FastQCFastQC Report
Wed 25 May 2016
SRR1779860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173026
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT163239.433842312716008No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG22031.2732190537838244No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC12540.7247465698796713No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT4550.26296625940610086RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4360.25198525077156037No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT2930.16933871210107151No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT2770.1600915469351427No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1920.11096598199114584No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG150.0022202170.01185663
TCGCAAC150.002222757669.9916219
GTCTACC150.002222757669.9916242
AACTCGT452.6882844E-854.43792337
ATCTCGT24300.054.00587537
GATCTCG24300.053.86186236
CTCGTAT25500.053.79747839
TCTCGTA24400.053.78454238
TCGTATG25650.053.75574540
CGTATGC25650.053.6193141
TATGCCG25400.053.45816443
ATGCCGT25350.053.42555644
AGATCTC24400.053.21084235
TAGATCT24450.053.10202834
CGTCTTC25250.053.09809548
GTATGCC25700.052.97031442
GCCGTCT25400.052.9223446
CCGTCTT25350.052.8886447
TGCCGTC25500.052.85208545
GTCTTCT25650.052.8159649