FastQCFastQC Report
Wed 25 May 2016
SRR1779859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127628
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA352.958095E-759.990618
GCAGTGT252.3848085E-455.9912268
GATATCG200.006931497752.5123541
GTCATCG407.439594E-752.4917684
CCGTGCT200.00694225552.49176870
CCGTACC407.439594E-752.49176855
GAAGTAT200.00694225552.4917686
AACTGGT200.00694225552.49176816
CGTAAGG200.00694225552.4917681
TGTGTCG200.00694225552.49176835
ACGGTGC200.00694225552.49176817
GACGCTT552.2628228E-950.90111514
TCCGACG506.1510946E-848.9923220
TATAGAT305.852871E-446.6776439
ATCGGTG305.852871E-446.6776444
TTCCGAC451.6757585E-646.6593519
TCGGTGG1050.043.32653845
CGACGAG503.4602617E-641.9934222
ATCGTCA350.001252543539.9937327
AGTCTCT350.001252543539.9937327
Produced by FastQC (version 0.11.5)