FastQCFastQC Report
Wed 25 May 2016
SRR1779858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269755
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCG150.002223380869.9974065
GCCGTAT601.8189894E-1258.33117354
AATGCGA252.3881841E-455.99792518
TACGAAT200.00694731952.4980538
GATCTCG1150.048.69384441
TCGGTGG1300.048.4597445
CTCGGTG1100.047.72550244
ATCTCGG1150.045.6504842
CGACGAG703.8198777E-1044.99833322
TGGTCGC1100.044.543849
GCGAACT404.5864188E-543.7483828
TAGATCT1950.043.07532539
AGATCTC1800.042.77619640
CGTATCA757.530616E-1041.99844756
ATTAACG503.4718396E-641.99844418
CGGTGGT1100.041.36210346
GTAGATC2000.040.2485138
ACGTGTA350.001254289839.9985234
CTCGACG350.001254289839.9985244
CGATCTA350.001254289839.9985225
Produced by FastQC (version 0.11.5)