FastQCFastQC Report
Wed 25 May 2016
SRR1779858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269755
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT220008.1555485533169No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG27711.0272284109655057No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC16230.601657059183333No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5150.19091397749810013No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT5080.18831903023113566RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT3910.14494634019758668No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT3620.13419584437730533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG150.00222473669.9866536
ATCTCGT31600.054.81549537
GATCTCG31650.054.61833636
TCTCGTA31850.054.49510238
CTCGTAT33500.054.4224639
GCCGTCT34050.054.18009646
CCGTCTT34000.054.15681547
CGTATGC34350.054.09448641
CTGCTTG33950.054.05039654
TCGTATG34400.054.01586540
TATGCCG34300.053.96930343
CGTCTTC34150.053.91893848
TCGATCT31500.053.87861334
TGCCGTC34300.053.87726645
CGATCTC31650.053.84439535
ATGCCGT34300.053.7652644
CTCGATC31250.053.74975233
CTTGAAA32900.053.74304657
GTCTTCT34400.053.63879449
GTATGCC34550.053.57878542