##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779857_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130207
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.87599745021389	32.0	21.0	34.0	12.0	34.0
2	27.35703149600252	34.0	21.0	34.0	12.0	34.0
3	27.01227276567312	34.0	21.0	34.0	11.0	34.0
4	27.54555438647692	34.0	23.0	34.0	12.0	34.0
5	27.60216424616188	34.0	23.0	34.0	11.0	34.0
6	29.96000215042202	36.0	23.0	38.0	11.0	38.0
7	30.360548972021473	37.0	24.0	38.0	12.0	38.0
8	29.650779143978433	36.0	21.0	38.0	11.0	38.0
9	30.45208014930073	37.0	25.0	38.0	11.0	38.0
10-11	29.237172348644847	35.0	21.0	38.0	11.0	38.0
12-13	29.780553272865514	36.0	22.0	38.0	11.0	38.0
14-15	28.75369987788675	34.5	21.0	38.0	11.0	38.0
16-17	29.17802422296804	35.0	22.0	38.0	11.0	38.0
18-19	29.719008962651777	36.5	21.5	38.0	11.0	38.0
20-21	28.886976122635495	35.0	22.0	38.0	11.0	38.0
22-23	29.52611994746826	36.5	22.0	38.0	11.0	38.0
24-25	29.794723017963705	36.5	22.0	38.0	11.0	38.0
26-27	28.838902670363346	34.5	21.0	38.0	11.0	38.0
28-29	29.656550723079405	36.5	21.0	38.0	11.0	38.0
30-31	29.924305144884684	37.0	21.5	38.0	11.0	38.0
32-33	30.072960747118053	37.0	22.5	38.0	11.0	38.0
34-35	30.050458116691114	37.0	23.0	38.0	11.0	38.0
36-37	29.652415000729608	36.5	21.5	38.0	11.0	38.0
38-39	29.56199359481441	36.5	21.0	38.0	11.0	38.0
40-41	29.5532651854355	36.5	21.0	38.0	11.0	38.0
42-43	29.909770596050905	37.0	22.5	38.0	11.0	38.0
44-45	29.95675347715561	37.0	22.5	38.0	11.0	38.0
46-47	29.894767562419837	37.0	22.0	38.0	11.0	38.0
48-49	29.589806999623676	36.5	21.5	38.0	11.0	38.0
50-51	29.078966568617663	36.0	21.0	38.0	11.0	38.0
52-53	29.592475826952466	36.5	21.5	38.0	11.0	38.0
54-55	29.665021849823745	36.5	21.0	38.0	11.0	38.0
56-57	29.745374672636647	37.0	22.0	38.0	11.0	38.0
58-59	29.85384810340458	37.0	21.5	38.0	11.0	38.0
60-61	29.876512015483037	37.0	22.5	38.0	11.0	38.0
62-63	29.789454484013916	37.0	21.5	38.0	11.0	38.0
64-65	29.758238804365355	37.0	21.5	38.0	11.0	38.0
66-67	29.813900174337785	37.0	22.5	38.0	11.0	38.0
68-69	29.358636632439115	36.0	21.5	38.0	11.0	38.0
70-71	29.3888615819426	36.0	21.5	38.0	11.0	38.0
72-73	29.52316311719032	36.0	21.5	38.0	11.0	38.0
74-75	29.603519780042546	36.5	22.0	38.0	11.0	38.0
76	28.719999692796854	34.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	53.0
13	639.0
14	2400.0
15	4302.0
16	5678.0
17	6732.0
18	7000.0
19	6106.0
20	4612.0
21	3259.0
22	2314.0
23	1670.0
24	1391.0
25	1167.0
26	1106.0
27	1260.0
28	1413.0
29	1648.0
30	1921.0
31	2416.0
32	3191.0
33	4312.0
34	5820.0
35	8982.0
36	16355.0
37	34456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52743708095571	21.266905773115116	14.313362568832705	31.892294577096468
2	16.303270945494482	28.546084311903353	31.63117190320029	23.519472839401875
3	14.513812621441243	29.25341955501624	31.740997027809563	24.491770795732947
4	11.156082238282119	27.87100539909529	29.077545754068524	31.89536660855407
5	11.262067323569394	35.52113173638898	35.47505126452495	17.741749675516676
6	21.0968941046358	41.76830204909219	19.29741021781205	17.837393628459957
7	21.30414746543779	33.528417818740394	19.43625192012289	25.731182795698928
8	18.57128593482385	40.55345197041497	19.217210313284845	21.658051781476335
9	19.94915553643981	20.371579327347717	19.481417193416334	40.19784794279614
10-11	19.54025636890087	30.54691520164666	24.73675721757563	25.176071211876838
12-13	19.26086923130093	25.138049413625996	26.229004585006948	29.372076770066123
14-15	24.541401542933723	27.7570628194445	21.113001271057897	26.58853436656388
16-17	25.38083736219006	26.624246869395922	21.42097360730839	26.57394216110563
18-19	19.955761387986453	26.76282420527945	25.144581922075528	28.13683248465857
20-21	23.850868232890704	26.212876419854542	23.458032210249833	26.478223137004925
22-23	25.26256772999397	25.897338417643017	22.245774915613513	26.5943189367495
24-25	19.580427636631747	30.539768363491138	22.195938680839298	27.68386531903782
26-27	23.169617142198838	30.140163588187857	22.833224530547984	23.85699473906532
28-29	20.32988344630273	29.758251886556963	25.266230150348505	24.645634516791798
30-31	21.471195865045658	27.87561344628169	25.24518651070987	25.408004177962784
32-33	19.037452047324827	29.960485843640676	24.766428711316255	26.23563339771824
34-35	19.331203342447235	30.82892998678996	24.46199809529661	25.377868575466188
36-37	23.284851231511958	26.427290289625187	24.395958075208068	25.891900403654784
38-39	23.145605714395437	25.359549914552893	26.42447051594693	25.070373855104744
40-41	20.500975377478763	28.641153249466228	25.284549099119857	25.57332227393515
42-43	17.886272522003594	27.825138626484186	26.402393130884903	27.886195720627317
44-45	17.290441007050582	25.968495107602035	28.489577733060933	28.251486152286446
46-47	19.46798665166451	26.479702926572635	26.752353047345117	27.299957374417737
48-49	19.614996179146203	27.41875405605711	26.538613663680383	26.427636101116303
50-51	23.754464114281326	26.42448446680235	23.86160285703314	25.959448561883185
52-53	15.94658740034103	28.857320613853172	26.574957371307434	28.621134614498363
54-55	17.383657073511678	26.586250067205853	26.517892117329893	29.512200741952583
56-57	18.508362616894214	28.314227010004416	24.971868579219233	28.205541793882137
58-59	17.79616102495564	28.46241291640743	25.227550291494804	28.51387576714212
60-61	16.280838413053246	27.047924132606617	25.41549996351052	31.255737490829617
62-63	17.49077320716022	27.12965132132282	25.267394568769852	30.11218090274711
64-65	18.136774788269864	26.55220756275086	24.66516871195099	30.645848937028287
66-67	17.21346105098208	30.999823325626235	21.954863538250002	29.831852085141687
68-69	17.806330713136443	32.73415689692911	20.919462546032648	28.540049843901805
70-71	17.113650676620125	32.172096523969714	22.358800669708	28.355452129702165
72-73	16.83646627368906	30.131525892359978	22.157024634703635	30.874983199247325
74-75	16.52457429894698	28.785609499450832	21.62569030008372	33.06412590151847
76	15.775366158997873	28.365705859311994	22.553243780865266	33.305684200824864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	3.5
16	10.5
17	14.0
18	11.0
19	12.5
20	18.0
21	19.0
22	32.0
23	84.5
24	152.5
25	181.0
26	216.0
27	295.5
28	413.5
29	487.0
30	645.0
31	927.0
32	1290.5
33	1530.0
34	1777.5
35	2356.5
36	3057.5
37	3427.0
38	3933.0
39	5009.0
40	6240.5
41	7298.5
42	7695.0
43	8004.5
44	8725.0
45	9250.5
46	9365.0
47	9496.0
48	9267.5
49	8684.0
50	8460.0
51	7897.0
52	6749.5
53	5714.0
54	5263.0
55	4776.5
56	3879.0
57	3055.5
58	2643.0
59	2388.0
60	1862.5
61	1403.5
62	1215.0
63	1081.0
64	857.5
65	707.5
66	573.5
67	500.0
68	458.0
69	360.0
70	263.5
71	223.0
72	206.5
73	168.0
74	133.5
75	121.0
76	103.5
77	75.5
78	63.0
79	61.0
80	48.0
81	34.0
82	28.5
83	24.0
84	20.5
85	13.0
86	10.5
87	12.0
88	9.5
89	7.0
90	6.5
91	5.5
92	5.0
93	5.5
94	5.5
95	4.0
96	3.0
97	2.0
98	5.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0023040235932015943
7	0.00537605505080372
8	0.0046080471864031886
9	0.0046080471864031886
10-11	0.0030720314576021257
12-13	0.0
14-15	3.840039322002657E-4
16-17	3.840039322002657E-4
18-19	0.0030720314576021257
20-21	0.0
22-23	0.0019200196610013286
24-25	0.0023040235932015943
26-27	0.0015360157288010629
28-29	0.0072960747118050485
30-31	0.0
32-33	3.840039322002657E-4
34-35	0.0023040235932015943
36-37	0.016512169084611426
38-39	0.0072960747118050485
40-41	7.680078644005314E-4
42-43	7.680078644005314E-4
44-45	0.003840039322002657
46-47	0.00268802752540186
48-49	3.840039322002657E-4
50-51	0.0015360157288010629
52-53	0.009984102237206909
54-55	0.007680078644005314
56-57	0.011136114033807706
58-59	0.012288125830408503
60-61	0.024960255593017275
62-63	0.011904121898208237
64-65	0.022656231999815677
66-67	0.018432188745612754
68-69	3.840039322002657E-4
70-71	7.680078644005314E-4
72-73	0.003456035389802392
74-75	0.007680078644005314
76	0.0030720314576021257
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	130207.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.16991405991998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.98274036186656	58.61436021104856
2	4.731754579662468	6.451266060964464
3	3.018183456884703	6.172479206187071
4	2.0707059327189565	5.646393819072707
5	1.2741939118091077	4.343084473185006
6	0.7976386291431018	3.2624974079734574
7	0.5171131790631126	2.4676092683189075
8	0.358261418174444	1.953812007034952
9	0.30418422297830155	1.8662591104932915
>10	0.9440976994659878	9.177693979586351
>50	0.0011266082332529687	0.04454445613523082
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015360157288010629	0.0
2	0.0	0.0	0.0	0.0023040235932015943	0.0
3	0.0	0.0	0.0	0.0023040235932015943	0.0
4	0.0	0.0	0.0	0.006144062915204251	0.0
5	0.0	0.0	0.0	0.011520117966007971	0.0
6	0.0	0.0	0.0	0.011520117966007971	0.0
7	0.0	0.0	0.0	0.011520117966007971	0.0
8	0.0	0.0	0.0	0.011520117966007971	0.0
9	0.0	0.0	0.0	0.011520117966007971	0.0
10	0.0	0.0	0.0	0.013056133694809034	0.0
11	0.0	0.0	0.0	0.015360157288010629	0.0
12	0.0	0.0	0.0	0.015360157288010629	0.0
13	0.0	0.0	0.0	0.01689617301681169	0.0
14	0.0	0.0	0.0	0.019968204474413817	0.0
15	0.0	0.0	0.0	0.023040235932015943	0.0
16	0.0	0.0	0.0	0.023040235932015943	0.0
17	0.0	0.0	0.0	0.023040235932015943	0.0
18	0.0	0.0	0.0	0.023040235932015943	0.0
19	0.0	0.0	0.0	0.02611226738961807	0.0
20	0.0	0.0	0.0	0.03302433816922285	0.0
21	0.0	0.0	0.0	0.034560353898023914	0.0
22	0.0	0.0	0.0	0.0537605505080372	0.0
23	0.0	0.0	0.0	0.0537605505080372	0.0
24	0.0	0.0	0.0	0.07142473138924943	0.0
25	0.0	0.0	0.0	0.07372875498245102	0.0
26	0.0	0.0	0.0	0.07449676284685156	0.0
27	0.0	0.0	0.0	0.08985692013486218	0.0
28	0.0	0.0	0.0	0.10521707742287281	0.0
29	0.0	0.0	0.0	0.14822551782930257	0.0
30	0.0	0.0	0.0	0.1681937223037164	0.0
31	0.0	0.0	0.0	0.1881619267781302	0.0
32	0.0	0.0	0.0	0.22809833572695784	0.0
33	0.0	0.0	0.0	0.27033876826898706	0.0
34	0.0	0.0	0.0	0.31795525586182005	0.0
35	0.0	0.0	0.0	0.4170282703694886	0.0
36	0.0	0.0	0.0	0.6005821499612156	0.0
37	0.0	0.0	0.0	0.9193054136874361	0.0
38	0.0	0.0	0.0	1.3609099357177417	0.0
39	0.0	0.0	0.0	1.8447548902900766	0.0
40	0.0	0.0	0.0	2.401560591980462	0.0
41	0.0	0.0	0.0	3.068191418280123	0.0
42	0.0	0.0	0.0	3.65648544241093	0.0
43	0.0	0.0	0.0	4.326956308032594	0.0
44	0.0	0.0	0.0	4.988979087145853	0.0
45	0.0	0.0	0.0	5.855291958189651	0.0
46	0.0	0.0	0.0	6.786117489843096	0.0
47	0.0	0.0	0.0	7.800655878716198	0.0
48	0.0	0.0	0.0	8.734553441827245	0.0
49	0.0	0.0	0.0	9.592418226362637	0.0
50	0.0	0.0	0.0	10.398058476118795	0.0
51	0.0	0.0	0.0	11.16990637984133	0.0
52	0.0	0.0	0.0	11.93714623637746	0.0
53	0.0	0.0	0.0	12.708994140099994	0.0
54	0.0	0.0	0.0	13.361032816976046	0.0
55	0.0	0.0	0.0	14.006927430936893	0.0
56	0.0	0.0	0.0	14.648213997711336	0.0
57	0.0	0.0	0.0	15.24572411621495	0.0
58	0.0	0.0	0.0	15.91005091892141	0.0
59	0.0	0.0	0.0	16.53290529695024	0.0
60	0.0	0.0	0.0	17.28478499619836	0.0
61	0.0	0.0	0.0	17.910711405684793	0.0
62	0.0	0.0	0.0	18.42374065910435	0.0
63	0.0	0.0	0.0	18.976706321472733	0.0
64	0.0	0.0	0.0	19.45363920526546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCCG	20	7.9022815E-5	70.00001	51
CGTATCC	15	0.002220464	70.0	56
CGACGTA	15	0.002220464	70.0	22
GTTACGA	15	0.002220464	70.0	54
ACGTGTA	15	0.002220464	70.0	34
GAGCATG	15	0.002220464	70.0	1
CGATAAG	15	0.002220464	70.0	16
ACGGTGC	15	0.002220464	70.0	15
TCGGCGG	40	1.0573785E-8	61.250004	45
TCGCCGT	85	0.0	57.64706	52
GCGCCGT	25	2.3831439E-4	55.999996	52
TGCATTC	25	2.3831439E-4	55.999996	13
TACGCTC	25	2.3831439E-4	55.999996	14
CTCTCGG	50	9.713403E-10	55.999996	42
CTTCCGA	70	0.0	55.0	18
ACAACGG	20	0.0069382684	52.500004	8
TTCAGCA	20	0.0069382684	52.500004	14
GACGTAC	20	0.0069382684	52.500004	23
TTTCCAT	20	0.0069382684	52.500004	62
GCTTGCC	20	0.0069382684	52.500004	11
>>END_MODULE