##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779857_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130207
Sequences flagged as poor quality	0
Sequence length	76
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.54229035305322	34.0	34.0	34.0	34.0	34.0
2	33.620066509481056	34.0	34.0	34.0	34.0	34.0
3	33.75617286321012	34.0	34.0	34.0	34.0	34.0
4	33.54929458477655	34.0	34.0	34.0	34.0	34.0
5	33.60588908430422	34.0	34.0	34.0	34.0	34.0
6	37.320727764252304	38.0	38.0	38.0	37.0	38.0
7	37.37219965132443	38.0	38.0	38.0	37.0	38.0
8	37.28503843879361	38.0	38.0	38.0	37.0	38.0
9	37.17971384026973	38.0	38.0	38.0	36.0	38.0
10-11	37.1260262505088	38.0	38.0	38.0	35.5	38.0
12-13	37.28586788728717	38.0	38.0	38.0	36.5	38.0
14-15	37.31548227053845	38.0	38.0	38.0	36.5	38.0
16-17	37.18131897670632	38.0	38.0	38.0	36.0	38.0
18-19	37.006190143387066	38.0	38.0	38.0	35.5	38.0
20-21	37.066709163101834	38.0	38.0	38.0	35.5	38.0
22-23	37.10325097729	38.0	38.0	38.0	36.0	38.0
24-25	37.2635687789443	38.0	38.0	38.0	36.0	38.0
26-27	37.12527744284101	38.0	38.0	38.0	36.0	38.0
28-29	36.574316280998715	38.0	37.0	38.0	34.0	38.0
30-31	36.78100639750551	38.0	37.0	38.0	35.0	38.0
32-33	36.77801116683435	38.0	37.0	38.0	34.5	38.0
34-35	36.90867234480481	38.0	37.5	38.0	36.0	38.0
36-37	36.84893285307241	38.0	37.5	38.0	35.0	38.0
38-39	35.645245647315434	38.0	36.5	38.0	32.5	38.0
40-41	36.55124532475213	38.0	37.0	38.0	34.5	38.0
42-43	37.12835331433794	38.0	37.5	38.0	36.0	38.0
44-45	36.930034483553115	38.0	37.5	38.0	35.0	38.0
46-47	37.08457686606711	38.0	38.0	38.0	36.0	38.0
48-49	36.66746795487185	38.0	37.0	38.0	34.0	38.0
50-51	36.793574846206425	38.0	37.0	38.0	34.5	38.0
52-53	36.744733386069875	38.0	37.0	38.0	34.5	38.0
54-55	36.75015936163186	38.0	37.0	38.0	35.0	38.0
56-57	36.605654841905576	38.0	37.0	38.0	34.0	38.0
58-59	36.654611503221794	38.0	37.0	38.0	34.0	38.0
60-61	36.2706382913361	38.0	37.0	38.0	34.0	38.0
62-63	36.17487155068468	38.0	37.0	38.0	34.0	38.0
64-65	36.63081477954334	38.0	37.0	38.0	34.0	38.0
66-67	36.290909858916955	38.0	37.0	38.0	33.0	38.0
68-69	36.25904521262298	38.0	37.0	38.0	34.0	38.0
70-71	34.29322924266745	38.0	34.0	38.0	26.0	38.0
72-73	30.844132803919912	37.0	23.0	38.0	11.0	38.0
74-75	30.30594745290192	37.0	23.0	38.0	11.0	38.0
76	30.21287641985454	37.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	1.0
23	17.0
24	27.0
25	51.0
26	138.0
27	234.0
28	535.0
29	868.0
30	1437.0
31	2303.0
32	3620.0
33	5820.0
34	10130.0
35	22682.0
36	25721.0
37	56621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.414427428711935	15.002189932457874	11.981619935300943	46.601762703529246
2	13.609867365041817	14.314130576697107	52.248343023032554	19.82765903522852
3	12.653697573863157	16.840108442710452	50.889737110908015	19.616456872518377
4	8.449622524134647	39.18376124171512	32.716367015598244	19.650249218552
5	7.84596834271583	29.017641140645278	54.018601150475774	9.117789366163109
6	18.081977159446115	55.5039283602264	16.08208468054713	10.33200979978035
7	17.056686660471403	24.719869131459905	18.674879230763324	39.54856497730537
8	14.818711743608254	53.92183215956131	14.29032233290069	16.96913376392974
9	15.678880551736848	15.512222845161935	15.203483683672921	53.6054129194283
10-11	16.093604798513137	33.9586197362661	32.013255815739555	17.934519649481214
12-13	15.933859162717825	17.085102951454225	34.9647100386308	32.016327847197154
14-15	15.292188592011183	16.602794012610687	19.345350096384987	48.75966729899314
16-17	30.56709700707335	31.905350710791282	16.757163593355195	20.770388688780173
18-19	31.51405070387921	21.586397044705738	17.691061156466244	29.208491094948812
20-21	15.815969955532344	18.40722849001974	17.331633475926793	48.44516807852112
22-23	15.416605866044067	33.93212346494428	17.430322486502263	33.22094818250939
24-25	30.54786472153634	33.66178848051734	17.609855190448943	18.18049160749738
26-27	31.13043077561114	35.53610788974479	17.968311995514835	15.365149339129234
28-29	16.050212354174505	35.386346356186685	18.493245370832597	30.07019591880621
30-31	14.99727357208138	35.05264693910466	34.079580974909184	15.870498513904781
32-33	28.102943774144247	37.19730890044314	19.88641163685516	14.81333568855745
34-35	18.048952821276888	19.99969279685424	47.537382782799696	14.413971599069175
36-37	14.861336180082485	46.80470328016159	23.001067530931515	15.33289300882441
38-39	14.737302909981798	22.90045850069505	33.59035996528604	28.77187862403711
40-41	14.49384441696683	17.275952905757755	35.855215157403215	32.37498751987221
42-43	29.618337026185326	17.400052992746136	34.51133391958159	18.470276061486945
44-45	15.385885551468048	32.24404217899191	36.335604076585746	16.034468192954296
46-47	29.349420538066312	17.87538304392237	23.00337155452472	29.7718248634866
48-49	28.52496409563234	16.40234396000215	20.28692773814004	34.78576420622547
50-51	16.351667428400244	16.505678435770495	35.6821612237922	31.460492912037054
52-53	12.68202368468562	16.483250556010095	50.964327006917955	19.870398752386333
54-55	12.262697439956353	17.048899407920267	38.75298249119181	31.935420660931573
56-57	27.21100720032582	16.82778388264314	24.151829282349595	31.809379634681438
58-59	13.942446208986873	17.564239972648313	48.220795734365936	20.27251808399888
60-61	27.57745126284452	31.927782579467973	23.354652837798906	17.1401133198886
62-63	13.07176989382788	50.57618733002474	18.57128589647066	17.78075687967672
64-65	11.74710710157117	52.56337876710487	17.541353181324144	18.14816094999981
66-67	12.118290185894457	52.16146963800714	17.004022142914764	18.71621803318364
68-69	12.641276839239149	52.29019213266102	16.67441137050535	18.394119657594484
70-71	13.136367825980091	49.49113616812093	16.950734300110607	20.421761705788374
72-73	13.718870504370804	43.40961115976095	17.510869398227097	25.36064893764115
74-75	14.541376714748761	40.94274697758729	18.4371015561393	26.078774751524648
76	14.656589397360861	41.494231685023884	18.881822511022015	24.96735640659324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.0
14	4.0
15	4.5
16	1.0
17	1.0
18	6.5
19	15.0
20	26.0
21	34.0
22	51.5
23	85.5
24	141.0
25	180.0
26	214.0
27	269.0
28	373.0
29	456.0
30	500.5
31	625.5
32	881.5
33	1057.0
34	1320.0
35	2042.5
36	7691.5
37	12881.0
38	15341.5
39	16457.0
40	13021.0
41	9531.0
42	8132.0
43	7583.5
44	7096.0
45	6902.0
46	6647.0
47	6615.5
48	6369.0
49	5765.0
50	5376.0
51	4920.0
52	3905.0
53	3118.5
54	2891.0
55	2507.0
56	1893.5
57	1328.5
58	993.0
59	838.5
60	592.5
61	448.5
62	396.0
63	336.0
64	271.0
65	230.5
66	174.0
67	153.0
68	142.5
69	136.5
70	121.0
71	101.0
72	83.0
73	62.0
74	54.0
75	49.0
76	40.0
77	28.0
78	16.0
79	7.0
80	5.5
81	4.5
82	4.5
83	4.0
84	2.5
85	1.0
86	1.0
87	1.0
88	1.5
89	1.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05068851905043508
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0011520117966007971
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.840039322002657E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.016512169084611426
52-53	0.029568302779420463
54-55	0.05568057016903853
56-57	0.056832581965639326
58-59	0.038784397152226834
60-61	0.03417634996582365
62-63	0.03148832244042179
64-65	0.0134401376270093
66-67	0.0403204128810279
68-69	0.00806408257620558
70-71	0.011520117966007973
72-73	0.019200196610013286
74-75	0.009984102237206909
76	0.009984102237206909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	130207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.25565445790165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.953888311442874	5.844539848088044
2	10.932627799300764	3.55434039644566
3	11.324766134366438	5.522744552904222
4	10.436549182651422	6.786117489843097
5	8.003401681942739	6.505026611472502
6	6.014362657091562	5.866044068291259
7	3.926107908910517	4.4675017472178915
8	2.6552017386374374	3.452963358344789
9	2.1638476802418976	3.1657284170589906
>10	8.400264575262213	21.57487692673973
>50	0.09449116507606539	0.9500257282634573
>100	0.07086837380704905	2.2402789404563506
>500	0.009449116507606539	1.0775150337539456
>1k	0.009449116507606539	4.799281144638921
>5k	0.0	0.0
>10k+	0.004724558253803269	24.19301573648114
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	31501	24.19301573648114	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	3857	2.96220633299285	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	2392	1.8370748116460711	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	723	0.5552696859615842	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	680	0.5222453477923614	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	396	0.3041311143026105	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT	363	0.27878685477739296	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	299	0.2296343514557589	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT	244	0.18739391891372967	No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	229	0.1758738009477217	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	212	0.16281766725291266	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC	195	0.14976153355810365	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGT	150	0.11520117966007971	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT	145	0.11136114033807706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.680078644005314E-4	0.0
2	0.0	0.0	0.0	0.0023040235932015943	0.0
3	0.0	0.0	0.0	0.0023040235932015943	0.0
4	0.0	0.0	0.0	0.006144062915204251	0.0
5	0.0	0.0	0.0	0.012288125830408503	0.0
6	0.0	0.0	0.0	0.012288125830408503	0.0
7	0.0	0.0	0.0	0.012288125830408503	0.0
8	0.0	0.0	0.0	0.012288125830408503	0.0
9	0.0	0.0	0.0	0.012288125830408503	0.0
10	0.0	0.0	0.0	0.013824141559209566	0.0
11	0.0	0.0	0.0	0.01612816515241116	0.0
12	0.0	0.0	0.0	0.01612816515241116	0.0
13	0.0	0.0	0.0	0.018432188745612754	0.0
14	0.0	0.0	0.0	0.018432188745612754	0.0
15	0.0	0.0	0.0	0.02227222806761541	0.0
16	0.0	0.0	0.0	0.02227222806761541	0.0
17	0.0	0.0	0.0	0.02227222806761541	0.0
18	0.0	0.0	0.0	0.02227222806761541	0.0
19	0.0	0.0	0.0	0.025344259525217537	0.0
20	0.0	0.0	0.0	0.03609636962682498	0.0
21	0.0	0.0	0.0	0.03763238535562604	0.0
22	0.0	0.0	0.0	0.04300844040642976	0.0
23	0.0	0.0	0.0	0.045312463999631354	0.0
24	0.0	0.0	0.0	0.04761648759283295	0.0
25	0.0	0.0	0.0	0.051456526914835606	0.0
26	0.0	0.0	0.0	0.05299254264363667	0.0
27	0.0	0.0	0.0	0.06681668420284624	0.0
28	0.0	0.0	0.0	0.0829448493552574	0.0
29	0.0	0.0	0.0	0.12902532121928928	0.0
30	0.0	0.0	0.0	0.15283356501570577	0.0
31	0.0	0.0	0.0	0.17740981667652278	0.0
32	0.0	0.0	0.0	0.21811423348975092	0.0
33	0.0	0.0	0.0	0.2649627132181834	0.0
34	0.0	0.0	0.0	0.3156512322686184	0.0
35	0.0	0.0	0.0	0.42317233328469284	0.0
36	0.0	0.0	0.0	0.6197823465712289	0.0
37	0.0	0.0	0.0	1.0091623338222984	0.0
38	0.0	0.0	0.0	1.4707350603270177	0.0
39	0.0	0.0	0.0	1.9691721643229627	0.0
40	0.0	0.0	0.0	2.570522322148579	0.0
41	0.0	0.0	0.0	3.2663374472954603	0.0
42	0.0	0.0	0.0	3.8738316680362805	0.0
43	0.0	0.0	0.0	4.571950816776364	0.0
44	0.0	0.0	0.0	5.25931785541484	0.0
45	0.0	0.0	0.0	6.163263111814265	0.0
46	0.0	0.0	0.0	7.153993256890951	0.0
47	0.0	0.0	0.0	8.226132235594093	0.0
48	0.0	0.0	0.0	9.196894176196365	0.0
49	0.0	0.0	0.0	10.110823534832997	0.0
50	0.0	0.0	0.0	10.944112067707573	0.0
51	0.0	0.0	0.0	11.751288333192532	0.0
52	0.0	0.0	0.0	12.525440260508267	0.0
53	0.0	0.0	0.0	13.2934481249088	0.0
54	0.0	0.0	0.0	13.950862856835654	0.0
55	0.0	0.0	0.0	14.603669541576105	0.0
56	0.0	0.0	0.0	15.258780249909758	0.0
57	0.0	0.0	0.0	15.865506462786179	0.0
58	0.0	0.0	0.0	16.52829724976384	0.0
59	0.0	0.0	0.0	17.18263995023309	0.0
60	0.0	0.0	0.0	17.94987980676922	0.0
61	0.0	0.0	0.0	18.577342231984456	0.0
62	0.0	0.0	0.0	19.10265961123442	0.0
63	0.0	0.0	0.0	19.662537344382407	0.0
64	0.0	0.0	0.0	20.161742456242752	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGT	15	0.0022205126	69.99962	34
GAATGCA	15	0.0022205126	69.99962	53
TCTTCCG	15	0.0022205126	69.99962	50
CGTGATA	15	0.0022205126	69.99962	24
CCGGATC	15	0.0022205126	69.99962	33
TTCGTGA	15	0.0022205126	69.99962	22
CCGCTTG	15	0.0022205126	69.99962	54
CGGTGGA	15	0.0022205126	69.99962	37
TGCTCGA	15	0.0022205126	69.99962	55
CTCGAAA	15	0.0022205126	69.99962	57
GTCTTCC	15	0.0022205126	69.99962	49
GGTTCAC	15	0.0022205126	69.99962	33
GTGGTTC	15	0.0022205126	69.99962	31
TCGGTGG	15	0.0022205126	69.99962	36
CTGGATA	15	0.0022205126	69.99962	39
CGCTTGA	15	0.0022205126	69.99962	55
TCTGCCG	20	7.902499E-5	69.99961	43
CGTGCGA	20	7.902499E-5	69.99961	10
CCGTGCG	20	7.902499E-5	69.99961	9
AGTCTTC	20	7.902499E-5	69.99961	48
>>END_MODULE