##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779861_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	206969
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.485589629364785	34.0	23.0	34.0	12.0	34.0
2	29.10491909416386	34.0	24.0	34.0	12.0	34.0
3	28.808222487425653	34.0	23.0	34.0	11.0	34.0
4	29.183037073184874	34.0	24.0	34.0	12.0	34.0
5	29.49730152824819	34.0	31.0	34.0	11.0	34.0
6	32.00017877073378	37.0	28.0	38.0	12.0	38.0
7	32.16185998869396	37.0	31.0	38.0	12.0	38.0
8	31.492817764979296	37.0	27.0	38.0	11.0	38.0
9	32.48595683411526	37.0	31.0	38.0	12.0	38.0
10-11	31.09655069116631	36.5	26.0	38.0	11.0	38.0
12-13	31.907176920215104	37.0	29.5	38.0	11.0	38.0
14-15	30.755190390831476	36.5	25.5	38.0	11.0	38.0
16-17	31.336142127564997	37.0	28.0	38.0	11.0	38.0
18-19	32.16042499118225	37.0	31.0	38.0	11.0	38.0
20-21	30.96200880325073	36.5	26.5	38.0	11.0	38.0
22-23	31.800530514231596	37.0	29.0	38.0	11.0	38.0
24-25	32.12672187622301	37.0	31.0	38.0	11.0	38.0
26-27	31.040387690910233	37.0	26.0	38.0	11.0	38.0
28-29	32.212092149065796	37.0	31.0	38.0	11.0	38.0
30-31	32.62132010107794	38.0	32.5	38.0	11.0	38.0
32-33	32.72976387768216	38.0	33.5	38.0	11.0	38.0
34-35	32.64265421391609	38.0	33.0	38.0	11.0	38.0
36-37	32.16643555315047	37.5	29.0	38.0	11.0	38.0
38-39	32.09404548507264	37.0	29.0	38.0	11.0	38.0
40-41	32.06267846875619	37.0	29.0	38.0	11.0	38.0
42-43	32.476093038087825	37.5	32.5	38.0	11.0	38.0
44-45	32.51743980982659	38.0	32.0	38.0	11.0	38.0
46-47	32.43403118341394	37.5	31.5	38.0	11.0	38.0
48-49	32.107905048582154	37.0	29.0	38.0	11.0	38.0
50-51	31.50096874411144	37.0	28.5	38.0	11.0	38.0
52-53	32.06992593093651	37.0	30.5	38.0	11.0	38.0
54-55	32.20462484719934	37.0	31.5	38.0	11.0	38.0
56-57	32.2703593291749	37.0	31.0	38.0	11.0	38.0
58-59	32.51778527219052	38.0	32.0	38.0	11.0	38.0
60-61	32.4039638786485	37.0	31.0	38.0	11.0	38.0
62-63	32.410508336997324	38.0	31.5	38.0	11.0	38.0
64-65	32.40807318970474	37.5	31.5	38.0	11.0	38.0
66-67	32.30727790152149	37.0	31.0	38.0	11.0	38.0
68-69	31.680623668278827	37.0	27.5	38.0	11.0	38.0
70-71	31.721803748387437	37.0	28.5	38.0	11.0	38.0
72-73	31.939879885393466	37.0	31.0	38.0	11.0	38.0
74-75	32.03724953978615	37.0	30.0	38.0	11.0	38.0
76	30.94935473428388	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	54.0
13	622.0
14	2169.0
15	3645.0
16	4923.0
17	5856.0
18	5953.0
19	5435.0
20	4143.0
21	3347.0
22	2532.0
23	2217.0
24	1977.0
25	2068.0
26	2253.0
27	2613.0
28	3110.0
29	3790.0
30	4522.0
31	5606.0
32	7172.0
33	9323.0
34	12741.0
35	18958.0
36	32185.0
37	59752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.838033715194065	23.35857060719238	9.535727572728284	29.267668104885274
2	19.03956631186313	21.684406843536955	30.48330909459871	28.792717750001206
3	19.648353135010556	21.572795925959927	30.583324072687212	28.195526866342302
4	13.020307389029275	22.663780566171745	32.731471862936	31.584440181862984
5	15.552570674835362	33.33397755219381	33.76737579057733	17.3460759823935
6	26.48083455336996	37.638975459144476	16.282777914679578	19.597412072805987
7	26.14773545428628	28.705345709854026	21.173611144667095	23.973307691192602
8	22.506655843371455	35.99567068191591	17.591236996342303	23.90643647837032
9	23.298202886785248	17.802969899924133	18.865581343655016	40.0332458696356
10-11	22.437102283983126	26.898286167092667	24.594492735416477	26.07011881350773
12-13	22.40195971850789	23.57653459342847	25.822770131686706	28.198735556376935
14-15	24.192870395423448	25.583906690889414	22.226141239225388	27.997081674461754
16-17	25.013166350191092	26.474752013606036	23.010431614701858	25.50165002150101
18-19	21.78607235610316	26.075013589418372	25.34251374041191	26.79640031406656
20-21	23.79365992008465	25.591996869096338	24.942382675666405	25.671960535152603
22-23	24.998429671003997	25.268284669240398	24.060580876775077	25.67270478298053
24-25	21.480806816726783	28.4516685470486	23.730742191180486	26.336782445044125
26-27	22.908130725522785	28.48942831742105	24.636658807158597	23.965782149897567
28-29	21.762954945203624	27.89855422618242	26.165751783056614	24.172739045557336
30-31	22.27416249332628	26.9840579605109	26.038986609073362	24.70279293708946
32-33	20.821958844078097	28.15928955544067	25.67292686344332	25.345824737037915
34-35	21.20844607653653	28.80121762659451	25.78710862002319	24.203227676845767
36-37	23.5298950339255	26.38070016044538	25.167211149987434	24.922193655641685
38-39	23.163662985254216	25.67066378983331	26.979010875106614	24.18666234980586
40-41	21.75414014277604	27.06487733581359	26.194933987220214	24.98604853419015
42-43	19.837124579645163	26.702678674964247	27.027366549418268	26.432830195972322
44-45	19.45698733791884	25.101410199727965	28.766431593112564	26.675170869240638
46-47	20.695778220692155	25.71359545811439	27.697288156066918	25.893338165126533
48-49	20.6070005822142	26.139484994093305	27.81365280223802	25.439861621454472
50-51	22.735519719755995	26.166092891224256	26.06051820982062	25.03786917919913
52-53	18.370305579784908	27.32289685866784	27.162942863563106	27.143854697984143
54-55	18.67013295771109	26.20738310328082	27.312263373690175	27.810220565317923
56-57	19.664874660223497	27.012503775294473	26.389368770764122	26.93325279371791
58-59	19.094010650738884	27.147303004822792	26.617423912938428	27.141262431499896
60-61	18.334364367460562	26.264788895762447	27.009791602227036	28.39105513454995
62-63	19.305909242463045	26.386466100015948	26.17457609919448	28.13304855832653
64-65	20.01338688085676	25.906030862318108	25.447880571624648	28.63270168520049
66-67	18.99525173076226	28.128843621250017	24.329986588857878	28.54591805912985
68-69	19.5456807541226	29.151950292072726	23.81926761978847	27.483101334016208
70-71	19.258486194759104	29.074231102800667	24.315535939043865	27.351746763396363
72-73	19.078405365378714	27.736263417674728	24.33651516139865	28.848816055547903
74-75	18.783901652890563	27.361246348588118	23.792346109147317	30.062505889374
76	18.170660997294164	26.675202937765754	24.70332431387708	30.450811751063007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	3.0
14	4.0
15	6.0
16	18.0
17	28.0
18	40.0
19	79.0
20	100.0
21	94.0
22	102.0
23	206.0
24	388.0
25	474.0
26	556.0
27	710.0
28	977.0
29	1172.0
30	1341.5
31	1738.5
32	2399.5
33	2833.0
34	3285.5
35	4179.0
36	5249.0
37	5878.0
38	6436.5
39	7789.0
40	9202.5
41	10302.5
42	10783.0
43	11349.5
44	12323.0
45	12964.0
46	13198.0
47	13397.0
48	13225.5
49	12369.0
50	11883.0
51	11345.5
52	10158.5
53	8759.5
54	8010.0
55	7533.0
56	6674.5
57	5623.0
58	4953.0
59	4426.0
60	3579.5
61	2986.0
62	2712.0
63	2462.5
64	2034.0
65	1714.5
66	1458.0
67	1342.0
68	1210.0
69	997.0
70	893.0
71	870.0
72	763.0
73	614.5
74	568.0
75	563.0
76	505.5
77	392.0
78	312.5
79	289.0
80	264.5
81	231.5
82	198.0
83	173.0
84	137.0
85	91.0
86	74.0
87	67.0
88	54.0
89	39.0
90	32.5
91	26.5
92	25.0
93	17.5
94	11.0
95	11.5
96	11.0
97	7.5
98	6.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003865313162840812
7	0.008696954616391826
8	0.003865313162840812
9	0.01256226777923264
10-11	0.002898984872130609
12-13	2.4158207267755075E-4
14-15	4.831641453551015E-4
16-17	9.66328290710203E-4
18-19	0.00314056694480816
20-21	0.0
22-23	0.002898984872130609
24-25	0.003623731090163261
26-27	4.831641453551015E-4
28-29	0.01014644705245713
30-31	2.4158207267755075E-4
32-33	0.0
34-35	0.004348477308195913
36-37	0.021742386540979566
38-39	0.01377017814262039
40-41	7.247462180326522E-4
42-43	4.831641453551015E-4
44-45	0.006039551816938769
46-47	0.00314056694480816
48-49	2.4158207267755075E-4
50-51	0.00314056694480816
52-53	0.016669163014751003
54-55	0.011354357415844885
56-57	0.015219670578685698
58-59	0.016910745087428552
60-61	0.02754035628524078
62-63	0.010629611197812232
64-65	0.024158207267755076
66-67	0.02488295348578773
68-69	0.0
70-71	2.4158207267755075E-4
72-73	0.006039551816938769
74-75	0.01377017814262039
76	0.004348477308195913
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	206969.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.97146230641167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.04970755547656	43.32663047410465
2	8.782599418589477	10.007150634569669
3	5.291518103277585	9.043965724937216
4	3.4532175009653976	7.869394027683652
5	2.136101583372131	6.084841541987583
6	1.322838627323523	4.521843059641648
7	0.7609080499373575	3.034503099995596
8	0.4830889780079794	2.201782840097963
9	0.35412428156769654	1.8157480343207226
>10	1.3619281596574957	11.963597811585007
>50	0.003967741824775738	0.13054275107630525
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0033821490174857105	0.0
6	0.0	0.0	0.0	0.0033821490174857105	0.0
7	0.0	0.0	0.0	0.0033821490174857105	0.0
8	0.0	0.0	0.0	0.0033821490174857105	0.0
9	0.0	0.0	0.0	0.005797969744261218	0.0
10	0.0	0.0	0.0	0.005797969744261218	0.0
11	0.0	0.0	0.0	0.005797969744261218	0.0
12	0.0	0.0	0.0	0.005797969744261218	0.0
13	0.0	0.0	0.0	0.006764298034971421	0.0
14	0.0	0.0	0.0	0.00966328290710203	0.0
15	0.0	0.0	0.0	0.011112775343167335	0.0
16	0.0	0.0	0.0	0.011112775343167335	0.0
17	0.0	0.0	0.0	0.011595939488522436	0.0
18	0.0	0.0	0.0	0.011595939488522436	0.0
19	0.0	0.0	0.0	0.01304543192458774	0.0
20	0.0	0.0	0.0	0.013528596069942842	0.0
21	0.0	0.0	0.0	0.014978088506008146	0.0
22	0.0	0.0	0.0	0.03430465432021221	0.0
23	0.0	0.0	0.0	0.03623731090163261	0.0
24	0.0	0.0	0.0	0.04445110137266934	0.0
25	0.0	0.0	0.0	0.04638375795408974	0.0
26	0.0	0.0	0.0	0.04638375795408974	0.0
27	0.0	0.0	0.0	0.06522715962293871	0.0
28	0.0	0.0	0.0	0.09083485932675908	0.0
29	0.0	0.0	0.0	0.12320685706555087	0.0
30	0.0	0.0	0.0	0.16814112258357533	0.0
31	0.0	0.0	0.0	0.20824374664804873	0.0
32	0.0	0.0	0.0	0.25269484802071807	0.0
33	0.0	0.0	0.0	0.2937638003759017	0.0
34	0.0	0.0	0.0	0.36237310901632613	0.0
35	0.0	0.0	0.0	0.4536911324884403	0.0
36	0.0	0.0	0.0	0.6435746416129952	0.0
37	0.0	0.0	0.0	1.0354207634959824	0.0
38	0.0	0.0	0.0	1.4494924360653045	0.0
39	0.0	0.0	0.0	1.9737255337755897	0.0
40	0.0	0.0	0.0	2.576231223033401	0.0
41	0.0	0.0	0.0	3.313539708845286	0.0
42	0.0	0.0	0.0	4.085152848977383	0.0
43	0.0	0.0	0.0	4.796853635085448	0.0
44	0.0	0.0	0.0	5.601805101247047	0.0
45	0.0	0.0	0.0	6.385497345013022	0.0
46	0.0	0.0	0.0	7.262923432977885	0.0
47	0.0	0.0	0.0	8.079954002773363	0.0
48	0.0	0.0	0.0	8.910996332784137	0.0
49	0.0	0.0	0.0	9.675845174881262	0.0
50	0.0	0.0	0.0	10.42088428701883	0.0
51	0.0	0.0	0.0	11.23066739463398	0.0
52	0.0	0.0	0.0	12.037551517376999	0.0
53	0.0	0.0	0.0	12.83042387990472	0.0
54	0.0	0.0	0.0	13.585609439094744	0.0
55	0.0	0.0	0.0	14.327266402214825	0.0
56	0.0	0.0	0.0	14.95006498557755	0.0
57	0.0	0.0	0.0	15.587841657446285	0.0
58	0.0	0.0	0.0	16.190347346704097	0.0
59	0.0	0.0	0.0	16.790920379380488	0.0
60	0.0	0.0	0.0	17.38617860645797	0.0
61	0.0	0.0	0.0	17.944233194343113	0.0
62	0.0	0.0	0.0	18.49600664833864	0.0
63	0.0	0.0	0.0	19.080635264218312	0.0
64	0.0	0.0	0.0	19.684107281766835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	80	0.0	56.871464	52
GTGTACA	25	2.384324E-4	56.01006	36
GCCGTAT	50	9.749783E-10	56.01006	54
GTGTCGA	20	0.006939093	52.509422	36
TCCCGGT	20	0.006952373	52.48405	43
CGGTGGT	75	0.0	51.31774	46
GTCGACG	30	5.862977E-4	46.67505	20
ACGCTTC	30	5.8699684E-4	46.663765	15
ACGAATA	45	1.6797676E-6	46.663765	24
GGCTCGT	30	5.876967E-4	46.652493	28
TTACGGA	40	4.576834E-5	43.757854	37
CGACGAC	135	0.0	41.488926	22
GTAGATC	135	0.0	41.468884	38
CGCCGTA	110	0.0	41.371063	53
GACGAAT	60	2.5873123E-7	40.830795	23
CGTATCA	70	1.9121217E-8	40.007183	56
TGGTGCG	35	0.0012522774	40.007183	5
TCGACGA	35	0.0012522774	40.007183	21
CTGTGTA	35	0.0012522774	40.007183	34
AATTGTA	35	0.0012537672	39.997513	23
>>END_MODULE