FastQCFastQC Report
Wed 25 May 2016
SRR1779862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84075
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2707332.20101100208147No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG35634.23788284269997No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC21232.5251263752601845No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT6660.7921498661909009RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5490.6529884032114184No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT3030.360392506690455No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3000.35682426404995543No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT2450.2914064823074636No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT1950.23193577163247103No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1840.21885221528397267RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1590.18911685994647637No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1530.18198037466547726No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1310.15581326196848053No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1100.13083556348498365No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT1080.12845673505798394No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1050.12488849241748438No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG1040.12369907820398453No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1040.12369907820398453RNA PCR Primer, Index 48 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC900.10704727921498662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCA150.002209346370.066752
CTTGACA150.00221457470.02557
TAATACA150.00221981169.9833453
GTCGATC252.8221184E-669.98334533
TATGCAG150.00221981169.98334543
GACCACT150.00221981169.98334544
ATATCAA150.00221981169.9833456
TCGTCCA252.8221184E-669.98334531
CGTCGAT252.8221184E-669.98334532
AAGATGC150.00221981169.98334530
TCGATCT352.9475086E-759.9857234
TTCGTCG308.324725E-658.3194530
TTCGTCC308.324725E-658.3194530
TCGTCGA308.324725E-658.3194531
GTCCATC308.324725E-658.3194533
CGTCCAT308.324725E-658.3194532
TTGCATC252.3670818E-456.05336854
CATATTT252.3740638E-456.0258
CGAGACG252.3810621E-455.98667523
AATCTCG37250.054.0139936
Produced by FastQC (version 0.11.5)