FastQCFastQC Report
Wed 25 May 2016
SRR1779857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130207
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3150124.19301573648114No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG38572.96220633299285No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC23921.8370748116460711No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT7230.5552696859615842RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6800.5222453477923614No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT3960.3041311143026105No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT3630.27878685477739296No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2990.2296343514557589No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2440.18739391891372967No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2290.1758738009477217No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2120.16281766725291266No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC1950.14976153355810365No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGT1500.11520117966007971No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT1450.11136114033807706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGT150.002220512669.9996234
GAATGCA150.002220512669.9996253
TCTTCCG150.002220512669.9996250
CGTGATA150.002220512669.9996224
CCGGATC150.002220512669.9996233
TTCGTGA150.002220512669.9996222
CCGCTTG150.002220512669.9996254
CGGTGGA150.002220512669.9996237
TGCTCGA150.002220512669.9996255
CTCGAAA150.002220512669.9996257
GTCTTCC150.002220512669.9996249
GGTTCAC150.002220512669.9996233
GTGGTTC150.002220512669.9996231
TCGGTGG150.002220512669.9996236
CTGGATA150.002220512669.9996239
CGCTTGA150.002220512669.9996255
TCTGCCG207.902499E-569.9996143
CGTGCGA207.902499E-569.9996110
CCGTGCG207.902499E-569.999619
AGTCTTC207.902499E-569.9996148
Produced by FastQC (version 0.11.5)