##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779858_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269755
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.52568071027414	34.0	34.0	34.0	34.0	34.0
2	33.602480028173716	34.0	34.0	34.0	34.0	34.0
3	33.75058108283442	34.0	34.0	34.0	34.0	34.0
4	33.534362662415894	34.0	34.0	34.0	34.0	34.0
5	33.58004114844952	34.0	34.0	34.0	34.0	34.0
6	37.24178235806565	38.0	38.0	38.0	36.0	38.0
7	37.32734147652499	38.0	38.0	38.0	37.0	38.0
8	37.2191358825601	38.0	38.0	38.0	36.0	38.0
9	37.114559507701436	38.0	38.0	38.0	36.0	38.0
10-11	37.07134066097014	38.0	37.5	38.0	35.5	38.0
12-13	37.24349131619432	38.0	38.0	38.0	36.5	38.0
14-15	37.27115530759393	38.0	38.0	38.0	36.0	38.0
16-17	37.205729272858704	38.0	38.0	38.0	36.0	38.0
18-19	37.06408963689273	38.0	38.0	38.0	35.5	38.0
20-21	37.06093121536209	38.0	38.0	38.0	35.5	38.0
22-23	37.04594354136161	38.0	38.0	38.0	35.5	38.0
24-25	37.251826657522564	38.0	38.0	38.0	36.0	38.0
26-27	37.15954662564179	38.0	38.0	38.0	36.0	38.0
28-29	36.62773627921633	38.0	37.0	38.0	34.0	38.0
30-31	36.910954384534115	38.0	37.0	38.0	35.5	38.0
32-33	36.96852699671925	38.0	37.5	38.0	36.0	38.0
34-35	37.04014383421994	38.0	38.0	38.0	36.0	38.0
36-37	37.06983373802154	38.0	38.0	38.0	36.0	38.0
38-39	36.49122166410261	38.0	37.0	38.0	34.0	38.0
40-41	36.80413894089081	38.0	37.0	38.0	35.0	38.0
42-43	37.15160052640359	38.0	38.0	38.0	36.0	38.0
44-45	36.91973828103279	38.0	37.5	38.0	35.5	38.0
46-47	37.06960019276751	38.0	38.0	38.0	36.0	38.0
48-49	36.71065967266594	38.0	37.0	38.0	34.5	38.0
50-51	36.90207595781357	38.0	37.5	38.0	35.5	38.0
52-53	36.83090396841578	38.0	37.5	38.0	35.0	38.0
54-55	36.832171785509075	38.0	37.0	38.0	35.0	38.0
56-57	36.69204834016051	38.0	37.0	38.0	34.5	38.0
58-59	36.814915386183756	38.0	37.0	38.0	35.0	38.0
60-61	36.48312913569721	38.0	37.0	38.0	34.0	38.0
62-63	36.27468999647829	38.0	37.0	38.0	34.0	38.0
64-65	36.64341346777631	38.0	37.0	38.0	34.0	38.0
66-67	36.43126911456692	38.0	37.0	38.0	34.0	38.0
68-69	36.54042371781802	38.0	37.0	38.0	34.0	38.0
70-71	35.88383533206057	38.0	37.0	38.0	31.5	38.0
72-73	34.73281681525829	38.0	37.0	38.0	23.0	38.0
74-75	34.567270300828525	38.0	37.0	38.0	23.0	38.0
76	34.23473151563456	38.0	36.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	5.0
22	18.0
23	20.0
24	56.0
25	129.0
26	233.0
27	461.0
28	801.0
29	1412.0
30	2324.0
31	3693.0
32	5746.0
33	9115.0
34	15712.0
35	29412.0
36	42868.0
37	157750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.32495688136348	16.909181951373306	11.058029339218486	33.70783182804473
2	19.649311412207375	18.175752071323977	37.89735130025393	24.277585216214714
3	18.470093232748237	18.979444310578117	35.96967618765176	26.58078626902189
4	13.133398824859595	23.66740190172564	33.88667494578414	29.31252432763063
5	12.9962373264629	31.732498007451206	40.74400845211395	14.527256213971937
6	27.679190376452706	41.63852384571185	17.038794461641118	13.643491316194325
7	26.294971362903375	27.218401883190303	19.774610294526514	26.712016459379807
8	23.530240403328946	39.78906785787103	17.214509462289858	19.466182276510168
9	22.219421326759466	16.69663212915423	18.288817630813146	42.79512891327315
10-11	23.128023576949456	27.733313562306538	27.525532427573168	21.61313043317084
12-13	23.434968767956107	20.450408704194547	28.258234323738208	27.856388204111138
14-15	21.218883801968452	21.00776630646327	21.36179125502771	36.41155863654056
16-17	26.53111156419714	28.34868677132954	21.975125576912383	23.145076087560934
18-19	26.574113547478266	24.218272135826954	23.09892309688421	26.10869121981057
20-21	20.956238067876406	23.60475246056607	22.894663676298862	32.544345795258664
22-23	21.388667494578414	28.446368000593132	22.8826157068451	27.282348797983357
24-25	26.33284214233418	28.241628900566262	22.965866859435966	22.4596620976636
26-27	26.131675038460827	29.32438694370818	23.11838520138644	21.42555281644455
28-29	21.01740468202628	29.052102834053123	23.78732553613464	26.143166947785957
30-31	20.655038831532316	24.560805175066264	28.567589108635616	26.216566884765808
32-33	20.419454690367186	28.57648607069378	23.792886137420993	27.211173101518042
34-35	20.794980630572184	23.4648106615262	28.90789790736038	26.832310800541233
36-37	26.070137717558527	27.469370354581006	23.799373505588402	22.66111842227206
38-39	20.939556264017348	24.342458897888825	28.73996774851254	25.978017089581286
40-41	20.382198661748625	22.240180904895183	29.910474319289726	27.467146114066466
42-43	25.454070275009315	21.759785286251017	29.737780092639788	23.04836434609988
44-45	20.173490760134197	26.664195288317178	30.912680024466642	22.249633927081984
46-47	24.63698541268929	21.488572964356546	26.83509110118441	27.039350521769755
48-49	23.956738521992175	21.252803469815202	26.363922818854146	28.426535189338477
50-51	19.57966119290648	21.756620975785193	31.288304889307444	27.37541294200088
52-53	17.858434086766923	22.17175022666046	36.162031174388574	23.807784512184043
54-55	17.260569152427966	22.291185295350278	32.11078926970022	28.33745628252154
56-57	22.459182992233455	22.10423636404098	26.768613371510803	28.66796727221476
58-59	17.41133673499659	22.03714141623899	35.25912960930315	25.29239223946127
60-61	22.607218809621372	26.972731537190757	26.29746301504985	24.122586638138024
62-63	17.858189059006317	33.700307397945	23.792378459153895	24.649125083894795
64-65	17.675803336064572	34.21338504146909	22.657469440417618	25.453342182048726
66-67	17.62450331248621	34.09422553831378	21.84331096578488	26.437960183415132
68-69	17.73869747283853	34.56852142609541	21.64168175894739	26.05109934211868
70-71	18.194440839843242	33.674184422932186	21.635454939807282	26.495919797417294
72-73	18.56219595396298	31.677889178927387	21.478071606549594	28.281843260560034
74-75	18.39751004730762	31.143687621420412	21.62618083670715	28.832621494564815
76	18.168875706738344	31.251830568171286	21.786634535174716	28.792659189915653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.5
15	13.5
16	25.0
17	32.0
18	35.5
19	64.5
20	121.0
21	152.0
22	181.5
23	291.0
24	439.5
25	508.0
26	689.0
27	951.5
28	1290.5
29	1548.0
30	1776.0
31	2247.5
32	3100.5
33	3710.0
34	4198.5
35	5220.0
36	6323.0
37	6893.0
38	7664.0
39	9368.5
40	14085.5
41	20358.0
42	22847.0
43	21841.5
44	19844.5
45	17596.0
46	16339.0
47	15926.0
48	14910.0
49	13614.0
50	12921.0
51	12087.5
52	10270.0
53	8882.5
54	8479.0
55	7735.0
56	6595.0
57	5565.5
58	4932.0
59	4488.5
60	3798.5
61	3261.5
62	2971.0
63	2815.5
64	2545.0
65	2423.5
66	2195.0
67	1973.0
68	1915.5
69	1797.5
70	1665.0
71	1593.0
72	1507.5
73	1329.0
74	1178.0
75	1120.0
76	1083.5
77	946.0
78	767.0
79	689.0
80	631.5
81	551.5
82	486.5
83	444.0
84	375.5
85	269.0
86	173.0
87	115.0
88	97.5
89	65.0
90	37.0
91	17.0
92	10.0
93	10.0
94	6.5
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05560601286352431
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.267668810587384E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.8535337621174768E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.016311097106633796
52-53	0.02910048006524439
54-55	0.04670905080536042
56-57	0.05078682508201887
58-59	0.030768660451150117
60-61	0.03132472057978536
62-63	0.026690886174491668
64-65	0.013345443087245834
66-67	0.03466108135159682
68-69	0.009453022186799132
70-71	0.014086856592092824
72-73	0.02187169839298623
74-75	0.011491909325128356
76	0.011121202572704861
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	269755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.06122222016274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.73201002753462	9.940130859483604
2	17.746818449567805	9.605011955292765
3	14.950889738215592	12.137680487850085
4	10.656310360415896	11.534911308409484
5	6.942560856998041	9.393709106411373
6	4.042521130426445	6.563733758410409
7	2.294551980164112	4.346536672165484
8	1.3534431978520254	2.9300661711553078
9	0.9931642899217796	2.4188615595633074
>10	4.200057535034726	19.050620007043428
>50	0.06712420718092028	1.171062630905822
>100	0.013698817792024549	0.7440084521139553
>500	0.0027397635584049096	0.3792330077292358
>1k	0.0027397635584049096	1.6288854701488387
>5k	0.0	0.0
>10k+	0.0013698817792024548	8.1555485533169
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	22000	8.1555485533169	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	2771	1.0272284109655057	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	1623	0.601657059183333	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	515	0.19091397749810013	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	508	0.18831903023113566	RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	391	0.14494634019758668	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	362	0.13419584437730533	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004448481029081945	0.0
2	0.0	0.0	0.0	0.004448481029081945	0.0
3	0.0	0.0	0.0	0.004448481029081945	0.0
4	0.0	0.0	0.0	0.005189894533928935	0.0
5	0.0	0.0	0.0	0.007414135048469908	0.0
6	0.0	0.0	0.0	0.007414135048469908	0.0
7	0.0	0.0	0.0	0.007414135048469908	0.0
8	0.0	0.0	0.0	0.00963837556301088	0.0
9	0.0	0.0	0.0	0.012233322829975348	0.0
10	0.0	0.0	0.0	0.015198976849363312	0.0
11	0.0	0.0	0.0	0.015198976849363312	0.0
12	0.0	0.0	0.0	0.015569683601786807	0.0
13	0.0	0.0	0.0	0.021500991640562732	0.0
14	0.0	0.0	0.0	0.021500991640562732	0.0
15	0.0	0.0	0.0	0.023725232155103707	0.0
16	0.0	0.0	0.0	0.023725232155103707	0.0
17	0.0	0.0	0.0	0.023725232155103707	0.0
18	0.0	0.0	0.0	0.025208059164797687	0.0
19	0.0	0.0	0.0	0.025949472669644678	0.0
20	0.0	0.0	0.0	0.026320179422068175	0.0
21	0.0	0.0	0.0	0.026320179422068175	0.0
22	0.0	0.0	0.0	0.026690886174491668	0.0
23	0.0	0.0	0.0	0.0322514874608441	0.0
24	0.0	0.0	0.0	0.03373431447053808	0.0
25	0.0	0.0	0.0	0.03855350225204352	0.0
26	0.0	0.0	0.0	0.04633834405293692	0.0
27	0.0	0.0	0.0	0.1052807176882727	0.0
28	0.0	0.0	0.0	0.15532612926544456	0.0
29	0.0	0.0	0.0	0.19795740579414653	0.0
30	0.0	0.0	0.0	0.27024522251672817	0.0
31	0.0	0.0	0.0	0.3236269948657115	0.0
32	0.0	0.0	0.0	0.39294915756890514	0.0
33	0.0	0.0	0.0	0.4685733350632982	0.0
34	0.0	0.0	0.0	0.5608793164167485	0.0
35	0.0	0.0	0.0	0.6713499286389502	0.0
36	0.0	0.0	0.0	0.9326981890975145	0.0
37	0.0	0.0	0.0	1.3920038553502252	0.0
38	0.0	0.0	0.0	1.9721599228929956	0.0
39	0.0	0.0	0.0	2.740264313914478	0.0
40	0.0	0.0	0.0	3.632926173750255	0.0
41	0.0	0.0	0.0	4.767659542918574	0.0
42	0.0	0.0	0.0	6.03881299697874	0.0
43	0.0	0.0	0.0	7.285870512131378	0.0
44	0.0	0.0	0.0	8.406146317955182	0.0
45	0.0	0.0	0.0	9.48230802024059	0.0
46	0.0	0.0	0.0	10.594798984263498	0.0
47	0.0	0.0	0.0	11.699505106485514	0.0
48	0.0	0.0	0.0	12.789753665363014	0.0
49	0.0	0.0	0.0	13.729865989509	0.0
50	0.0	0.0	0.0	14.676651035198606	0.0
51	0.0	0.0	0.0	15.654204741339363	0.0
52	0.0	0.0	0.0	16.820818891216103	0.0
53	0.0	0.0	0.0	18.038590572927287	0.0
54	0.0	0.0	0.0	19.088432095790626	0.0
55	0.0	0.0	0.0	20.07858983151378	0.0
56	0.0	0.0	0.0	20.86745380067098	0.0
57	0.0	0.0	0.0	21.755296472725252	0.0
58	0.0	0.0	0.0	22.53674630683398	0.0
59	0.0	0.0	0.0	23.206613408463234	0.0
60	0.0	0.0	0.0	23.973234972475023	0.0
61	0.0	0.0	0.0	24.745787844525587	0.0
62	0.0	0.0	0.0	25.47756297380957	0.0
63	0.0	0.0	0.0	26.25827139441345	0.0
64	0.0	0.0	0.0	27.080128264536338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATCG	15	0.002224736	69.98665	36
ATCTCGT	3160	0.0	54.815495	37
GATCTCG	3165	0.0	54.618336	36
TCTCGTA	3185	0.0	54.495102	38
CTCGTAT	3350	0.0	54.42246	39
GCCGTCT	3405	0.0	54.180096	46
CCGTCTT	3400	0.0	54.156815	47
CGTATGC	3435	0.0	54.094486	41
CTGCTTG	3395	0.0	54.050396	54
TCGTATG	3440	0.0	54.015865	40
TATGCCG	3430	0.0	53.969303	43
CGTCTTC	3415	0.0	53.918938	48
TCGATCT	3150	0.0	53.878613	34
TGCCGTC	3430	0.0	53.877266	45
CGATCTC	3165	0.0	53.844395	35
ATGCCGT	3430	0.0	53.76526	44
CTCGATC	3125	0.0	53.749752	33
CTTGAAA	3290	0.0	53.743046	57
GTCTTCT	3440	0.0	53.638794	49
GTATGCC	3455	0.0	53.578785	42
>>END_MODULE