##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779862_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84075
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.56767172167707	34.0	34.0	34.0	34.0	34.0
2	33.65423728813559	34.0	34.0	34.0	34.0	34.0
3	33.77191793041927	34.0	34.0	34.0	34.0	34.0
4	33.57482010110021	34.0	34.0	34.0	34.0	34.0
5	33.63094855783527	34.0	34.0	34.0	34.0	34.0
6	37.36205768658935	38.0	38.0	38.0	37.0	38.0
7	37.38684507879869	38.0	38.0	38.0	37.0	38.0
8	37.32234314600059	38.0	38.0	38.0	37.0	38.0
9	37.22246803449301	38.0	38.0	38.0	36.0	38.0
10-11	37.18124888492417	38.0	38.0	38.0	36.5	38.0
12-13	37.3390484686292	38.0	38.0	38.0	36.5	38.0
14-15	37.356901575973836	38.0	38.0	38.0	37.0	38.0
16-17	37.1623966696402	38.0	38.0	38.0	36.0	38.0
18-19	36.99386856972941	38.0	38.0	38.0	35.5	38.0
20-21	37.090282485875704	38.0	38.0	38.0	35.5	38.0
22-23	37.124406779661015	38.0	38.0	38.0	36.0	38.0
24-25	37.256669640202205	38.0	38.0	38.0	36.0	38.0
26-27	37.119209039548025	38.0	38.0	38.0	36.0	38.0
28-29	36.64542372881356	38.0	37.0	38.0	34.0	38.0
30-31	37.00290217068094	38.0	37.0	38.0	35.5	38.0
32-33	36.88718406184954	38.0	37.5	38.0	35.5	38.0
34-35	36.79191793041927	38.0	37.0	38.0	35.0	38.0
36-37	36.655052036871844	38.0	37.0	38.0	34.0	38.0
38-39	35.23510556051144	38.0	36.0	38.0	29.0	38.0
40-41	36.32846268212905	38.0	37.0	38.0	34.0	38.0
42-43	37.105869759143616	38.0	37.5	38.0	36.0	38.0
44-45	36.87190603627714	38.0	37.5	38.0	35.0	38.0
46-47	37.04017841213202	38.0	38.0	38.0	36.0	38.0
48-49	36.61769848349688	38.0	37.0	38.0	34.0	38.0
50-51	36.698792744573296	38.0	37.0	38.0	34.0	38.0
52-53	36.63004460303301	38.0	37.0	38.0	34.0	38.0
54-55	36.656669640202196	38.0	37.0	38.0	34.0	38.0
56-57	36.47992863514719	38.0	37.0	38.0	34.0	38.0
58-59	36.52483496877788	38.0	37.0	38.0	34.0	38.0
60-61	36.080053523639606	38.0	37.0	38.0	33.5	38.0
62-63	36.01123401724651	38.0	37.0	38.0	33.0	38.0
64-65	36.52994944989592	38.0	37.0	38.0	34.0	38.0
66-67	36.14221231043711	38.0	37.0	38.0	32.5	38.0
68-69	36.03341659232828	38.0	37.0	38.0	32.0	38.0
70-71	33.40982456140351	37.5	31.0	38.0	21.0	38.0
72-73	28.777639012786203	35.0	21.5	38.0	11.0	38.0
74-75	28.0709426107642	34.0	19.0	38.0	11.0	38.0
76	28.048409158489445	34.0	19.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	5.0
24	16.0
25	38.0
26	92.0
27	208.0
28	385.0
29	689.0
30	1098.0
31	1644.0
32	2626.0
33	4267.0
34	7563.0
35	17376.0
36	17978.0
37	30086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.66829314735915	12.005807726179992	12.046271392188132	54.27962773427273
2	10.56318763009218	11.60035682426405	59.12578055307761	18.71067499256616
3	11.35890573892358	13.001486767766876	57.91138864109426	17.72821885221528
4	8.09277430865299	44.73624739815641	29.021706809396374	18.149271483794234
5	8.0035682426405	25.478441867380315	57.449895926256325	9.068093963722866
6	16.519774011299436	58.62384775498067	15.129348795718109	9.727029438001784
7	14.702349093071662	22.994944989592625	15.75617008623253	46.54653583110318
8	13.401129943502823	58.15997621171572	12.016651798988997	16.422242045792448
9	13.799583705025276	14.830805828129645	11.477847160273564	59.89176330657151
10-11	14.672019030627418	35.69313113291704	32.15997621171573	17.474873624739814
12-13	14.274159976211715	13.932203389830509	36.344930121914956	35.44870651204282
14-15	13.6092774308653	12.913470115967888	16.96104668450788	56.516205768658935
16-17	34.0011894142135	32.28962236098722	12.8111804936069	20.89800773119239
18-19	35.599762117157304	19.04965804341362	13.271483794231342	32.079096045197744
20-21	15.013975617008624	14.212310437109723	13.336306868867082	57.43740707701457
22-23	13.75914362176628	34.79631281593815	13.42789176330657	38.016651798988995
24-25	34.07195955991674	34.40440083258995	13.27505203687184	18.24858757062147
26-27	35.37674695212608	36.43592030924769	14.570324115373179	13.61700862325305
28-29	14.774903360095154	18.314600059470713	32.802854594112404	34.10764198632174
30-31	13.495688373476064	13.718703538507285	57.35176925364258	15.43383883437407
32-33	30.87065120428189	14.151055605114482	20.938447814451383	34.03984537615224
34-35	17.284567350579838	14.501932798096936	34.994350282485875	33.219149568837345
36-37	12.820101100208149	51.75854891465953	16.914064823074636	18.507285162057688
38-39	13.220933690157596	20.453761522450193	34.24739815640797	32.07790663098424
40-41	13.066904549509367	13.914362176628012	37.26672613737734	35.75200713648528
42-43	33.425117009319116	13.20079215457719	35.191407620622186	18.182683215481507
44-45	14.657151352958667	32.97056199821588	37.85429675884627	14.517989889979185
46-47	32.889087124591136	14.663098424026167	20.525126375260182	31.92268807612251
48-49	31.76449598572703	12.71008028545941	16.3883437407077	39.13707998810586
50-51	16.083674841042782	13.006834119445418	36.62826315820779	34.28122788130402
52-53	11.657803000559184	13.36866902237927	56.17244292155953	18.801085055502018
54-55	11.51049600152323	13.447258187357198	40.3173790936786	34.724866717440975
56-57	31.459036194760298	13.219447469103848	20.920131141298203	34.40138519483765
58-59	13.239257307053917	14.395773717807616	53.51446537887215	18.850503596266307
60-61	31.326591314693637	32.59131469363474	21.15466983938132	14.927424152290303
62-63	12.855596140346703	57.96916084664898	14.182203238587016	14.993039774417305
64-65	11.15737381187471	60.41089209026779	13.299865573809496	15.131868524048011
66-67	11.072903156717395	60.2709327368133	13.171828706733457	15.48433539973585
68-69	11.16886824434836	59.92970018496821	13.26180436191915	15.639627208764281
70-71	11.460520446096654	56.41695167286246	13.804014869888476	18.318513011152415
72-73	13.14651454676319	47.04395257951497	15.70312825302029	24.106404620701554
74-75	14.106430181586758	42.94559534643042	17.170149111694	25.777825360288826
76	13.716603620964479	43.84412247519806	16.658339875811862	25.780934028025598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	5.5
16	5.0
17	7.0
18	16.0
19	53.5
20	82.5
21	83.0
22	94.5
23	115.0
24	138.0
25	152.0
26	189.0
27	310.5
28	410.0
29	425.0
30	477.5
31	597.5
32	778.5
33	892.0
34	924.0
35	1085.0
36	1315.0
37	1416.0
38	1608.5
39	6002.0
40	12156.0
41	12760.5
42	11412.0
43	9454.0
44	5859.0
45	3565.0
46	2908.0
47	2603.0
48	2228.0
49	2049.5
50	1941.0
51	1863.5
52	1745.0
53	1655.5
54	1607.0
55	1601.0
56	1496.5
57	1400.0
58	1402.0
59	1367.0
60	1199.0
61	976.0
62	886.0
63	825.5
64	742.0
65	660.5
66	551.5
67	501.0
68	483.5
69	427.0
70	359.0
71	330.0
72	324.5
73	303.5
74	267.5
75	247.0
76	222.0
77	178.5
78	153.0
79	146.0
80	107.5
81	72.5
82	69.5
83	63.0
84	54.0
85	33.5
86	19.5
87	17.0
88	17.0
89	10.5
90	2.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058281296461492715
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.947071067499257E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.013678263455248292
52-53	0.028545941123996433
54-55	0.051144811180493605
56-57	0.05173951828724353
58-59	0.03508771929824561
60-61	0.02973535533749628
62-63	0.030924769550996132
64-65	0.016651798988997917
66-67	0.038061254831995246
68-69	0.007731192387749033
70-71	0.01486767766874814
72-73	0.02200416294974725
74-75	0.012488849241748439
76	0.010704727921498661
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	84075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.454653583110318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.194256569464464	9.05263157894737
2	17.823483756514026	7.6479333928040445
3	13.926155893114537	8.96342551293488
4	10.15633662268544	8.71602735652691
5	6.879920168533096	7.3803151947665775
6	3.9361348264774367	5.066904549509366
7	2.2618915622574565	3.3969669937555755
8	1.1586650404701186	1.9887005649717515
9	0.5765605943009203	1.113291703835861
>10	0.9479986694755517	3.134106452572108
>50	0.03880696307794655	0.5126375260184359
>100	0.07207007428761504	2.617900683913173
>500	0.011087703736556159	1.4451382694023194
>1k	0.011087703736556159	6.763009217960154
>5k	0.0	0.0
>10k+	0.0055438518682780795	32.20101100208147
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	27073	32.20101100208147	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	3563	4.23788284269997	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	2123	2.5251263752601845	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	666	0.7921498661909009	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	549	0.6529884032114184	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	303	0.360392506690455	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	300	0.35682426404995543	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	245	0.2914064823074636	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	195	0.23193577163247103	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	184	0.21885221528397267	RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC	159	0.18911685994647637	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	153	0.18198037466547726	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	131	0.15581326196848053	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	110	0.13083556348498365	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT	108	0.12845673505798394	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	105	0.12488849241748438	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG	104	0.12369907820398453	No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT	104	0.12369907820398453	RNA PCR Primer, Index 48 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	90	0.10704727921498662	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005947071067499257	0.0
2	0.0	0.0	0.0	0.005947071067499257	0.0
3	0.0	0.0	0.0	0.005947071067499257	0.0
4	0.0	0.0	0.0	0.005947071067499257	0.0
5	0.0	0.0	0.0	0.007136485280999108	0.0
6	0.0	0.0	0.0	0.007136485280999108	0.0
7	0.0	0.0	0.0	0.007136485280999108	0.0
8	0.0	0.0	0.0	0.007136485280999108	0.0
9	0.0	0.0	0.0	0.008325899494498959	0.0
10	0.0	0.0	0.0	0.011894142134998514	0.0
11	0.0	0.0	0.0	0.013083556348498365	0.0
12	0.0	0.0	0.0	0.013083556348498365	0.0
13	0.0	0.0	0.0	0.020220041629497473	0.0
14	0.0	0.0	0.0	0.020220041629497473	0.0
15	0.0	0.0	0.0	0.020220041629497473	0.0
16	0.0	0.0	0.0	0.022598870056497175	0.0
17	0.0	0.0	0.0	0.022598870056497175	0.0
18	0.0	0.0	0.0	0.029735355337496282	0.0
19	0.0	0.0	0.0	0.039250669045495096	0.0
20	0.0	0.0	0.0	0.041629497472494795	0.0
21	0.0	0.0	0.0	0.041629497472494795	0.0
22	0.0	0.0	0.0	0.042818911685994644	0.0
23	0.0	0.0	0.0	0.0440083258994945	0.0
24	0.0	0.0	0.0	0.047576568539994056	0.0
25	0.0	0.0	0.0	0.05471305382099316	0.0
26	0.0	0.0	0.0	0.05471305382099316	0.0
27	0.0	0.0	0.0	0.07612250966399048	0.0
28	0.0	0.0	0.0	0.11775200713648529	0.0
29	0.0	0.0	0.0	0.15105560511448113	0.0
30	0.0	0.0	0.0	0.1855486173059768	0.0
31	0.0	0.0	0.0	0.2224204579244722	0.0
32	0.0	0.0	0.0	0.2604817127564674	0.0
33	0.0	0.0	0.0	0.3044900386559619	0.0
34	0.0	0.0	0.0	0.39012786202795124	0.0
35	0.0	0.0	0.0	0.48052334225393994	0.0
36	0.0	0.0	0.0	0.6779661016949152	0.0
37	0.0	0.0	0.0	1.032411537317871	0.0
38	0.0	0.0	0.0	1.4023193577163247	0.0
39	0.0	0.0	0.0	1.7888789771037763	0.0
40	0.0	0.0	0.0	2.257508177222718	0.0
41	0.0	0.0	0.0	2.7867975022301517	0.0
42	0.0	0.0	0.0	3.399345822182575	0.0
43	0.0	0.0	0.0	4.0011894142135	0.0
44	0.0	0.0	0.0	4.712459113886411	0.0
45	0.0	0.0	0.0	5.272673208444841	0.0
46	0.0	0.0	0.0	5.906630984240262	0.0
47	0.0	0.0	0.0	6.375260184359203	0.0
48	0.0	0.0	0.0	6.9104965804341365	0.0
49	0.0	0.0	0.0	7.402914064823075	0.0
50	0.0	0.0	0.0	7.816830211121023	0.0
51	0.0	0.0	0.0	8.30805828129646	0.0
52	0.0	0.0	0.0	8.867082961641392	0.0
53	0.0	0.0	0.0	9.468926553672317	0.0
54	0.0	0.0	0.0	9.980374665477253	0.0
55	0.0	0.0	0.0	10.370502527505204	0.0
56	0.0	0.0	0.0	10.858162355040143	0.0
57	0.0	0.0	0.0	11.293487957181089	0.0
58	0.0	0.0	0.0	11.67528991971454	0.0
59	0.0	0.0	0.0	11.964317573595004	0.0
60	0.0	0.0	0.0	12.31995242343146	0.0
61	0.0	0.0	0.0	12.693428486470413	0.0
62	0.0	0.0	0.0	13.060957478441868	0.0
63	0.0	0.0	0.0	13.423728813559322	0.0
64	0.0	0.0	0.0	13.865001486767767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCA	15	0.0022093463	70.0667	52
CTTGACA	15	0.002214574	70.025	57
TAATACA	15	0.002219811	69.983345	3
GTCGATC	25	2.8221184E-6	69.983345	33
TATGCAG	15	0.002219811	69.983345	43
GACCACT	15	0.002219811	69.983345	44
ATATCAA	15	0.002219811	69.983345	6
TCGTCCA	25	2.8221184E-6	69.983345	31
CGTCGAT	25	2.8221184E-6	69.983345	32
AAGATGC	15	0.002219811	69.983345	30
TCGATCT	35	2.9475086E-7	59.98572	34
TTCGTCG	30	8.324725E-6	58.31945	30
TTCGTCC	30	8.324725E-6	58.31945	30
TCGTCGA	30	8.324725E-6	58.31945	31
GTCCATC	30	8.324725E-6	58.31945	33
CGTCCAT	30	8.324725E-6	58.31945	32
TTGCATC	25	2.3670818E-4	56.053368	54
CATATTT	25	2.3740638E-4	56.02	58
CGAGACG	25	2.3810621E-4	55.986675	23
AATCTCG	3725	0.0	54.01399	36
>>END_MODULE