##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779856_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32234
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.48786995098343	34.0	34.0	34.0	34.0	34.0
2	33.59955326673698	34.0	34.0	34.0	34.0	34.0
3	33.74154619346032	34.0	34.0	34.0	34.0	34.0
4	33.542905007135325	34.0	34.0	34.0	34.0	34.0
5	33.598281317863126	34.0	34.0	34.0	34.0	34.0
6	37.38679655022647	38.0	38.0	38.0	37.0	38.0
7	37.38115033815226	38.0	38.0	38.0	37.0	38.0
8	37.32316808339021	38.0	38.0	38.0	37.0	38.0
9	37.223925048085874	38.0	38.0	38.0	36.0	38.0
10-11	37.1651206800273	38.0	38.0	38.0	36.5	38.0
12-13	37.3244555438357	38.0	38.0	38.0	36.5	38.0
14-15	37.34285226779177	38.0	38.0	38.0	37.0	38.0
16-17	37.033753179872186	38.0	38.0	38.0	35.0	38.0
18-19	36.793385865855925	38.0	37.5	38.0	34.0	38.0
20-21	36.98455047465409	38.0	38.0	38.0	35.0	38.0
22-23	37.06030899050692	38.0	38.0	38.0	35.5	38.0
24-25	37.06860768133028	38.0	38.0	38.0	35.5	38.0
26-27	36.93590618601476	38.0	38.0	38.0	35.0	38.0
28-29	36.407613079357205	38.0	37.0	38.0	34.0	38.0
30-31	36.45514053483899	38.0	37.0	38.0	34.0	38.0
32-33	36.41685797605013	38.0	37.0	38.0	34.0	38.0
34-35	36.13128994229696	38.0	37.0	38.0	34.0	38.0
36-37	36.24066203387727	38.0	37.0	38.0	34.0	38.0
38-39	32.3054228454427	36.0	30.5	37.0	17.5	38.0
40-41	35.342340385927905	37.5	35.5	38.0	30.5	38.0
42-43	36.91890550350561	38.0	37.0	38.0	35.0	38.0
44-45	36.71424582738723	38.0	37.0	38.0	35.0	38.0
46-47	36.91198734255755	38.0	37.5	38.0	35.5	38.0
48-49	36.32369547682571	38.0	37.0	38.0	34.0	38.0
50-51	36.32090339393187	38.0	37.0	38.0	33.5	38.0
52-53	36.24163926289012	38.0	37.0	38.0	34.0	38.0
54-55	36.31685487373581	38.0	37.0	38.0	34.0	38.0
56-57	36.132468821741014	38.0	37.0	38.0	33.0	38.0
58-59	36.056694794316556	38.0	37.0	38.0	33.0	38.0
60-61	35.458304895452	38.0	36.5	38.0	29.0	38.0
62-63	35.52351554259478	38.0	36.5	38.0	31.0	38.0
64-65	36.20641248371285	38.0	37.0	38.0	34.0	38.0
66-67	35.58914500217162	38.0	36.5	38.0	31.0	38.0
68-69	35.18280387168828	38.0	36.0	38.0	29.0	38.0
70-71	30.132577402742445	33.5	27.5	37.0	16.5	38.0
72-73	21.303685549419868	22.0	11.0	28.0	10.0	37.0
74-75	20.029844263820813	21.0	11.0	25.0	10.5	35.5
76	20.30936278463734	21.0	11.0	25.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	5.0
24	14.0
25	32.0
26	55.0
27	132.0
28	239.0
29	436.0
30	683.0
31	1054.0
32	1551.0
33	2558.0
34	4688.0
35	10831.0
36	7998.0
37	1958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	4.308418177303204	3.85212316861187	13.539855972187734	78.2996026818972
2	1.994788111931501	2.47874914686356	80.93007383508098	14.59638890612397
3	2.1902339145002174	5.131227896010423	85.409815722529	7.268722466960352
4	1.5232363343053916	70.99956567599429	21.830985915492956	5.646212074207359
5	1.4953155053670037	17.590122231184463	78.7274306632748	2.1871316001737298
6	3.1426444127318978	78.93838803747596	12.843581311658497	5.075386238133648
7	2.6866042067382265	17.348141713718434	8.78575417261277	71.17949990693056
8	2.2088478004591425	79.18036855494198	4.324626171123659	14.28615747347521
9	2.2026431718061676	14.388533846249302	2.4973630328224856	80.91145994912205
10-11	2.522181547434386	45.541974312837375	38.80529875286964	13.130545386858596
12-13	3.397034187503878	5.747037289818204	46.415275795743625	44.440652726934296
14-15	4.222249798349568	3.3272321151579076	11.118694546131414	81.33182354036111
16-17	42.89104672085376	37.65278898057951	2.480300304026804	16.975863994539928
18-19	45.42563752559409	12.182788360116648	2.6509275919836197	39.74064652230564
20-21	6.739777874294224	3.601786933052057	2.5392442762300673	87.11919091642365
22-23	4.281193770552832	43.30055221195012	2.3360426878451324	50.08221132965191
24-25	43.00428119377055	42.1945771545573	2.4554817894149035	12.345659862257245
26-27	45.59161134206118	46.38425265247875	4.577464788732395	3.4466712167276787
28-29	6.9166097909040145	11.63833219581808	38.26394490289756	43.181113110380345
30-31	37.2308742321772	3.63591239064342	52.19643854315319	6.936774834026184
32-33	12.199851088912329	4.897003164360613	40.990879195880126	41.91226655084693
34-35	3.629707761990445	77.73003660730905	12.40150152013402	6.238754110566483
36-37	41.81454364956257	49.86660048396103	4.4518210585096485	3.867034807966743
38-39	7.079481293044611	12.553514922131912	41.14289259787802	39.22411118694546
40-41	3.2093441707513803	3.8205000930694295	46.63709127008748	46.333064466091706
42-43	42.900353663833215	3.13954209840541	42.562201402246075	11.397902835515294
44-45	6.614134144071477	41.74629273437985	46.7580815288205	4.8814915927281755
46-47	42.88949556369051	5.722218775206303	12.691567909660606	38.69671775144258
48-49	40.5968852764162	2.4430725321089533	4.95749829372712	52.00254389774772
50-51	10.727250162886662	2.430889516304179	44.575098507647915	42.26676181316124
52-53	2.918541505042669	2.4313421256788206	82.30721489526765	12.342901474010862
54-55	2.7887271485769265	2.514044507899066	51.3268568236134	43.37037151991061
56-57	42.526149166640806	2.5590490083491106	13.675160619510226	41.23964120549986
58-59	6.319333467798241	4.738336462228291	77.33542271112283	11.606907358850634
60-61	41.5974926688492	40.36554902173744	14.570759181393617	3.466199128019736
62-63	5.180679896355371	88.41135125909607	2.8052318815844597	3.602736962964112
64-65	1.9267762953769778	92.1827489916227	2.4713000310269932	3.4191746819733164
66-67	2.0729569116665894	92.0184952443017	2.293286164253906	3.6152616797778085
68-69	2.041417823625223	91.6326688900954	2.305126812999302	4.020786473280075
70-71	2.87615767673477	84.78614976497417	3.7619645987496315	8.575727959541428
72-73	5.769827479210624	67.10469157254562	6.995469777832939	20.13001117041082
74-75	7.744097297632714	59.42415686761192	9.40088734448202	23.43085849027334
76	7.207794222594558	62.335163982748455	8.18207204691427	22.274969747742716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	4.0
20	4.0
21	1.0
22	5.0
23	11.0
24	9.0
25	5.0
26	11.5
27	14.0
28	20.5
29	31.0
30	43.5
31	51.0
32	44.0
33	42.0
34	47.5
35	57.5
36	91.0
37	120.0
38	233.5
39	3254.5
40	7649.5
41	8234.0
42	7331.0
43	5667.0
44	2962.0
45	1367.0
46	813.0
47	596.5
48	317.0
49	194.0
50	134.0
51	130.5
52	128.0
53	128.0
54	127.0
55	130.5
56	115.5
57	92.5
58	88.0
59	90.5
60	80.5
61	74.5
62	81.0
63	71.0
64	55.0
65	48.0
66	40.5
67	34.0
68	24.0
69	15.0
70	17.0
71	18.0
72	17.5
73	14.5
74	14.0
75	16.0
76	14.0
77	11.0
78	7.0
79	4.0
80	6.0
81	6.5
82	4.0
83	3.0
84	2.5
85	2.5
86	1.5
87	0.0
88	1.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05584165787677608
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.00930694297946268
52-53	0.02792082893838804
54-55	0.046534714897313395
56-57	0.046534714897313395
58-59	0.023267357448656698
60-61	0.02636967177514426
62-63	0.02636967177514426
64-65	0.01240925730595024
66-67	0.029471986101631816
68-69	0.00465347148973134
70-71	0.010858100142706458
72-73	0.01861388595892536
74-75	0.00930694297946268
76	0.015511571632437798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	32234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.632685983743873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42995169082126	7.073276664391637
2	10.72463768115942	2.066141341440715
3	5.603864734299517	1.6194080784265061
4	3.2850241545893724	1.2657442452069243
5	1.964573268921095	0.9462058695787057
6	1.2560386473429952	0.725941552398089
7	0.644122383252818	0.43432400570825835
8	0.3864734299516908	0.29782217534280575
9	0.322061191626409	0.27920828938388037
>10	1.7713365539452495	3.9151206800273
>50	0.22544283413848631	1.712477508221133
>100	0.22544283413848631	4.687596947322703
>500	0.06441223832528181	3.3070670720357387
>1k	0.06441223832528181	11.487869950983434
>5k	0.0	0.0
>10k+	0.032206119162640906	60.181795619532174
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	19399	60.181795619532174	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	2528	7.842650617360551	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1175	3.645219333622882	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	548	1.7000682509151828	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	518	1.606998821120556	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	363	1.126140100514984	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT	343	1.064093813985233	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	226	0.7011230377861886	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	152	0.47155177762610906	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT	150	0.465347148973134	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	141	0.4374263200347459	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT	136	0.42191474840230814	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG	93	0.28851523236334303	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	88	0.27300366073090526	No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT	87	0.2699013464044177	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	82	0.2543897747719799	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	75	0.232673574486567	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	72	0.2233666315071043	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT	55	0.17062728795681578	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTACGATCTCGTATGCCGT	50	0.15511571632437798	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTCAGATCTCGTATGCCGT	50	0.15511571632437798	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCG	49	0.15201340199789043	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTAAGATCTCGTATGCCGT	49	0.15201340199789043	No Hit
GCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	47	0.1458087733449153	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT	46	0.14270645901842777	No Hit
CTTATACACATCTCCGAGCCCACGAGAATTGCTAAGATCTCGTATGCCGT	45	0.13960414469194019	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCG	45	0.13960414469194019	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCC	45	0.13960414469194019	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTCTGCCGT	42	0.1302972017124775	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT	34	0.10547868710057703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.012409257305950239	0.0
6	0.0	0.0	0.0	0.01861388595892536	0.0
7	0.0	0.0	0.0	0.01861388595892536	0.0
8	0.0	0.0	0.0	0.01861388595892536	0.0
9	0.0	0.0	0.0	0.021716200285412917	0.0
10	0.0	0.0	0.0	0.021716200285412917	0.0
11	0.0	0.0	0.0	0.046534714897313395	0.0
12	0.0	0.0	0.0	0.046534714897313395	0.0
13	0.0	0.0	0.0	0.046534714897313395	0.0
14	0.0	0.0	0.0	0.046534714897313395	0.0
15	0.0	0.0	0.0	0.06825091518272632	0.0
16	0.0	0.0	0.0	0.06825091518272632	0.0
17	0.0	0.0	0.0	0.06825091518272632	0.0
18	0.0	0.0	0.0	0.07135322950921387	0.0
19	0.0	0.0	0.0	0.07755785816218899	0.0
20	0.0	0.0	0.0	0.08376248681516411	0.0
21	0.0	0.0	0.0	0.08376248681516411	0.0
22	0.0	0.0	0.0	0.08376248681516411	0.0
23	0.0	0.0	0.0	0.08686480114165167	0.0
24	0.0	0.0	0.0	0.08686480114165167	0.0
25	0.0	0.0	0.0	0.08686480114165167	0.0
26	0.0	0.0	0.0	0.08686480114165167	0.0
27	0.0	0.0	0.0	0.08686480114165167	0.0
28	0.0	0.0	0.0	0.10237637277408947	0.0
29	0.0	0.0	0.0	0.11168331575355216	0.0
30	0.0	0.0	0.0	0.12719488738598994	0.0
31	0.0	0.0	0.0	0.1458087733449153	0.0
32	0.0	0.0	0.0	0.15511571632437798	0.0
33	0.0	0.0	0.0	0.17062728795681578	0.0
34	0.0	0.0	0.0	0.18613885958925358	0.0
35	0.0	0.0	0.0	0.22646894583359187	0.0
36	0.0	0.0	0.0	0.3071291183222684	0.0
37	0.0	0.0	0.0	0.4374263200347459	0.0
38	0.0	0.0	0.0	0.49637029223800955	0.0
39	0.0	0.0	0.0	0.6452813799094124	0.0
40	0.0	0.0	0.0	0.7600670099894521	0.0
41	0.0	0.0	0.0	0.8841595830489545	0.0
42	0.0	0.0	0.0	1.0020475274554819	0.0
43	0.0	0.0	0.0	1.1385493578209345	0.0
44	0.0	0.0	0.0	1.2657442452069243	0.0
45	0.0	0.0	0.0	1.408450704225352	0.0
46	0.0	0.0	0.0	1.5449525345908048	0.0
47	0.0	0.0	0.0	1.7466029658124962	0.0
48	0.0	0.0	0.0	1.8520816529130733	0.0
49	0.0	0.0	0.0	1.994788111931501	0.0
50	0.0	0.0	0.0	2.08475522739964	0.0
51	0.0	0.0	0.0	2.1747223428677795	0.0
52	0.0	0.0	0.0	2.246075572376993	0.0
53	0.0	0.0	0.0	2.401191288701371	0.0
54	0.0	0.0	0.0	2.488056089843023	0.0
55	0.0	0.0	0.0	2.587330148290625	0.0
56	0.0	0.0	0.0	2.658683377799839	0.0
57	0.0	0.0	0.0	2.767264379226903	0.0
58	0.0	0.0	0.0	2.87274306632748	0.0
59	0.0	0.0	0.0	2.9471986101631815	0.0
60	0.0	0.0	0.0	3.043370354284296	0.0
61	0.0	0.0	0.0	3.11472358379351	0.0
62	0.0	0.0	0.0	3.2015883849351616	0.0
63	0.0	0.0	0.0	3.226406899547062	0.0
64	0.0	0.0	0.0	3.2946578147297885	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCA	15	0.0022052638	70.0	42
CAGTCTT	15	0.0022052638	70.0	47
CTTTACA	15	0.0022052638	70.0	1
AAACAGT	15	0.0022052638	70.0	68
TATGCAG	15	0.0022052638	70.0	43
GAAAACA	15	0.0022052638	70.0	60
TTACACA	15	0.0022052638	70.0	3
GCAGTCT	15	0.0022052638	70.0	46
ATGCAGT	20	7.812356E-5	70.0	44
AGTCTTC	15	0.0022052638	70.0	48
TGCAGTC	15	0.0022052638	70.0	45
TTTACAC	15	0.0022052638	70.0	2
TGAAAAC	15	0.0022052638	70.0	59
ACAACCC	20	7.812356E-5	70.0	70
ACAACAA	15	0.0022052638	70.0	70
GCTTATA	20	7.812356E-5	70.0	1
CAAAAAC	15	0.0022052638	70.0	66
TCTTTAT	50	1.2732926E-11	62.999996	1
TGACAAA	35	2.885463E-7	60.0	59
GCTTGAC	35	2.885463E-7	60.0	56
>>END_MODULE