##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779858_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269755
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.259539211506738	34.0	27.0	34.0	12.0	34.0
2	30.126040295823987	34.0	31.0	34.0	12.0	34.0
3	29.785694426423976	34.0	31.0	34.0	11.0	34.0
4	29.840640581268186	34.0	31.0	34.0	12.0	34.0
5	30.675857722748418	34.0	32.0	34.0	21.0	34.0
6	33.183881670404624	37.0	34.0	38.0	12.0	38.0
7	33.46951863728198	38.0	34.0	38.0	21.0	38.0
8	32.70945116865304	37.0	33.0	38.0	11.0	38.0
9	33.61601082463717	38.0	34.0	38.0	21.0	38.0
10-11	32.13226075512965	37.0	30.0	38.0	11.5	38.0
12-13	33.055704250152914	37.0	34.0	38.0	16.0	38.0
14-15	31.773310967359272	37.0	29.0	38.0	11.0	38.0
16-17	32.43154714463124	37.0	30.5	38.0	16.0	38.0
18-19	33.38262497451391	37.5	34.0	38.0	21.0	38.0
20-21	31.967442679468405	37.0	29.5	38.0	16.0	38.0
22-23	32.938086411743996	37.5	32.5	38.0	16.0	38.0
24-25	33.25645678486033	37.5	34.0	38.0	21.0	38.0
26-27	32.09889529387778	37.0	29.0	38.0	11.0	38.0
28-29	33.41306926655669	37.5	34.0	38.0	21.0	38.0
30-31	33.842468165557634	38.0	35.0	38.0	21.0	38.0
32-33	33.93740987192082	38.0	35.0	38.0	21.0	38.0
34-35	33.87004874793794	38.0	34.5	38.0	21.0	38.0
36-37	33.33192897258624	38.0	34.0	38.0	16.0	38.0
38-39	33.25396748901781	38.0	34.0	38.0	16.0	38.0
40-41	33.23512631832589	38.0	34.0	38.0	16.0	38.0
42-43	33.65251431854831	38.0	34.5	38.0	19.5	38.0
44-45	33.712928397990765	38.0	34.0	38.0	21.0	38.0
46-47	33.61798669162759	38.0	34.0	38.0	21.0	38.0
48-49	33.32406998943486	38.0	34.0	38.0	16.0	38.0
50-51	32.65274601026857	37.5	32.5	38.0	11.0	38.0
52-53	33.22803469815203	37.0	34.0	38.0	21.0	38.0
54-55	33.39646345758188	37.5	34.0	38.0	16.0	38.0
56-57	33.47718670645585	38.0	34.0	38.0	20.0	38.0
58-59	33.7550592204037	38.0	34.0	38.0	21.0	38.0
60-61	33.60820744749866	38.0	34.0	38.0	21.0	38.0
62-63	33.62306722766955	38.0	34.0	38.0	21.0	38.0
64-65	33.646036218049716	38.0	34.5	38.0	21.0	38.0
66-67	33.460979407239904	38.0	34.0	38.0	21.0	38.0
68-69	32.79895831402569	37.0	32.5	38.0	11.0	38.0
70-71	32.81206279772386	37.0	32.5	38.0	15.0	38.0
72-73	33.05038460825564	37.0	33.0	38.0	19.0	38.0
74-75	33.11214991381068	37.5	33.5	38.0	15.5	38.0
76	31.88677132953977	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	42.0
13	505.0
14	1923.0
15	3277.0
16	4294.0
17	4879.0
18	5056.0
19	4473.0
20	3903.0
21	3107.0
22	2475.0
23	2337.0
24	2376.0
25	2524.0
26	3025.0
27	3384.0
28	4089.0
29	5013.0
30	6130.0
31	7805.0
32	9926.0
33	13084.0
34	17623.0
35	26235.0
36	45477.0
37	86792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.3682368685427	18.63742015844124	9.955774356540983	32.038568616475075
2	22.180867824507423	20.450408704194547	31.44334674056088	25.92537673073715
3	18.747011176808588	22.72691887082723	29.952734889066	28.573335063298178
4	15.385813052584755	20.345498693258698	31.813312079479527	32.45537617467702
5	14.773776204333561	33.027376693666476	34.742266130377566	17.456580971622397
6	30.14050046339203	36.8533827618165	16.797775718257647	16.20834105653383
7	28.275005560910504	30.148661674204792	19.558092978423666	22.018239786461034
8	24.464314842000682	35.62806767798093	19.129706244346576	20.777911235671812
9	24.56502637832788	17.705548902787612	19.712377979379305	38.017046739505204
10-11	24.218164967562558	25.69471733086191	24.623169601482857	25.463948100092676
12-13	24.205712972350785	22.683773579310078	25.64442854521426	27.466084903124877
14-15	24.650236882492937	24.61576102671323	22.590211822623576	28.14379026817026
16-17	25.43484063257635	25.853926169769494	23.415408112576642	25.295825085077517
18-19	22.80630213160334	25.814828544949027	25.212418906394813	26.16645041705283
20-21	24.34931697280866	25.262738410780152	25.094066838427466	25.293877777983724
22-23	25.50898980537535	25.16237256719184	24.138276181649676	25.190361445783132
24-25	22.942010583972046	27.530560987590246	23.987988767273098	25.539439661164607
26-27	23.68032296151857	27.47675192351536	24.658623521103525	24.184301593862546
28-29	22.723590723642626	26.77268253259163	26.174142088941366	24.329584654824377
30-31	23.39876961744704	26.175007923900896	25.65230849646066	24.773913962191408
32-33	21.972159922892995	27.316083112453892	25.50295638635058	25.208800578302533
34-35	22.318608479024867	27.70845381615434	25.52141257766508	24.451525127155715
36-37	23.99099772162271	25.835018788733805	25.345232555768337	24.828750933875153
38-39	23.87041283340779	25.49905740295514	26.33024818941659	24.30028157422048
40-41	22.714997210416175	25.889932846098386	26.04507448466841	25.34999545881703
42-43	21.168029330481346	25.635997308654012	27.018185120860338	26.1777882400043
44-45	20.94041016667655	24.09877513494276	28.452162049943652	26.508652648437035
46-47	21.394214283198735	24.386794463773004	28.110235198822615	26.108756054205646
48-49	21.090843711597028	24.978452630076607	28.23270788809825	25.697995770228115
50-51	22.37806437741193	25.20398290287782	26.828988000133457	25.588964719576797
52-53	18.991389457878785	26.29802300469919	27.821968468176216	26.888619069245813
54-55	19.28776782387713	25.44880599532519	27.581202142034932	27.682224038762747
56-57	19.476910699621282	26.302022990125106	26.870009954601827	27.35105635565179
58-59	19.51527420153159	25.87577093980969	27.125402501487656	27.48355235717106
60-61	19.193376147605417	25.291443572668083	27.414977084971003	28.100203194755498
62-63	19.928817706595485	25.41689837986134	26.544322099877654	28.109961813665517
64-65	20.499372447381056	24.71778671368213	25.776561604903254	29.00627923403356
66-67	20.129141816847017	26.46016829592497	24.556967188041686	28.853722699186328
68-69	20.233582757655572	27.09797241565942	23.898025797530718	28.770419029154287
70-71	20.604290197197823	26.910765158690587	24.45316018824524	28.031784455866354
72-73	20.3637179230524	26.464732967057408	24.324354383077164	28.84719472681303
74-75	19.904607958358202	25.92565130186224	23.955525731382238	30.214215008397318
76	19.45222948826312	25.428177410027136	24.407965953408368	30.71162714830138
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.5
10	1.5
11	3.5
12	5.0
13	4.0
14	5.5
15	9.0
16	13.0
17	16.0
18	24.0
19	48.5
20	86.0
21	107.0
22	146.0
23	252.5
24	413.0
25	506.0
26	627.5
27	940.0
28	1246.5
29	1362.0
30	1657.0
31	2263.5
32	3273.5
33	3972.0
34	4374.5
35	5386.0
36	6804.0
37	7613.0
38	8218.0
39	9721.5
40	11495.5
41	12930.0
42	13489.0
43	14122.0
44	15296.0
45	16275.5
46	16714.0
47	16840.0
48	16697.0
49	15690.5
50	14953.0
51	14154.0
52	12470.0
53	10726.0
54	9867.0
55	9278.5
56	8123.5
57	6910.0
58	6263.0
59	5809.0
60	4908.0
61	4151.0
62	3841.0
63	3623.0
64	3232.5
65	2847.5
66	2476.0
67	2317.0
68	2169.5
69	1970.5
70	1749.5
71	1580.0
72	1530.0
73	1414.5
74	1267.5
75	1186.0
76	1165.0
77	990.0
78	759.5
79	683.0
80	645.5
81	573.0
82	504.0
83	470.0
84	393.0
85	296.0
86	221.5
87	167.0
88	153.5
89	114.0
90	74.0
91	53.5
92	47.0
93	38.0
94	27.5
95	24.0
96	22.0
97	17.5
98	11.0
99	11.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.414135048469907E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0018535337621174769
7	0.005560601286352431
8	0.0025949472669644677
9	0.00963837556301088
10-11	0.0018535337621174769
12-13	1.8535337621174768E-4
14-15	3.7070675242349536E-4
16-17	3.7070675242349536E-4
18-19	0.0018535337621174769
20-21	0.0
22-23	0.0018535337621174769
24-25	0.0027803006431762153
26-27	5.560601286352431E-4
28-29	0.005745954662564178
30-31	5.560601286352431E-4
32-33	0.0
34-35	0.0025949472669644677
36-37	0.01686715723526904
38-39	0.007970195177105151
40-41	5.560601286352431E-4
42-43	5.560601286352431E-4
44-45	0.004077774276658449
46-47	0.0016681803859057293
48-49	1.8535337621174768E-4
50-51	7.414135048469907E-4
52-53	0.010194435691646122
54-55	0.0042631276528701965
56-57	0.01130655594891661
58-59	0.013160089711034087
60-61	0.02335452540268021
62-63	0.009267668810587385
64-65	0.02094493151192749
66-67	0.019091397749810012
68-69	1.8535337621174768E-4
70-71	1.8535337621174768E-4
72-73	0.00481918778150544
74-75	0.00963837556301088
76	0.0025949472669644677
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	269755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.67955061387108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.03985331384452	47.51783531756477
2	10.007829279753375	12.345568251910585
3	5.517604749746968	10.209701442880608
4	3.0189546843073276	7.44831073006868
5	1.6058532290857883	4.9524152760922595
6	0.7917109149906093	2.929942407163048
7	0.45498618138813884	1.9644340242479144
8	0.3140450453099725	1.5496125813785508
9	0.22178122317150462	1.231142956183175
>10	1.021174340954471	9.633196909964694
>50	0.006207037447360914	0.21784010254571698
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001853533762117477	0.0
2	0.0	0.0	0.0	0.001853533762117477	0.0
3	0.0	0.0	0.0	0.001853533762117477	0.0
4	0.0	0.0	0.0	0.0029656540193879633	0.0
5	0.0	0.0	0.0	0.00481918778150544	0.0
6	0.0	0.0	0.0	0.00481918778150544	0.0
7	0.0	0.0	0.0	0.00481918778150544	0.0
8	0.0	0.0	0.0	0.005931308038775927	0.0
9	0.0	0.0	0.0	0.00889696205816389	0.0
10	0.0	0.0	0.0	0.01037978906785787	0.0
11	0.0	0.0	0.0	0.01037978906785787	0.0
12	0.0	0.0	0.0	0.010750495820281366	0.0
13	0.0	0.0	0.0	0.016681803859057293	0.0
14	0.0	0.0	0.0	0.016681803859057293	0.0
15	0.0	0.0	0.0	0.01779392411632778	0.0
16	0.0	0.0	0.0	0.01779392411632778	0.0
17	0.0	0.0	0.0	0.01779392411632778	0.0
18	0.0	0.0	0.0	0.018906044373598264	0.0
19	0.0	0.0	0.0	0.01927675112602176	0.0
20	0.0	0.0	0.0	0.01927675112602176	0.0
21	0.0	0.0	0.0	0.01927675112602176	0.0
22	0.0	0.0	0.0	0.024466645659950697	0.0
23	0.0	0.0	0.0	0.02817371318418565	0.0
24	0.0	0.0	0.0	0.0322514874608441	0.0
25	0.0	0.0	0.0	0.03595855498507905	0.0
26	0.0	0.0	0.0	0.04337269003354896	0.0
27	0.0	0.0	0.0	0.09527163537283832	0.0
28	0.0	0.0	0.0	0.14123927267335173	0.0
29	0.0	0.0	0.0	0.17868065466812477	0.0
30	0.0	0.0	0.0	0.24837352412374192	0.0
31	0.0	0.0	0.0	0.29693610869121984	0.0
32	0.0	0.0	0.0	0.3618097903653315	0.0
33	0.0	0.0	0.0	0.4289077125539842	0.0
34	0.0	0.0	0.0	0.515653092621082	0.0
35	0.0	0.0	0.0	0.6183388630423903	0.0
36	0.0	0.0	0.0	0.8563325980982743	0.0
37	0.0	0.0	0.0	1.2741191080795538	0.0
38	0.0	0.0	0.0	1.824247928676021	0.0
39	0.0	0.0	0.0	2.5575058849696948	0.0
40	0.0	0.0	0.0	3.3971566792089116	0.0
41	0.0	0.0	0.0	4.457748697892532	0.0
42	0.0	0.0	0.0	5.646975959667105	0.0
43	0.0	0.0	0.0	6.811736575781728	0.0
44	0.0	0.0	0.0	7.862690218902337	0.0
45	0.0	0.0	0.0	8.874719653018479	0.0
46	0.0	0.0	0.0	9.91084502604215	0.0
47	0.0	0.0	0.0	10.953272413857018	0.0
48	0.0	0.0	0.0	11.976423050545865	0.0
49	0.0	0.0	0.0	12.853515226779855	0.0
50	0.0	0.0	0.0	13.757668995940762	0.0
51	0.0	0.0	0.0	14.693703545810086	0.0
52	0.0	0.0	0.0	15.836592463531723	0.0
53	0.0	0.0	0.0	17.024336898296603	0.0
54	0.0	0.0	0.0	18.06009156456785	0.0
55	0.0	0.0	0.0	19.04209375173769	0.0
56	0.0	0.0	0.0	19.825397119608535	0.0
57	0.0	0.0	0.0	20.68506607847862	0.0
58	0.0	0.0	0.0	21.456506830271913	0.0
59	0.0	0.0	0.0	22.110804248299385	0.0
60	0.0	0.0	0.0	22.858519767937572	0.0
61	0.0	0.0	0.0	23.611795888862115	0.0
62	0.0	0.0	0.0	24.327259921039463	0.0
63	0.0	0.0	0.0	25.073863320420383	0.0
64	0.0	0.0	0.0	25.856795981538802	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCCG	15	0.0022233808	69.997406	5
GCCGTAT	60	1.8189894E-12	58.331173	54
AATGCGA	25	2.3881841E-4	55.997925	18
TACGAAT	20	0.006947319	52.49805	38
GATCTCG	115	0.0	48.693844	41
TCGGTGG	130	0.0	48.45974	45
CTCGGTG	110	0.0	47.725502	44
ATCTCGG	115	0.0	45.65048	42
CGACGAG	70	3.8198777E-10	44.998333	22
TGGTCGC	110	0.0	44.5438	49
GCGAACT	40	4.5864188E-5	43.74838	28
TAGATCT	195	0.0	43.075325	39
AGATCTC	180	0.0	42.776196	40
CGTATCA	75	7.530616E-10	41.998447	56
ATTAACG	50	3.4718396E-6	41.998444	18
CGGTGGT	110	0.0	41.362103	46
GTAGATC	200	0.0	40.24851	38
ACGTGTA	35	0.0012542898	39.99852	34
CTCGACG	35	0.0012542898	39.99852	44
CGATCTA	35	0.0012542898	39.99852	25
>>END_MODULE