##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779863_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568062
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.562919188398446	34.0	34.0	34.0	34.0	34.0
2	33.65940337498372	34.0	34.0	34.0	34.0	34.0
3	33.76781055589002	34.0	34.0	34.0	34.0	34.0
4	33.585328714119235	34.0	34.0	34.0	34.0	34.0
5	33.635932345413	34.0	34.0	34.0	34.0	34.0
6	37.321528283884504	38.0	38.0	38.0	37.0	38.0
7	37.366363178667115	38.0	38.0	38.0	37.0	38.0
8	37.2737623710088	38.0	38.0	38.0	36.0	38.0
9	37.189539170020176	38.0	38.0	38.0	36.0	38.0
10-11	37.14949512553207	38.0	38.0	38.0	36.0	38.0
12-13	37.29958437635328	38.0	38.0	38.0	36.5	38.0
14-15	37.30876383211692	38.0	38.0	38.0	36.5	38.0
16-17	37.253843770574335	38.0	38.0	38.0	36.0	38.0
18-19	37.138303565455885	38.0	38.0	38.0	36.0	38.0
20-21	37.13860458893571	38.0	38.0	38.0	36.0	38.0
22-23	37.11948871778081	38.0	38.0	38.0	36.0	38.0
24-25	37.28792279715947	38.0	38.0	38.0	36.5	38.0
26-27	37.21390886910231	38.0	38.0	38.0	36.0	38.0
28-29	36.7854292313163	38.0	37.0	38.0	35.0	38.0
30-31	37.03015251856311	38.0	38.0	38.0	36.0	38.0
32-33	37.06899422950312	38.0	38.0	38.0	36.0	38.0
34-35	37.1373494442508	38.0	38.0	38.0	36.0	38.0
36-37	37.1524085399129	38.0	38.0	38.0	36.0	38.0
38-39	36.76480120127732	38.0	38.0	38.0	34.5	38.0
40-41	36.95024310726646	38.0	37.5	38.0	35.5	38.0
42-43	37.203597494639666	38.0	38.0	38.0	36.5	38.0
44-45	36.994712725019454	38.0	38.0	38.0	35.5	38.0
46-47	37.12230355137291	38.0	38.0	38.0	36.0	38.0
48-49	36.8085349838574	38.0	37.0	38.0	35.0	38.0
50-51	36.98400526703071	38.0	37.5	38.0	35.5	38.0
52-53	36.9049892441318	38.0	38.0	38.0	35.5	38.0
54-55	36.8940828641944	38.0	37.5	38.0	35.5	38.0
56-57	36.77729543606156	38.0	37.0	38.0	35.0	38.0
58-59	36.87136967443695	38.0	37.5	38.0	35.5	38.0
60-61	36.60339452383718	38.0	37.0	38.0	34.0	38.0
62-63	36.41573102935948	38.0	37.0	38.0	34.0	38.0
64-65	36.73373416986174	38.0	37.0	38.0	34.5	38.0
66-67	36.53936982230813	38.0	37.0	38.0	34.5	38.0
68-69	36.65440216032757	38.0	37.0	38.0	34.0	38.0
70-71	36.21120317852629	38.0	37.0	38.0	32.5	38.0
72-73	35.41406571817865	38.0	37.0	38.0	27.5	38.0
74-75	35.2977588713908	38.0	37.0	38.0	26.0	38.0
76	35.00227615999662	38.0	37.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	1.0
21	7.0
22	20.0
23	56.0
24	126.0
25	266.0
26	508.0
27	911.0
28	1567.0
29	2707.0
30	4215.0
31	6907.0
32	10465.0
33	16719.0
34	28359.0
35	52251.0
36	85125.0
37	357849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.8508455829256	18.053309943745223	11.568335288336556	31.52750918499262
2	18.911139981199234	19.385207952653055	37.66701522016963	24.03663684597808
3	18.589168083765504	21.007037964165885	34.202428608144885	26.201365343923726
4	13.421246272413926	22.178388978667822	34.80218708521253	29.598177663705723
5	13.56718104713922	32.77599980283842	38.9871176033602	14.669701546662159
6	28.370494769937082	40.26303466875095	17.558470730307608	13.807999831004361
7	26.12021223035514	28.693522890107065	20.873073713784763	24.313191165753032
8	23.38459534346603	39.256982512472234	18.617157986276144	18.741264157785594
9	23.140607891392136	16.603117265368923	19.76967302864828	40.486601814590664
10-11	24.028627156894846	27.19562301298098	27.7250546595266	21.050695170597574
12-13	23.757177913678436	21.613837926141866	28.070263457157846	26.558720703021855
14-15	21.604683995761025	22.389897581602007	22.545778453760327	33.45963996887664
16-17	25.44889466290651	28.218222658794286	23.19368308388873	23.13919959441047
18-19	25.363340621270215	25.331477902059984	24.397072854723604	24.908108621946194
20-21	21.57158901669184	24.46335083142333	24.359136854779937	29.60592329710489
22-23	21.811703652066146	28.145783382799767	24.075981142903416	25.96653182223067
24-25	25.36772310500804	28.199483678120046	24.03026811593565	22.402525100936263
26-27	25.084497818900047	28.69149846319592	24.411947991592466	21.812055726311563
28-29	21.463590241910214	28.601455474930553	24.791924120958626	25.143030162200603
30-31	21.394671708369863	28.47849354471871	24.71790051085973	25.4089342360517
32-33	20.97271072523774	32.384845316180275	24.708570543356185	21.9338734152258
34-35	25.08176924349807	25.3422161665452	28.187680218004374	21.388334371952357
36-37	25.453559646658285	27.34947945822815	25.499593354246542	21.69736754086702
38-39	21.347933852290772	25.056859990634827	28.54758811538177	25.047618041692633
40-41	21.173657100809418	23.483352169305462	29.44036038319761	25.90263034668751
42-43	24.6574739595711	23.299604442496292	29.140018906486826	22.902902691445785
44-45	20.621868739679826	26.82198422003232	30.014329421788467	22.541817618499387
46-47	23.948002154694382	22.951896095848692	27.38301453010411	25.71708721935282
48-49	23.151258137315995	22.820660420869554	27.110421045590094	26.917660396224356
50-51	20.001320503904292	23.418256406644776	30.681380014613573	25.89904307483736
52-53	18.83079719013766	23.66476067038004	34.242247608112606	23.262194531369694
54-55	18.522560036356044	23.868519345415027	30.941478919570176	26.667441698658756
56-57	22.095661348820087	23.62707433888017	27.100408482484927	27.176855829814816
58-59	18.67868671730398	23.538336365349426	33.315487585170885	24.467489332175706
60-61	22.04123359127075	26.82208658103191	27.02918790218337	24.107491925513965
62-63	18.65367411915529	31.62984446980263	25.13955806319823	24.57692334784385
64-65	18.863768616082393	32.33240573881979	23.94549582582469	24.85832981927313
66-67	18.71811828188964	32.24939527650855	23.508444114919918	25.52404232668189
68-69	18.76508020623076	32.533985206103125	23.33159332364444	25.369341264021678
70-71	18.92112208373901	31.980759852036783	23.2185520943556	25.879565969868608
72-73	19.218280904712852	31.00948205277198	22.932569134465545	26.83966790804962
74-75	19.550049034020983	30.96529236424971	22.654862114924477	26.82979648680483
76	19.378982377621394	31.406709145361294	22.27508094603422	26.939227530983096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	10.5
12	18.0
13	13.0
14	21.0
15	55.5
16	109.5
17	142.0
18	161.0
19	277.5
20	465.0
21	555.0
22	806.0
23	1120.5
24	1526.5
25	1869.0
26	2302.0
27	3156.0
28	4129.5
29	4682.0
30	5350.5
31	6912.0
32	9040.0
33	10275.0
34	11509.0
35	13821.5
36	15922.5
37	16945.0
38	18594.0
39	26983.5
40	37137.0
41	39620.0
42	38690.0
43	36437.0
44	33238.5
45	31309.5
46	30326.0
47	29984.0
48	28858.0
49	26571.5
50	25069.0
51	23976.0
52	21356.5
53	18580.0
54	17330.0
55	16734.5
56	15022.5
57	12725.0
58	11544.0
59	10569.0
60	8742.0
61	7368.0
62	6846.0
63	6375.5
64	5567.0
65	4961.0
66	4372.0
67	4051.0
68	3811.0
69	3386.5
70	3130.5
71	3059.0
72	2757.0
73	2336.5
74	2087.0
75	1956.0
76	1813.0
77	1580.0
78	1363.5
79	1237.0
80	1104.0
81	845.5
82	628.5
83	537.0
84	482.0
85	346.0
86	237.5
87	210.0
88	151.5
89	77.0
90	61.5
91	40.5
92	19.0
93	13.5
94	5.5
95	6.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050698691340029786
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.682041748964022E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.281113681253102E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.017075600902718365
52-53	0.03608761015522954
54-55	0.060468751650348034
56-57	0.0617890300706613
58-59	0.04101664959106577
60-61	0.03943231548668984
62-63	0.03485535029627048
64-65	0.018571916445740078
66-67	0.04233692801137904
68-69	0.010122134555735114
70-71	0.01461108118480025
72-73	0.025877457038140202
74-75	0.01566730392105087
76	0.016019378166467743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	568062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.687427162671785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	29.714551233632985	6.741467167845275
2	13.194207062243057	5.986852233876981
3	12.91958284046218	8.7933628399527
4	12.050847101921052	10.936108634933131
5	9.91607152474636	11.24850752287634
6	7.219370611850135	9.827336695000989
7	4.908619021606606	7.795475556540358
8	3.0753785944714522	5.58179422877688
9	1.8950593998339025	3.8694619892401354
>10	4.915750765352152	18.714229137061064
>50	0.16546728007698974	2.413430067187179
>100	0.019605127971207316	0.6629148861289662
>500	0.00313682047539317	0.4458574086524931
>1k	0.001568410237696585	1.1642820759065902
>5k	0.0	0.0
>10k+	7.842051188482925E-4	5.818919556020926
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	32706	5.757470135302133	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	4223	0.7434047691977285	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	2321	0.4085821618062817	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	787	0.13854121557153973	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	648	0.11407205551506702	RNA PCR Primer, Index 5 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015843341043759308	0.0
2	0.0	0.0	0.0	0.0017603712270843675	0.0
3	0.0	0.0	0.0	0.0017603712270843675	0.0
4	0.0	0.0	0.0	0.004048853822294045	0.0
5	0.0	0.0	0.0	0.006337336417503723	0.0
6	0.0	0.0	0.0	0.006337336417503723	0.0
7	0.0	0.0	0.0	0.006689410662920597	0.0
8	0.0	0.0	0.0	0.007217522031045907	0.0
9	0.0	0.0	0.0	0.008625819012713401	0.0
10	0.0	0.0	0.0	0.011266375853339953	0.0
11	0.0	0.0	0.0	0.011794487221465262	0.0
12	0.0	0.0	0.0	0.012146561466882136	0.0
13	0.0	0.0	0.0	0.013730895571258067	0.0
14	0.0	0.0	0.0	0.013730895571258067	0.0
15	0.0	0.0	0.0	0.01408296981667494	0.0
16	0.0	0.0	0.0	0.014611081184800251	0.0
17	0.0	0.0	0.0	0.014787118307508687	0.0
18	0.0	0.0	0.0	0.015315229675633998	0.0
19	0.0	0.0	0.0	0.016899563780009928	0.0
20	0.0	0.0	0.0	0.01830786076167742	0.0
21	0.0	0.0	0.0	0.020420306234178665	0.0
22	0.0	0.0	0.0	0.023060863074805215	0.0
23	0.0	0.0	0.0	0.026229531283557077	0.0
24	0.0	0.0	0.0	0.03133460784210174	0.0
25	0.0	0.0	0.0	0.03362309043731142	0.0
26	0.0	0.0	0.0	0.038024018505022336	0.0
27	0.0	0.0	0.0	0.06618995813837222	0.0
28	0.0	0.0	0.0	0.09699645461234865	0.0
29	0.0	0.0	0.0	0.12375409726403104	0.0
30	0.0	0.0	0.0	0.16019378166467746	0.0
31	0.0	0.0	0.0	0.20825191616408067	0.0
32	0.0	0.0	0.0	0.25930268174952736	0.0
33	0.0	0.0	0.0	0.31739493224331145	0.0
34	0.0	0.0	0.0	0.3846411131179343	0.0
35	0.0	0.0	0.0	0.5062827649094641	0.0
36	0.0	0.0	0.0	0.7124222356010436	0.0
37	0.0	0.0	0.0	1.084212638761262	0.0
38	0.0	0.0	0.0	1.566730392105087	0.0
39	0.0	0.0	0.0	2.128288813545	0.0
40	0.0	0.0	0.0	2.8014547707820627	0.0
41	0.0	0.0	0.0	3.6497776651140192	0.0
42	0.0	0.0	0.0	4.643859297048562	0.0
43	0.0	0.0	0.0	5.660825754935201	0.0
44	0.0	0.0	0.0	6.55051737310364	0.0
45	0.0	0.0	0.0	7.537205445884428	0.0
46	0.0	0.0	0.0	8.501360766958536	0.0
47	0.0	0.0	0.0	9.34334632487299	0.0
48	0.0	0.0	0.0	10.130232263379702	0.0
49	0.0	0.0	0.0	10.832972457231781	0.0
50	0.0	0.0	0.0	11.568983667275756	0.0
51	0.0	0.0	0.0	12.361854867954554	0.0
52	0.0	0.0	0.0	13.245385186828198	0.0
53	0.0	0.0	0.0	14.117297055603085	0.0
54	0.0	0.0	0.0	14.954705648327119	0.0
55	0.0	0.0	0.0	15.690892895493802	0.0
56	0.0	0.0	0.0	16.414581506948185	0.0
57	0.0	0.0	0.0	17.080001830786077	0.0
58	0.0	0.0	0.0	17.719192623340412	0.0
59	0.0	0.0	0.0	18.315254320831176	0.0
60	0.0	0.0	0.0	18.876460668025672	0.0
61	0.0	0.0	0.0	19.439427386447253	0.0
62	0.0	0.0	0.0	20.1101288239664	0.0
63	0.0	0.0	0.0	20.748087356661774	0.0
64	0.0	0.0	0.0	21.40259337889174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	4905	0.0	52.617455	48
GCGTATG	20	0.0069379006	52.523586	46
TATGCCG	4950	0.0	52.51732	43
TCGTATG	4990	0.0	52.3768	40
CTCGTAT	4890	0.0	52.374653	39
CGTATGC	4990	0.0	52.306686	41
CCGTCTT	4930	0.0	52.274998	47
ATGCCGT	4970	0.0	52.165188	44
GCCGTCT	4935	0.0	52.15108	46
TCTCGTA	4705	0.0	52.054386	38
TGCCGTC	4980	0.0	52.01765	45
ATCTCGT	4715	0.0	51.795574	37
GTATGCC	5050	0.0	51.40809	42
GATCTCG	4745	0.0	51.02568	36
GTCTTCT	5105	0.0	50.83491	49
CTTGAAA	5015	0.0	50.564476	57
GCTTGAA	5060	0.0	50.465336	56
CTGCTTG	5170	0.0	50.34014	54
TCTTCTG	5165	0.0	50.113186	50
TGCTTGA	5140	0.0	49.952477	55
>>END_MODULE