13854121557153973TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6480.11407205551506702RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC49050.052.61745548
GCGTATG200.006937900652.52358646
TATGCCG49500.052.5173243
TCGTATG49900.052.376840
CTCGTAT48900.052.37465339
CGTATGC49900.052.30668641
CCGTCTT49300.052.27499847
ATGCCGT49700.052.16518844
GCCGTCT49350.052.1510846
TCTCGTA47050.052.05438638
TGCCGTC49800.052.0176545
ATCTCGT47150.051.79557437
GTATGCC50500.051.4080942
GATCTCG47450.051.0256836
GTCTTCT51050.050.8349149
CTTGAAA50150.050.56447657
GCTTGAA50600.050.46533656
CTGCTTG51700.050.3401454
TCTTCTG51650.050.11318650
TGCTTGA51400.049.95247755
Produced by FastQC (version 0.11.5)