##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779862_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84075
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.189616413916145	27.0	12.0	34.0	12.0	34.0
2	25.438382396669642	31.0	12.0	34.0	12.0	34.0
3	25.352566161165626	31.0	11.0	34.0	11.0	34.0
4	26.006743978590546	32.0	21.0	34.0	12.0	34.0
5	25.15110318168302	32.0	11.0	34.0	11.0	34.0
6	28.089253642581028	34.0	21.0	38.0	11.0	38.0
7	28.283520666071958	34.0	21.0	38.0	12.0	38.0
8	27.947915551590842	34.0	21.0	38.0	11.0	38.0
9	28.376616116562595	34.0	21.0	38.0	11.0	38.0
10-11	27.283788284269995	32.0	11.5	38.0	11.0	38.0
12-13	27.668581623550402	34.0	16.0	38.0	11.0	38.0
14-15	26.811239964317572	29.5	11.0	37.5	11.0	38.0
16-17	27.084388938447816	32.5	11.0	38.0	11.0	38.0
18-19	27.440564971751414	34.0	11.0	38.0	11.0	38.0
20-21	26.907665774606006	30.5	16.0	38.0	11.0	38.0
22-23	27.40885518881951	32.5	11.0	38.0	11.0	38.0
24-25	27.663728813559324	34.0	16.0	38.0	11.0	38.0
26-27	26.77391614629795	31.0	11.0	38.0	11.0	38.0
28-29	27.329360689860245	33.5	11.0	38.0	11.0	38.0
30-31	27.581671126969965	34.0	11.0	38.0	11.0	38.0
32-33	27.782384775498066	34.0	11.0	38.0	11.0	38.0
34-35	27.78841510556051	34.0	11.0	38.0	11.0	38.0
36-37	27.431727624145108	33.5	11.0	38.0	11.0	38.0
38-39	27.32337793636634	33.5	11.0	38.0	11.0	38.0
40-41	27.31859649122807	33.5	11.0	38.0	11.0	38.0
42-43	27.601326196848053	34.0	11.0	38.0	11.0	38.0
44-45	27.665322628605413	34.0	11.0	38.0	11.0	38.0
46-47	27.54271186440678	34.0	11.0	38.0	11.0	38.0
48-49	27.25845970859352	33.5	11.0	38.0	11.0	38.0
50-51	26.871097234611952	32.5	11.0	38.0	11.0	38.0
52-53	27.3302111210229	33.5	11.0	38.0	11.0	38.0
54-55	27.359435028248587	33.5	11.0	38.0	11.0	38.0
56-57	27.41766874814154	34.0	11.0	38.0	11.0	38.0
58-59	27.383990484686294	34.0	11.0	38.0	11.0	38.0
60-61	27.470217068093962	34.0	11.0	38.0	11.0	38.0
62-63	27.337329765090693	34.0	11.0	38.0	11.0	38.0
64-65	27.315194766577463	33.5	11.0	38.0	11.0	38.0
66-67	27.469824561403506	33.5	11.0	38.0	11.0	38.0
68-69	27.08470413321439	31.5	11.0	38.0	11.0	38.0
70-71	27.09941123996432	32.0	11.0	38.0	11.0	38.0
72-73	27.201278620279513	32.0	11.0	38.0	11.0	38.0
74-75	27.311650312221232	32.5	11.0	38.0	11.0	38.0
76	26.355515908415107	31.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	58.0
13	655.0
14	2076.0
15	3728.0
16	4926.0
17	5786.0
18	5965.0
19	5108.0
20	4016.0
21	2757.0
22	2001.0
23	1402.0
24	1077.0
25	989.0
26	931.0
27	943.0
28	980.0
29	1084.0
30	1274.0
31	1486.0
32	1913.0
33	2473.0
34	3245.0
35	4938.0
36	8272.0
37	15990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.110949877488878	20.285700692247303	16.585389062016795	35.01796036824702
2	21.571216176033303	21.099018733273862	32.504311626523936	24.8254534641689
3	14.510853404698187	28.40321141837645	32.32827832292596	24.757656853999404
4	11.664585191793043	29.215581326196848	24.23312518584597	34.88670829616414
5	19.787094855783526	30.88670829616414	29.721082366934287	19.60511448111805
6	21.988962104812426	44.058805338154485	13.34181792230654	20.610414634726553
7	19.90769377170862	35.26906789741638	15.378027311224246	29.445211019650756
8	17.14384270438082	45.95877294191814	14.414007208193075	22.483377145507962
9	20.37303579287949	22.3309978945365	15.601841388416382	41.69412492416763
10-11	19.0090459792674	31.97732855163226	21.38502804194099	27.628597427159345
12-13	18.73446327683616	24.87481415402914	24.24382991376747	32.14689265536723
14-15	25.789473684210527	27.574189711567055	19.009812667261375	27.626523936961046
16-17	27.085501549221224	26.029295446300605	18.706623292437065	28.178579712041106
18-19	20.588899990484347	25.445451517746694	23.141473974688363	30.8241745170806
20-21	25.11448111804936	24.64942016057092	20.458519179304194	29.77757954207553
22-23	27.330577775134557	24.325433405691516	18.76654078325255	29.57744803592138
24-25	20.295931536096393	30.626178284477273	18.73836583465658	30.339524344769753
26-27	23.951066626225863	30.60833675296021	19.9075808667416	25.53301575407233
28-29	21.18829546806233	28.827167836326872	23.01237064351136	26.97216605209944
30-31	22.07433838834374	27.35831103181683	22.95153137079988	27.61581920903955
32-33	19.321439191198333	29.559916741005054	22.840915848944395	28.277728218852218
34-35	18.845751260824056	31.73351413074508	22.10367304215434	27.317061566276525
36-37	23.722905612791166	25.75602560137048	22.8366128149611	27.684455970877252
38-39	24.2064129329265	23.92983958745963	24.896954124511534	26.966793355102332
40-41	20.52299165021291	28.411875252753525	23.158764897590217	27.90636819944335
42-43	17.098525712314675	27.40605058608734	24.836900606010147	30.658523095587842
44-45	16.801574887444318	24.488667114708665	28.136839162835507	30.57291883501151
46-47	19.054359462352803	26.825859402878553	25.007136909718092	29.112644225050555
48-49	19.738923580136785	27.625334522747547	23.892358013678265	28.743383883437406
50-51	24.914509328147393	26.855667957204115	20.698555430664836	27.531267283983656
52-53	15.395276077633637	30.091064280318573	23.52801222914176	30.985647412906026
54-55	17.84182760076844	26.28633284364164	23.267294344358337	32.60454521123158
56-57	19.685479601972293	28.048700700057694	21.493020240410637	30.772799457559373
58-59	18.351930717803526	28.217863005876616	22.411434417453783	31.018771858866074
60-61	16.064980875730917	26.340334659837843	23.496975212805822	34.09770925162541
62-63	18.672122664795133	26.79723790094746	22.269274919260347	32.261364514997055
64-65	18.988930459971805	26.148145063913063	21.648951040631932	33.213973435483204
66-67	16.875747247452665	32.10799621692036	19.139051969759155	31.877204565867817
68-69	17.772332871441403	33.95797774592772	18.29270468453574	29.976984698095144
70-71	16.578351085657193	33.98098092740281	19.994409653457986	29.44625833348201
72-73	16.02038752958809	31.560228854182775	20.005114724459087	32.41426889177006
74-75	15.93590902602655	29.91566351049151	19.004258457439217	35.14416900604272
76	14.96473219064838	28.889866898216983	20.648023694258423	35.49737721687622
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	3.0
12	6.0
13	3.0
14	2.0
15	5.5
16	11.0
17	15.0
18	21.0
19	31.0
20	45.5
21	56.0
22	77.0
23	111.5
24	154.5
25	184.0
26	212.0
27	298.5
28	399.5
29	442.0
30	545.0
31	708.5
32	894.0
33	1019.0
34	1139.0
35	1430.5
36	1753.0
37	1904.0
38	2074.0
39	2511.5
40	2912.5
41	3242.5
42	3439.0
43	3721.0
44	4123.0
45	4365.5
46	4488.0
47	4614.0
48	4798.5
49	4759.5
50	4662.0
51	4610.5
52	4364.0
53	4030.5
54	3892.0
55	3698.5
56	3330.0
57	2945.5
58	2736.0
59	2595.5
60	2313.0
61	1914.5
62	1658.0
63	1526.5
64	1317.0
65	1165.0
66	968.0
67	845.0
68	758.5
69	609.5
70	513.5
71	480.0
72	431.5
73	360.0
74	325.0
75	313.0
76	271.0
77	221.0
78	196.0
79	179.0
80	158.5
81	118.5
82	99.5
83	100.0
84	76.5
85	48.5
86	37.5
87	31.0
88	27.0
89	22.0
90	14.5
91	9.5
92	11.0
93	9.5
94	7.5
95	4.5
96	2.0
97	2.5
98	3.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011894142134998514
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0011894142134998514
7	0.008325899494498959
8	0.004757656853999406
9	0.009515313707998812
10-11	0.0053523639607493305
12-13	0.0
14-15	0.0
16-17	5.947071067499257E-4
18-19	0.003568242640499554
20-21	0.0
22-23	0.0029735355337496285
24-25	0.001784121320249777
26-27	0.001784121320249777
28-29	0.005947071067499257
30-31	0.0
32-33	0.0
34-35	0.003568242640499554
36-37	0.020220041629497473
38-39	0.012488849241748439
40-41	0.0011894142134998514
42-43	5.947071067499257E-4
44-45	0.006541778174249183
46-47	0.005947071067499257
48-49	0.0
50-51	0.001784121320249777
52-53	0.016057091882247992
54-55	0.010110020814748736
56-57	0.012488849241748439
58-59	0.015462384775498066
60-61	0.0231935771632471
62-63	0.010110020814748736
64-65	0.018435920309247695
66-67	0.019625334522747548
68-69	5.947071067499257E-4
70-71	0.001784121320249777
72-73	0.004757656853999406
74-75	0.008325899494498959
76	0.004757656853999406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	84075.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.25245316681534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74006411663959	72.1034790365745
2	5.2230175808411285	8.487659827534939
3	2.952585891411591	7.197145405887601
4	1.572175135040183	5.109723461195361
5	0.8417138758362244	3.4195658638120725
6	0.3923118586506229	1.912578055307761
7	0.18005357691800974	1.024085637823372
8	0.04537935678421384	0.2949747249479631
9	0.026349303939220938	0.1926851025869759
>10	0.026349303939220938	0.25810288432946776
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004757656853999405	0.0
2	0.0	0.0	0.0	0.004757656853999405	0.0
3	0.0	0.0	0.0	0.004757656853999405	0.0
4	0.0	0.0	0.0	0.004757656853999405	0.0
5	0.0	0.0	0.0	0.005947071067499257	0.0
6	0.0	0.0	0.0	0.005947071067499257	0.0
7	0.0	0.0	0.0	0.005947071067499257	0.0
8	0.0	0.0	0.0	0.005947071067499257	0.0
9	0.0	0.0	0.0	0.005947071067499257	0.0
10	0.0	0.0	0.0	0.00951531370799881	0.0
11	0.0	0.0	0.0	0.00951531370799881	0.0
12	0.0	0.0	0.0	0.00951531370799881	0.0
13	0.0	0.0	0.0	0.013083556348498365	0.0
14	0.0	0.0	0.0	0.013083556348498365	0.0
15	0.0	0.0	0.0	0.013083556348498365	0.0
16	0.0	0.0	0.0	0.015462384775498068	0.0
17	0.0	0.0	0.0	0.015462384775498068	0.0
18	0.0	0.0	0.0	0.01903062741599762	0.0
19	0.0	0.0	0.0	0.028545941123996433	0.0
20	0.0	0.0	0.0	0.030924769550996135	0.0
21	0.0	0.0	0.0	0.032114183764495985	0.0
22	0.0	0.0	0.0	0.07612250966399048	0.0
23	0.0	0.0	0.0	0.07731192387749034	0.0
24	0.0	0.0	0.0	0.088016651798989	0.0
25	0.0	0.0	0.0	0.09396372286648826	0.0
26	0.0	0.0	0.0	0.09396372286648826	0.0
27	0.0	0.0	0.0	0.11537317870948557	0.0
28	0.0	0.0	0.0	0.15462384775498067	0.0
29	0.0	0.0	0.0	0.18673803151947665	0.0
30	0.0	0.0	0.0	0.21647338685697295	0.0
31	0.0	0.0	0.0	0.24977698483496877	0.0
32	0.0	0.0	0.0	0.28783823966696404	0.0
33	0.0	0.0	0.0	0.3294677371394588	0.0
34	0.0	0.0	0.0	0.407969075230449	0.0
35	0.0	0.0	0.0	0.4888492417484389	0.0
36	0.0	0.0	0.0	0.6732084448409158	0.0
37	0.0	0.0	0.0	0.9669937555753791	0.0
38	0.0	0.0	0.0	1.328575676479334	0.0
39	0.0	0.0	0.0	1.6984834968777878	0.0
40	0.0	0.0	0.0	2.143324412726732	0.0
41	0.0	0.0	0.0	2.6523936961046686	0.0
42	0.0	0.0	0.0	3.2256913470115967	0.0
43	0.0	0.0	0.0	3.7966101694915255	0.0
44	0.0	0.0	0.0	4.4793339280404405	0.0
45	0.0	0.0	0.0	5.016949152542373	0.0
46	0.0	0.0	0.0	5.625929229854297	0.0
47	0.0	0.0	0.0	6.069580731489741	0.0
48	0.0	0.0	0.0	6.570324115373179	0.0
49	0.0	0.0	0.0	7.036574487065121	0.0
50	0.0	0.0	0.0	7.43859649122807	0.0
51	0.0	0.0	0.0	7.916741005055011	0.0
52	0.0	0.0	0.0	8.47695509961344	0.0
53	0.0	0.0	0.0	9.075230449003866	0.0
54	0.0	0.0	0.0	9.6068986024383	0.0
55	0.0	0.0	0.0	10.006541778174249	0.0
56	0.0	0.0	0.0	10.488254534641689	0.0
57	0.0	0.0	0.0	10.916443651501636	0.0
58	0.0	0.0	0.0	11.294677371394588	0.0
59	0.0	0.0	0.0	11.574189711567053	0.0
60	0.0	0.0	0.0	11.935771632471008	0.0
61	0.0	0.0	0.0	12.299732381801963	0.0
62	0.0	0.0	0.0	12.655367231638419	0.0
63	0.0	0.0	0.0	13.001486767766874	0.0
64	0.0	0.0	0.0	13.418971156705323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATTGT	15	0.0022182404	69.995834	31
ACGAGCG	15	0.0022182404	69.995834	24
AGTACTA	15	0.0022182404	69.995834	40
TGGTCTT	15	0.0022182404	69.995834	12
TGGTCTA	20	7.888332E-5	69.995834	34
GACGAAT	75	0.0	60.66306	23
TTCCGAC	35	2.9438706E-7	59.996426	19
AGATCTC	135	0.0	57.03364	40
CACTTAG	25	2.3789634E-4	55.99667	52
CGAATAG	70	0.0	54.996727	25
CGTATCA	65	3.6379788E-12	53.842953	56
CTTGTCA	20	0.006931383	52.496876	1
GGCACCG	20	0.006931383	52.496876	12
AACTTGT	20	0.006931383	52.496876	24
TATTGGT	20	0.006931383	52.496876	31
GTTCGCC	20	0.006931383	52.496876	50
CGAGCGA	20	0.006931383	52.496876	25
ATTGGTC	20	0.006931383	52.496876	32
GGTCTAT	20	0.006931383	52.496876	35
TTCGCCG	20	0.006931383	52.496876	51
>>END_MODULE