FastQCFastQC Report
Wed 25 May 2016
SRR1779856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32234
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT150.002205263870.020
TGTGGTC150.002205263870.047
ACATTTG150.002205263870.08
GGATAGT207.812356E-570.031
GTGTGTA401.2914825E-1070.034
TATCTCG150.002205263870.041
TTGACGC150.002205263870.012
CAGTGTG207.812356E-570.032
CGTGTAG150.002205263870.035
TTACGTG150.002205263870.032
TAGATCC207.812356E-570.039
ATAGTGT309.930045E-870.033
AATAGCA150.002205263870.01
TGCAGTG207.812356E-570.030
TGCAGTC150.002205263870.047
ATGCAGT207.812356E-570.029
GAGGGTA150.002205263870.026
AGTGTGT309.930045E-870.033
GATAGTG207.812356E-570.032
CTGTGGT252.7787446E-670.046