FastQCFastQC Report
Wed 25 May 2016
SRR1779855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86398
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT207.887228E-570.0000142
AGATCTA207.887228E-570.0000140
GTCGCGT150.002217923570.08
CTTCCGA252.8187897E-670.018
TTCCGAC252.8187897E-670.019
AGTCGCG150.002217923570.07
GACGAAT453.783498E-1062.22221823
GCCGTAT352.943616E-760.054
TAGATCT950.055.2631639
ACGAATA452.6646376E-854.44444324
GATCTCG850.053.52940841
CTGCTGG200.00693039152.50000418
CGTATCA407.403287E-752.50000456
GGTCGAT200.00693039152.5000047
GAGTCGC200.00693039152.5000046
CGTTGTT200.00693039152.50000455
CGGCACA200.00693039152.5000042
TCTTACG200.00693039152.50000411
ATGTCGG200.00693039152.5000047
TACGCTG200.00693039152.50000414