##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779859_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127628
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.54766979032814	34.0	34.0	34.0	34.0	34.0
2	33.62225373742439	34.0	34.0	34.0	34.0	34.0
3	33.752162534866955	34.0	34.0	34.0	34.0	34.0
4	33.5533190209045	34.0	34.0	34.0	34.0	34.0
5	33.590379853950544	34.0	34.0	34.0	34.0	34.0
6	37.272095464945	38.0	38.0	38.0	36.0	38.0
7	37.34779202055975	38.0	38.0	38.0	37.0	38.0
8	37.248182217068354	38.0	38.0	38.0	36.0	38.0
9	37.14516407058012	38.0	38.0	38.0	36.0	38.0
10-11	37.10722960478891	38.0	38.0	38.0	35.5	38.0
12-13	37.26959601341399	38.0	38.0	38.0	36.5	38.0
14-15	37.31161265553013	38.0	38.0	38.0	36.5	38.0
16-17	37.19022471557965	38.0	38.0	38.0	36.0	38.0
18-19	37.057013351302224	38.0	38.0	38.0	35.5	38.0
20-21	37.074826840505224	38.0	38.0	38.0	35.5	38.0
22-23	37.07859168834425	38.0	38.0	38.0	36.0	38.0
24-25	37.23275848559877	38.0	38.0	38.0	36.0	38.0
26-27	37.11334503400508	38.0	38.0	38.0	36.0	38.0
28-29	36.61798351458927	38.0	37.0	38.0	34.0	38.0
30-31	36.97162848277807	38.0	37.5	38.0	35.5	38.0
32-33	36.97252170370138	38.0	38.0	38.0	35.5	38.0
34-35	37.04763844924311	38.0	38.0	38.0	36.0	38.0
36-37	37.002985238348955	38.0	38.0	38.0	36.0	38.0
38-39	35.88013210267339	38.0	37.0	38.0	32.5	38.0
40-41	36.58681480552857	38.0	37.0	38.0	34.5	38.0
42-43	37.11569169774658	38.0	37.5	38.0	36.0	38.0
44-45	36.87735058137713	38.0	37.5	38.0	35.0	38.0
46-47	37.0478970131946	38.0	38.0	38.0	36.0	38.0
48-49	36.665214529727024	38.0	37.0	38.0	34.5	38.0
50-51	36.79768154323503	38.0	37.0	38.0	34.5	38.0
52-53	36.71956388880183	38.0	37.0	38.0	34.5	38.0
54-55	36.73830585764879	38.0	37.0	38.0	34.5	38.0
56-57	36.59636600119096	38.0	37.0	38.0	34.0	38.0
58-59	36.660979565612564	38.0	37.0	38.0	34.0	38.0
60-61	36.31743817970978	38.0	37.0	38.0	34.0	38.0
62-63	36.145387375810955	38.0	37.0	38.0	34.0	38.0
64-65	36.5557479549942	38.0	37.0	38.0	34.0	38.0
66-67	36.26308490299934	38.0	37.0	38.0	33.0	38.0
68-69	36.30652364684865	38.0	37.0	38.0	34.0	38.0
70-71	34.99925173159495	38.0	35.5	38.0	27.5	38.0
72-73	32.6335913749334	38.0	30.0	38.0	16.0	38.0
74-75	32.287260632463095	38.0	29.5	38.0	11.0	38.0
76	32.088499388848845	38.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	10.0
23	18.0
24	40.0
25	68.0
26	178.0
27	281.0
28	509.0
29	848.0
30	1253.0
31	2114.0
32	3285.0
33	5053.0
34	8672.0
35	18220.0
36	22320.0
37	64758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.66714220765987	12.988539984636368	10.756894037970119	40.58742376973365
2	18.499075437991664	16.86307079951108	41.97276459711035	22.665089165386906
3	17.352775253079262	16.22371266493246	43.1574576111825	23.26605447080578
4	12.556805716613908	31.11699627041088	29.985583100886952	26.340614912088256
5	12.273168897107217	27.75331431974175	45.088068448929704	14.88544833422133
6	24.4139217099696	45.26514557933996	17.179615758297555	13.141316952392893
7	23.283292067571377	25.359638950700475	16.97354812423606	34.38352085749209
8	20.646723289560285	45.25652678095716	15.236468486539003	18.860281442943556
9	20.05516030964992	17.012724480521516	15.272510734321623	47.659604475506946
10-11	20.757592377848123	29.6533675995863	28.51764503087097	21.07139499169461
12-13	20.701570188359923	18.021907418434825	30.213981258031154	31.062541135174097
14-15	19.25126147867239	17.491067790766916	19.429513899771212	43.82815683078948
16-17	29.962469050678536	28.946234368633846	17.798210424044882	23.293086156642744
18-19	30.348356160090262	21.75384711818723	19.186228727238536	28.711567994483968
20-21	19.8682890901683	19.511784247970667	18.855188516626445	41.76473814523459
22-23	19.491804306265085	29.935437364841572	18.793681637258285	31.779076691635066
24-25	30.148363767428922	29.801254451500682	18.587194565349403	21.463187215720996
26-27	30.872144043626793	30.969301407214715	18.8081768890839	19.350377660074592
28-29	29.261604036731754	21.61751339831385	19.261839094869465	29.859043470084934
30-31	21.34876359419563	19.590920487667283	38.99261917447582	20.067696743661266
32-33	19.012677468893973	39.30838060613658	22.29408907136365	19.384852853605793
34-35	18.807785125521047	31.951452659291064	19.261839094869465	29.978923120318424
36-37	29.25572758328893	30.744037358573355	19.529805371861976	20.47042968627574
38-39	19.992478139593192	21.546604193437176	29.819083586673774	28.641834080295858
40-41	18.540602375654245	18.800733381389666	31.383395493151973	31.27526874980412
42-43	29.176428185258608	18.081988920839635	30.703926285190438	22.037656608711323
44-45	19.15214529727019	28.356238442974895	32.25585294762905	20.235763312125865
46-47	28.201883599210202	18.814445106089572	23.7675118312596	29.216159463440622
48-49	27.338436706678788	17.882831353621462	21.846303319020905	32.93242862067885
50-51	19.16194178105004	18.45977218671755	32.18342613309092	30.19485989914149
52-53	16.755884035707847	18.734863744307983	42.47321519542914	22.036037024555025
54-55	16.405034315973488	18.789318344837316	33.999270957045255	30.806376382143934
56-57	26.774514722945213	18.587522343127723	23.77583179152686	30.8621311424002
58-59	17.24766327670331	18.809397840612938	40.975839162894594	22.967099719789154
60-61	26.620529118775448	28.52625020083941	23.91967991096446	20.93354076942068
62-63	17.02896420915678	41.72162851667666	20.093503724865485	21.155903549301073
64-65	16.40289799420871	43.12705957031633	19.0481601498368	21.42188228563816
66-67	16.581949577329002	42.876906134510094	18.707649620046794	21.833494668114106
68-69	16.41885776748669	42.933038705203394	18.48086288429969	22.16724064301022
70-71	16.81281394237174	40.950623378862325	18.860834881005555	23.375727797760383
72-73	17.729162503281724	36.59468419010897	19.864342224364325	25.811811082244972
74-75	18.403545065588414	35.20812763489899	20.03032582632	26.358001473192594
76	18.27485952964963	35.34837430548481	19.87900350294263	26.497762661922934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	4.5
15	12.5
16	32.5
17	46.0
18	56.5
19	87.0
20	130.5
21	154.0
22	203.5
23	269.5
24	380.5
25	475.0
26	517.5
27	695.0
28	918.0
29	1006.0
30	1051.5
31	1232.0
32	1459.0
33	1551.0
34	1697.0
35	2054.0
36	2358.0
37	2451.0
38	2827.5
39	6805.5
40	11846.5
41	12336.0
42	11386.0
43	9683.0
44	7081.0
45	5628.5
46	5075.0
47	4853.5
48	4530.5
49	4282.5
50	4136.0
51	4034.5
52	3782.0
53	3484.5
54	3338.0
55	3203.5
56	3012.0
57	2931.0
58	2907.0
59	2684.0
60	2286.5
61	2021.5
62	1931.0
63	1896.0
64	1770.0
65	1592.5
66	1454.0
67	1402.0
68	1319.5
69	1146.5
70	1077.0
71	1098.0
72	1017.0
73	917.0
74	888.5
75	879.0
76	817.5
77	679.5
78	518.5
79	434.0
80	438.5
81	404.5
82	337.0
83	308.0
84	270.5
85	195.0
86	147.5
87	138.0
88	115.5
89	75.0
90	40.5
91	20.5
92	17.0
93	11.5
94	3.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042310464788290966
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0019588178142727303
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.83527125709092E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.018412887454163664
52-53	0.028990503651236406
54-55	0.049753972482527345
56-57	0.05641395305105463
58-59	0.03565048421976369
60-61	0.030165794339800045
62-63	0.030949321465509137
64-65	0.017629360328454573
66-67	0.036434011345472785
68-69	0.007443507694236375
70-71	0.013319961137054566
72-73	0.02154699595700003
74-75	0.010969379759927289
76	0.01645406963989093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	127628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.53170150750619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.8713526830395	11.868085373115617
2	20.725229039956886	12.655530134453254
3	16.103369517797113	14.749898141473658
4	10.806579926604563	13.197730905443947
5	6.567095234429132	10.025229573447833
6	3.5080965945543663	6.426489485065972
7	1.6064875407396002	3.4334158648572415
8	0.8006774963430595	1.9556837057698937
9	0.3849411040110863	1.0577616197072743
>10	0.5748453819898889	2.520606763406149
>50	0.017963918187184028	0.3823612373460369
>100	0.025662740267405757	2.0128811859466573
>500	0.0	0.0
>1k	0.005132548053481151	3.3260726486350958
>5k	0.0	0.0
>10k+	0.0025662740267405754	16.38825336133137
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	20916	16.38825336133137	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	2783	2.1805559908484033	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	1462	1.1455166577866926	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT	498	0.3901965086031278	Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	463	0.3627730592033096	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT	380	0.297740307769455	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT	364	0.2852038737581095	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	189	0.1480866267590184	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT	155	0.12144670448490927	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	135	0.10577616197072744	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT	133	0.10420910771930925	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009402325508509104	0.0
2	0.0	0.0	0.0	0.009402325508509104	0.0
3	0.0	0.0	0.0	0.009402325508509104	0.0
4	0.0	0.0	0.0	0.029774030776945497	0.0
5	0.0	0.0	0.0	0.03290813927978187	0.0
6	0.0	0.0	0.0	0.03290813927978187	0.0
7	0.0	0.0	0.0	0.03290813927978187	0.0
8	0.0	0.0	0.0	0.036042247782618234	0.0
9	0.0	0.0	0.0	0.036042247782618234	0.0
10	0.0	0.0	0.0	0.03760930203403642	0.0
11	0.0	0.0	0.0	0.03760930203403642	0.0
12	0.0	0.0	0.0	0.03760930203403642	0.0
13	0.0	0.0	0.0	0.0391763562854546	0.0
14	0.0	0.0	0.0	0.0391763562854546	0.0
15	0.0	0.0	0.0	0.04231046478829097	0.0
16	0.0	0.0	0.0	0.04309399191400006	0.0
17	0.0	0.0	0.0	0.043877519039709156	0.0
18	0.0	0.0	0.0	0.04701162754254552	0.0
19	0.0	0.0	0.0	0.0493622089196728	0.0
20	0.0	0.0	0.0	0.059548061553890995	0.0
21	0.0	0.0	0.0	0.06581627855956373	0.0
22	0.0	0.0	0.0	0.07521860406807283	0.0
23	0.0	0.0	0.0	0.07991976682232739	0.0
24	0.0	0.0	0.0	0.08462092957658195	0.0
25	0.0	0.0	0.0	0.09402325508509105	0.0
26	0.0	0.0	0.0	0.10264205346789106	0.0
27	0.0	0.0	0.0	0.16845833202745478	0.0
28	0.0	0.0	0.0	0.20214999843294576	0.0
29	0.0	0.0	0.0	0.22878992070705487	0.0
30	0.0	0.0	0.0	0.2577804243582913	0.0
31	0.0	0.0	0.0	0.297740307769455	0.0
32	0.0	0.0	0.0	0.3431848810605823	0.0
33	0.0	0.0	0.0	0.4144858495001097	0.0
34	0.0	0.0	0.0	0.47873507380825525	0.0
35	0.0	0.0	0.0	0.6189864293101828	0.0
36	0.0	0.0	0.0	0.8791174350456012	0.0
37	0.0	0.0	0.0	1.2489422383802928	0.0
38	0.0	0.0	0.0	1.6555928166233116	0.0
39	0.0	0.0	0.0	2.1484313786943305	0.0
40	0.0	0.0	0.0	2.6663428087880403	0.0
41	0.0	0.0	0.0	3.4420346632400416	0.0
42	0.0	0.0	0.0	4.292161594634406	0.0
43	0.0	0.0	0.0	5.269219920393644	0.0
44	0.0	0.0	0.0	6.061365844485536	0.0
45	0.0	0.0	0.0	6.795530761274955	0.0
46	0.0	0.0	0.0	7.408248973579465	0.0
47	0.0	0.0	0.0	8.096185789952049	0.0
48	0.0	0.0	0.0	8.798226094587395	0.0
49	0.0	0.0	0.0	9.280095276898486	0.0
50	0.0	0.0	0.0	9.876359419563105	0.0
51	0.0	0.0	0.0	10.57918325132416	0.0
52	0.0	0.0	0.0	11.441063089604162	0.0
53	0.0	0.0	0.0	12.244178393455982	0.0
54	0.0	0.0	0.0	12.860030714263328	0.0
55	0.0	0.0	0.0	13.529946406744601	0.0
56	0.0	0.0	0.0	14.113674115397876	0.0
57	0.0	0.0	0.0	14.728742909079513	0.0
58	0.0	0.0	0.0	15.293665966715768	0.0
59	0.0	0.0	0.0	15.789638637289624	0.0
60	0.0	0.0	0.0	16.237032626069514	0.0
61	0.0	0.0	0.0	16.71576769987777	0.0
62	0.0	0.0	0.0	17.185100448177515	0.0
63	0.0	0.0	0.0	17.687341335757043	0.0
64	0.0	0.0	0.0	18.25304792051901	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCGTA	55	3.45608E-11	57.269516	38
ATATCGT	25	2.3836212E-4	55.99687	37
ACTCGTA	50	9.713403E-10	55.99687	38
CTCGTAT	2995	0.0	53.636395	39
TATGCCG	3045	0.0	53.56022	43
CGTCTTC	3015	0.0	53.51276	48
TGCCGTC	3050	0.0	53.472412	45
TCGTATG	3035	0.0	53.39075	40
GTATGCC	3055	0.0	53.3849	42
ATGCCGT	3055	0.0	53.3849	44
GCCGTCT	3050	0.0	53.357666	46
CGTATGC	3045	0.0	53.33035	41
CTGCTTG	2990	0.0	53.278767	54
TGCTTGA	2980	0.0	53.222572	55
TCTCGTA	2870	0.0	53.16776	38
ATCTCGT	2865	0.0	53.138386	37
TCTGCTT	2995	0.0	53.072918	53
CCGTCTT	3055	0.0	53.04122	47
GATCTCG	2865	0.0	53.016228	36
GCTTGAA	3005	0.0	52.8963	56
>>END_MODULE