FastQCFastQC Report
Wed 25 May 2016
SRR1779859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127628
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2091616.38825336133137No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG27832.1805559908484033No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC14621.1455166577866926No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT4980.3901965086031278Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4630.3627730592033096No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT3800.297740307769455No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT3640.2852038737581095No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1890.1480866267590184No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1550.12144670448490927No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1350.10577616197072744No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1330.10420910771930925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTA553.45608E-1157.26951638
ATATCGT252.3836212E-455.9968737
ACTCGTA509.713403E-1055.9968738
CTCGTAT29950.053.63639539
TATGCCG30450.053.5602243
CGTCTTC30150.053.5127648
TGCCGTC30500.053.47241245
TCGTATG30350.053.3907540
GTATGCC30550.053.384942
ATGCCGT30550.053.384944
GCCGTCT30500.053.35766646
CGTATGC30450.053.3303541
CTGCTTG29900.053.27876754
TGCTTGA29800.053.22257255
TCTCGTA28700.053.1677638
ATCTCGT28650.053.13838637
TCTGCTT29950.053.07291853
CCGTCTT30550.053.0412247
GATCTCG28650.053.01622836
GCTTGAA30050.052.896356