FastQCFastQC Report
Wed 25 May 2016
SRR1779856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32234
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1939960.181795619532174No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG25287.842650617360551No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC11753.645219333622882No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT5481.7000682509151828Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5181.606998821120556No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3631.126140100514984No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT3431.064093813985233No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2260.7011230377861886No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1520.47155177762610906No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT1500.465347148973134No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1410.4374263200347459No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT1360.42191474840230814No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG930.28851523236334303No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC880.27300366073090526No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT870.2699013464044177No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG820.2543897747719799No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC750.232673574486567No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG720.2233666315071043No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT550.17062728795681578No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTACGATCTCGTATGCCGT500.15511571632437798No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTCAGATCTCGTATGCCGT500.15511571632437798No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCG490.15201340199789043No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTAAGATCTCGTATGCCGT490.15201340199789043No Hit
GCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG470.1458087733449153No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT460.14270645901842777No Hit
CTTATACACATCTCCGAGCCCACGAGAATTGCTAAGATCTCGTATGCCGT450.13960414469194019No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCG450.13960414469194019No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCC450.13960414469194019No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTCTGCCGT420.1302972017124775No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT340.10547868710057703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCA150.002205263870.042
CAGTCTT150.002205263870.047
CTTTACA150.002205263870.01
AAACAGT150.002205263870.068
TATGCAG150.002205263870.043
GAAAACA150.002205263870.060
TTACACA150.002205263870.03
GCAGTCT150.002205263870.046
ATGCAGT207.812356E-570.044
AGTCTTC150.002205263870.048
TGCAGTC150.002205263870.045
TTTACAC150.002205263870.02
TGAAAAC150.002205263870.059
ACAACCC207.812356E-570.070
ACAACAA150.002205263870.070
GCTTATA207.812356E-570.01
CAAAAAC150.002205263870.066
TCTTTAT501.2732926E-1162.9999961
TGACAAA352.885463E-760.059
GCTTGAC352.885463E-760.056
Produced by FastQC (version 0.11.5)