##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779859_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127628
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.864669194847526	33.0	23.0	34.0	12.0	34.0
2	28.535783683831134	34.0	23.0	34.0	12.0	34.0
3	28.1722662738584	34.0	23.0	34.0	11.0	34.0
4	28.509269125897138	34.0	23.0	34.0	12.0	34.0
5	28.909533958065627	34.0	23.0	34.0	11.0	34.0
6	31.35579183251324	37.0	27.0	38.0	12.0	38.0
7	31.539121509386653	37.0	27.0	38.0	12.0	38.0
8	30.793211520982858	37.0	26.0	38.0	11.0	38.0
9	31.64765568683988	37.0	27.0	38.0	11.0	38.0
10-11	30.271202244021687	36.5	24.5	38.0	11.0	38.0
12-13	31.006017488325448	37.0	26.0	38.0	11.0	38.0
14-15	29.88998103864356	35.5	23.5	38.0	11.0	38.0
16-17	30.439570470429686	36.5	24.5	38.0	11.0	38.0
18-19	31.134374902059108	37.0	26.0	38.0	11.0	38.0
20-21	30.04993418372144	36.0	23.5	38.0	11.0	38.0
22-23	30.837539568119848	37.0	25.0	38.0	11.0	38.0
24-25	31.11797176168239	37.0	26.0	38.0	11.0	38.0
26-27	30.09796439652741	36.5	23.0	38.0	11.0	38.0
28-29	31.123887391481496	37.0	26.0	38.0	11.0	38.0
30-31	31.50277760366064	37.0	27.0	38.0	11.0	38.0
32-33	31.640980035728838	37.5	27.0	38.0	11.0	38.0
34-35	31.57037640643119	37.0	27.0	38.0	11.0	38.0
36-37	31.12007161437929	37.0	26.0	38.0	11.0	38.0
38-39	31.007231955370294	37.0	25.5	38.0	11.0	38.0
40-41	30.963996928573668	37.0	26.0	38.0	11.0	38.0
42-43	31.324004137023223	37.0	26.5	38.0	11.0	38.0
44-45	31.37430266085812	37.0	27.0	38.0	11.0	38.0
46-47	31.26580765976118	37.0	26.5	38.0	11.0	38.0
48-49	30.978899614504655	37.0	25.5	38.0	11.0	38.0
50-51	30.426105556774374	36.5	24.5	38.0	11.0	38.0
52-53	30.937740934591154	37.0	26.0	38.0	11.0	38.0
54-55	31.0713244742533	37.0	25.5	38.0	11.0	38.0
56-57	31.107715391606856	37.0	25.5	38.0	11.0	38.0
58-59	31.29647882909706	37.0	26.0	38.0	11.0	38.0
60-61	31.214353433415866	37.0	26.0	38.0	11.0	38.0
62-63	31.204003040085247	37.0	26.0	38.0	11.0	38.0
64-65	31.20752499451531	37.0	26.0	38.0	11.0	38.0
66-67	31.136756824521264	37.0	25.0	38.0	11.0	38.0
68-69	30.51949807252327	37.0	24.5	38.0	11.0	38.0
70-71	30.531419437740936	37.0	24.5	38.0	11.0	38.0
72-73	30.757721659823865	37.0	25.0	38.0	11.0	38.0
74-75	30.848849782179457	37.0	25.0	38.0	11.0	38.0
76	29.729761494342934	36.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	37.0
13	456.0
14	1528.0
15	2875.0
16	3953.0
17	4576.0
18	4698.0
19	4306.0
20	3382.0
21	2570.0
22	2059.0
23	1610.0
24	1522.0
25	1498.0
26	1551.0
27	1786.0
28	2113.0
29	2396.0
30	2852.0
31	3430.0
32	4213.0
33	5347.0
34	7220.0
35	10563.0
36	17766.0
37	33317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.38834738458646	17.603503933306172	8.904785783683831	36.10336289842354
2	20.082741112773945	21.96948921466461	30.879829503161556	27.06794016939989
3	22.139342464036105	19.527846554047702	30.57322844516877	27.759582536747423
4	14.27273012191682	22.937756605133668	29.973046666875607	32.8164666060739
5	14.917572946375405	29.79753659071677	34.82307957501489	20.461810887892938
6	25.863473954742055	39.563091581520716	16.24616059675296	18.327273866984267
7	24.912041499173306	32.04118573544277	17.3002029510175	25.746569814366428
8	21.992289730610707	35.84413345661407	16.82155114321982	25.342025669555408
9	23.771763489053612	18.896428515459718	17.484446255347823	39.84736174013885
10-11	22.34201762977473	27.214495592556315	22.93281096963761	27.510675808031344
12-13	22.737565424514997	22.740699533017832	24.520873162628888	30.000861879838283
14-15	25.624772284752773	24.793048465639973	21.095148734784704	28.487030514822546
16-17	26.71681259916554	24.889228418640183	20.87872911402323	27.515229868171044
18-19	22.746202697779015	24.91155625727237	23.70331482838192	28.638926216566695
20-21	25.294214435703765	24.11069671232018	22.896621431033942	27.698467420942112
22-23	26.92636181282517	23.830549113019494	21.591550178649786	27.651538895505546
24-25	22.79786250450535	27.493222384153697	21.443514644351463	28.265400466989487
26-27	24.490507494495677	27.484388099696773	22.43373267412068	25.59137173168687
28-29	22.700648422042434	26.522616412325895	24.06135522165847	26.7153799439732
30-31	23.46702917855016	25.784702416397653	24.022549910677906	26.725718494374274
32-33	21.917996051023287	26.862835741373363	23.8666280126618	27.35254019494155
34-35	21.65658496834451	28.50874443678305	23.253463298439165	26.581207296433274
36-37	24.344561950699617	24.870793186814048	23.736829525373125	27.047815337113214
38-39	24.410149118064208	24.135107391648447	25.112249935353443	26.342493554933906
40-41	22.734609447844107	26.09831775408025	24.298541844594013	26.868530953481628
42-43	20.416838063896886	25.585394997159703	25.29117940882647	28.706587530116938
44-45	20.361381115250957	23.730719353713912	27.400163765509728	28.5077357655254
46-47	21.07564289408114	24.910284738219485	26.3273157507091	27.686756616990266
48-49	21.257482684050522	25.492446798508166	25.638966371015766	27.611104146425546
50-51	24.093633692458376	25.560822722820763	23.698726738491676	26.64681684622919
52-53	18.781296283235772	27.168112466793616	25.513866359483117	28.5367248904875
54-55	19.855507974878446	25.299033454918725	25.118810213172754	29.72664835703008
56-57	20.668280947567215	26.10276543558157	24.21029535071428	29.01865826613693
58-59	19.80887002926898	26.13520153280124	24.893914638016764	29.162013799913016
60-61	18.99537223891943	25.251274495589715	25.617655241595777	30.13569802389508
62-63	20.557155451072585	25.519443628171224	24.408659026349298	29.5147418944069
64-65	20.7239897020733	24.841984819570765	23.705607824543392	30.728417653812546
66-67	19.706902294234048	28.230638114457008	22.109676534550655	29.952783056758292
68-69	19.63362271601843	29.348967311248312	21.787147021029867	29.230262951703388
70-71	19.576110164345458	29.314606961665785	22.44187185363656	28.667411020352198
72-73	19.039801911149944	28.42551490955536	22.62115115636717	29.91353202292753
74-75	18.939503759221747	27.389231269516024	21.98801907232045	31.683245898941774
76	18.240156709108717	26.488540646425072	23.294025465230167	31.97727717923604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	2.0
14	9.5
15	14.0
16	25.0
17	37.0
18	45.0
19	75.0
20	125.5
21	154.0
22	172.5
23	254.0
24	353.5
25	390.0
26	463.0
27	641.0
28	836.0
29	926.0
30	1024.5
31	1270.5
32	1586.5
33	1755.0
34	1997.5
35	2403.0
36	2831.5
37	3097.0
38	3312.5
39	3907.0
40	4403.5
41	4838.0
42	5155.0
43	5379.0
44	5846.5
45	6115.0
46	6140.0
47	6310.5
48	6472.0
49	6307.0
50	6151.0
51	6103.5
52	5830.0
53	5344.0
54	5084.0
55	4897.0
56	4581.5
57	4197.0
58	3941.0
59	3716.0
60	3243.5
61	2801.0
62	2606.0
63	2470.5
64	2185.0
65	1925.5
66	1765.5
67	1715.0
68	1579.0
69	1337.5
70	1189.0
71	1146.0
72	1066.5
73	976.5
74	905.5
75	845.0
76	821.0
77	707.5
78	570.0
79	522.0
80	498.5
81	425.5
82	368.0
83	360.0
84	305.0
85	226.0
86	163.5
87	125.0
88	111.0
89	86.5
90	63.0
91	40.0
92	30.0
93	24.5
94	23.5
95	22.0
96	16.0
97	14.0
98	9.5
99	8.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.83527125709092E-4
3	0.0
4	0.0
5	0.0
6	0.003134108502836368
7	0.008618798382800013
8	0.004701162754254552
9	0.004701162754254552
10-11	0.002350581377127276
12-13	0.0
14-15	0.001175290688563638
16-17	3.91763562854546E-4
18-19	0.0035258720656909143
20-21	0.0
22-23	0.003134108502836368
24-25	0.001567054251418184
26-27	7.83527125709092E-4
28-29	0.008227034819945465
30-31	0.0
32-33	0.0
34-35	0.003134108502836368
36-37	0.017629360328454573
38-39	0.008618798382800013
40-41	7.83527125709092E-4
42-43	3.91763562854546E-4
44-45	0.005092926317109099
46-47	0.001567054251418184
48-49	0.0
50-51	0.002350581377127276
52-53	0.013319961137054566
54-55	0.006659980568527283
56-57	0.013319961137054566
58-59	0.014495251825618203
60-61	0.022330523082709122
62-63	0.012144670448490926
64-65	0.023114050208418217
66-67	0.019979941705581847
68-69	0.0
70-71	3.91763562854546E-4
72-73	0.006659980568527283
74-75	0.006659980568527283
76	0.002350581377127276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	127628.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.93261666718902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60678621843466	61.89942645814398
2	8.59727087363413	12.884320055160309
3	4.731531343127516	10.636380731500925
4	2.2899566058451404	6.863697621211647
5	1.0111360903434934	3.7883536528034596
6	0.4297589794531291	1.932177891998621
7	0.18089611543890835	0.9488513492337104
8	0.08156009829037486	0.4889209264424734
9	0.03659748000209128	0.246811044598364
>10	0.03450619543054321	0.31106026890650956
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004701162754254552	0.0
2	0.0	0.0	0.0	0.004701162754254552	0.0
3	0.0	0.0	0.0	0.004701162754254552	0.0
4	0.0	0.0	0.0	0.02037170526843639	0.0
5	0.0	0.0	0.0	0.02350581377127276	0.0
6	0.0	0.0	0.0	0.02350581377127276	0.0
7	0.0	0.0	0.0	0.02350581377127276	0.0
8	0.0	0.0	0.0	0.02663992227410913	0.0
9	0.0	0.0	0.0	0.02663992227410913	0.0
10	0.0	0.0	0.0	0.027423449399818223	0.0
11	0.0	0.0	0.0	0.027423449399818223	0.0
12	0.0	0.0	0.0	0.027423449399818223	0.0
13	0.0	0.0	0.0	0.028990503651236406	0.0
14	0.0	0.0	0.0	0.028990503651236406	0.0
15	0.0	0.0	0.0	0.031341085028363684	0.0
16	0.0	0.0	0.0	0.031341085028363684	0.0
17	0.0	0.0	0.0	0.03212461215407277	0.0
18	0.0	0.0	0.0	0.033691666405490955	0.0
19	0.0	0.0	0.0	0.036042247782618234	0.0
20	0.0	0.0	0.0	0.04309399191400006	0.0
21	0.0	0.0	0.0	0.04701162754254552	0.0
22	0.0	0.0	0.0	0.0689503870624001	0.0
23	0.0	0.0	0.0	0.06973391418810919	0.0
24	0.0	0.0	0.0	0.07286802269094556	0.0
25	0.0	0.0	0.0	0.08070329394803648	0.0
26	0.0	0.0	0.0	0.08697151095370921	0.0
27	0.0	0.0	0.0	0.14651957250760023	0.0
28	0.0	0.0	0.0	0.17864418466167298	0.0
29	0.0	0.0	0.0	0.20058294418152756	0.0
30	0.0	0.0	0.0	0.22878992070705487	0.0
31	0.0	0.0	0.0	0.26796627699250947	0.0
32	0.0	0.0	0.0	0.3094932146550914	0.0
33	0.0	0.0	0.0	0.3768765474660733	0.0
34	0.0	0.0	0.0	0.4348575547685461	0.0
35	0.0	0.0	0.0	0.5578713135048735	7.83527125709092E-4
36	0.0	0.0	0.0	0.7874447613376375	7.83527125709092E-4
37	0.0	0.0	0.0	1.1196602626382925	7.83527125709092E-4
38	0.0	0.0	0.0	1.4941862287272385	7.83527125709092E-4
39	0.0	0.0	0.0	1.9549001786441846	7.83527125709092E-4
40	0.0	0.0	0.0	2.428150562572476	7.83527125709092E-4
41	0.0	0.0	0.0	3.157614316607641	7.83527125709092E-4
42	0.0	0.0	0.0	3.9513272949509513	7.83527125709092E-4
43	0.0	0.0	0.0	4.866486977779171	7.83527125709092E-4
44	0.0	0.0	0.0	5.616322437082772	7.83527125709092E-4
45	0.0	0.0	0.0	6.294856927946846	7.83527125709092E-4
46	0.0	0.0	0.0	6.862130566960229	7.83527125709092E-4
47	0.0	0.0	0.0	7.501488701538848	7.83527125709092E-4
48	0.0	0.0	0.0	8.158084432883067	7.83527125709092E-4
49	0.0	0.0	0.0	8.602344313160122	7.83527125709092E-4
50	0.0	0.0	0.0	9.155514463910741	7.83527125709092E-4
51	0.0	0.0	0.0	9.818378412260632	7.83527125709092E-4
52	0.0	0.0	0.0	10.672422979283542	7.83527125709092E-4
53	0.0	0.0	0.0	11.454383050741217	7.83527125709092E-4
54	0.0	0.0	0.0	12.064750681668599	7.83527125709092E-4
55	0.0	0.0	0.0	12.721346413012819	7.83527125709092E-4
56	0.0	0.0	0.0	13.296455323283292	7.83527125709092E-4
57	0.0	0.0	0.0	13.895070047325039	7.83527125709092E-4
58	0.0	0.0	0.0	14.451374306578494	7.83527125709092E-4
59	0.0	0.0	0.0	14.923057636255367	7.83527125709092E-4
60	0.0	0.0	0.0	15.350863446892532	7.83527125709092E-4
61	0.0	0.0	0.0	15.805309179803805	7.83527125709092E-4
62	0.0	0.0	0.0	16.251919641457988	7.83527125709092E-4
63	0.0	0.0	0.0	16.729087661014823	7.83527125709092E-4
64	0.0	0.0	0.0	17.257184943742754	7.83527125709092E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCCGA	35	2.958095E-7	59.9906	18
GCAGTGT	25	2.3848085E-4	55.991226	8
GATATCG	20	0.0069314977	52.51235	41
GTCATCG	40	7.439594E-7	52.491768	4
CCGTGCT	20	0.006942255	52.491768	70
CCGTACC	40	7.439594E-7	52.491768	55
GAAGTAT	20	0.006942255	52.491768	6
AACTGGT	20	0.006942255	52.491768	16
CGTAAGG	20	0.006942255	52.491768	1
TGTGTCG	20	0.006942255	52.491768	35
ACGGTGC	20	0.006942255	52.491768	17
GACGCTT	55	2.2628228E-9	50.901115	14
TCCGACG	50	6.1510946E-8	48.99232	20
TATAGAT	30	5.852871E-4	46.67764	39
ATCGGTG	30	5.852871E-4	46.67764	44
TTCCGAC	45	1.6757585E-6	46.65935	19
TCGGTGG	105	0.0	43.326538	45
CGACGAG	50	3.4602617E-6	41.99342	22
ATCGTCA	35	0.0012525435	39.993732	7
AGTCTCT	35	0.0012525435	39.993732	7
>>END_MODULE