FastQCFastQC Report
Wed 25 May 2016
SRR1779860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173026
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTC150.002222648469.9924858
CTGTGCA150.002222648469.9924859
GATCTCG800.056.86889341
GCCGTAT452.681736E-854.45433854
CGGTTAC200.006937118752.5095458
GTACTGG200.006945056452.494366
CCGCGAT200.006945056452.4943619
TCGGTGG750.051.32782445
CGTATCA552.2646418E-950.91834656
GATGTAC352.0813808E-549.9946331
CTCGGTG707.2759576E-1249.99463344
TCTCGGT707.2759576E-1249.99463343
CGCCGTA950.047.9034453
AGATCTC1400.047.494940
CGTACCA451.6753384E-646.67514856
ATGTATC305.8685924E-446.6616554
GACGAAT305.8685924E-446.66165523
GTTATAC305.8685924E-446.6616551
CGGTGGT851.8189894E-1245.28925346
GTAGATC1400.044.99516738
Produced by FastQC (version 0.11.5)