FastQCFastQC Report
Wed 25 May 2016
SRR1779861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206969
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT800.056.87146452
GTGTACA252.384324E-456.0100636
GCCGTAT509.749783E-1056.0100654
GTGTCGA200.00693909352.50942236
TCCCGGT200.00695237352.4840543
CGGTGGT750.051.3177446
GTCGACG305.862977E-446.6750520
ACGCTTC305.8699684E-446.66376515
ACGAATA451.6797676E-646.66376524
GGCTCGT305.876967E-446.65249328
TTACGGA404.576834E-543.75785437
CGACGAC1350.041.48892622
GTAGATC1350.041.46888438
CGCCGTA1100.041.37106353
GACGAAT602.5873123E-740.83079523
CGTATCA701.9121217E-840.00718356
TGGTGCG350.001252277440.0071835
TCGACGA350.001252277440.00718321
CTGTGTA350.001252277440.00718334
AATTGTA350.001253767239.99751323
Produced by FastQC (version 0.11.5)