FastQCFastQC Report
Wed 25 May 2016
SRR1779857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130207
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCG207.9022815E-570.0000151
CGTATCC150.00222046470.056
CGACGTA150.00222046470.022
GTTACGA150.00222046470.054
ACGTGTA150.00222046470.034
GAGCATG150.00222046470.01
CGATAAG150.00222046470.016
ACGGTGC150.00222046470.015
TCGGCGG401.0573785E-861.25000445
TCGCCGT850.057.6470652
GCGCCGT252.3831439E-455.99999652
TGCATTC252.3831439E-455.99999613
TACGCTC252.3831439E-455.99999614
CTCTCGG509.713403E-1055.99999642
CTTCCGA700.055.018
ACAACGG200.006938268452.5000048
TTCAGCA200.006938268452.50000414
GACGTAC200.006938268452.50000423
TTTCCAT200.006938268452.50000462
GCTTGCC200.006938268452.50000411
Produced by FastQC (version 0.11.5)