FastQCFastQC Report
Wed 25 May 2016
SRR1779861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206969
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2637512.743454333740804No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG35261.703636776522088No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC20010.9668114548555582No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT7000.33821490174857105TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5620.2715382496895671No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT4280.20679425421198344No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT4150.20051312032236712No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2380.11499306659451414No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA150.002225242869.97681441
TCTGTAC150.002225242869.9768143
CTGCCGT150.002225242869.97681444
CGAGAAC150.002225242869.97681428
CGCACGT301.0224903E-769.97681430
AAACGAC252.3846493E-456.0085170
CGGCTTA252.3903532E-455.98144534
TAACTCG653.6379788E-1253.82831236
ATCTCGT36900.053.6678537
CTTGAAA36900.053.54289257
ACGTATC36800.053.52845433
CGTATCT36750.053.50607734
GTATCTC36750.053.50607735
GCCGTCT38300.053.4805846
TTGAAAA36900.053.4350358
TATGCCG38450.053.4153343
CTCGTAT38300.053.3504639
CCGTCTT38450.053.27194647
TCTCGTA37050.053.261738
CTGCTTG38550.053.2503354