##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779855_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86398
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.860957429570128	32.0	21.0	34.0	12.0	34.0
2	27.096495289242807	32.0	21.0	34.0	12.0	34.0
3	26.840204634366536	33.0	21.0	34.0	11.0	34.0
4	26.941468552512788	33.0	21.0	34.0	12.0	34.0
5	27.024028333989214	34.0	21.0	34.0	11.0	34.0
6	29.869198361073174	36.0	23.0	38.0	11.0	38.0
7	30.007801106507095	36.0	23.0	38.0	12.0	38.0
8	29.49268501585685	36.0	21.0	38.0	11.0	38.0
9	30.344718627746012	37.0	24.0	38.0	11.0	38.0
10-11	29.005648278895343	34.5	21.0	38.0	11.0	38.0
12-13	29.65698280052779	36.0	22.0	38.0	11.0	38.0
14-15	28.603225769115028	34.0	21.0	38.0	11.0	38.0
16-17	29.051436375842034	35.0	21.5	38.0	11.0	38.0
18-19	29.567368457603187	36.0	21.0	38.0	11.0	38.0
20-21	28.737204564920482	34.5	22.0	38.0	11.0	38.0
22-23	29.386791360911133	35.5	22.0	38.0	11.0	38.0
24-25	29.629997222157918	36.5	22.0	38.0	11.0	38.0
26-27	28.709264103335727	34.0	21.0	38.0	11.0	38.0
28-29	29.55577096692053	36.5	21.5	38.0	11.0	38.0
30-31	29.87224820018982	37.0	22.0	38.0	11.0	38.0
32-33	30.009751383133867	37.0	22.5	38.0	11.0	38.0
34-35	30.02801569480775	37.0	23.0	38.0	11.0	38.0
36-37	29.621073404476956	36.5	21.5	38.0	11.0	38.0
38-39	29.52533044746406	36.5	21.0	38.0	11.0	38.0
40-41	29.438088844649183	36.0	21.0	38.0	11.0	38.0
42-43	29.80715988796037	36.5	22.0	38.0	11.0	38.0
44-45	29.887289057617075	37.0	23.0	38.0	11.0	38.0
46-47	29.75	37.0	22.0	38.0	11.0	38.0
48-49	29.494455890182643	36.5	21.5	38.0	11.0	38.0
50-51	28.96363920461122	35.0	21.0	38.0	11.0	38.0
52-53	29.469993518368483	36.0	21.5	38.0	11.0	38.0
54-55	29.595899210629874	36.5	21.5	38.0	11.0	38.0
56-57	29.5422347739531	36.5	21.5	38.0	11.0	38.0
58-59	29.763675085071412	37.0	22.0	38.0	11.0	38.0
60-61	29.722863955184145	37.0	22.0	38.0	11.0	38.0
62-63	29.64846987198778	37.0	21.5	38.0	11.0	38.0
64-65	29.657874024861684	36.5	21.5	38.0	11.0	38.0
66-67	29.706983957961988	36.0	22.5	38.0	11.0	38.0
68-69	29.181069006227	35.5	21.5	38.0	11.0	38.0
70-71	29.240694229033082	35.5	21.5	38.0	11.0	38.0
72-73	29.421734299405077	36.0	22.5	38.0	11.0	38.0
74-75	29.569718049028914	36.0	22.5	38.0	11.0	38.0
76	28.535892034537838	34.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	27.0
13	342.0
14	1380.0
15	2818.0
16	3768.0
17	4563.0
18	4492.0
19	3797.0
20	3050.0
21	2102.0
22	1525.0
23	1236.0
24	1040.0
25	959.0
26	1000.0
27	1055.0
28	1232.0
29	1341.0
30	1670.0
31	1967.0
32	2478.0
33	3202.0
34	4328.0
35	6438.0
36	10957.0
37	19631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.736845760318527	20.18218014305887	9.731706752471123	38.349267344151485
2	15.957545313548923	20.131253038264774	31.343318132364175	32.567883515822125
3	19.867358040695386	20.62432000740758	31.680131484525102	27.828190467371925
4	17.266603393597073	24.682284312136854	27.541146785805225	30.509965508460844
5	12.181994953586889	30.67779346744138	30.878029584018147	26.262181994953586
6	23.128653278546214	42.20846113779733	14.724231726373056	19.93865385728341
7	21.977220640322244	33.63543764613283	17.659791189202952	26.72755052434197
8	19.69882864947451	39.62567711468124	18.231168109634705	22.444326126209546
9	22.408065468267104	18.728369196578427	18.001458451494912	40.86210688365956
10-11	20.433119397197803	28.990757727454238	23.46275601442188	27.11336686092608
12-13	19.90439593509109	23.377277251788236	25.857658742100508	30.860668071020164
14-15	25.287622398666638	25.901062524595474	21.107548785851524	27.70376629088636
16-17	26.854943719436324	25.57655024740299	20.838565930727164	26.72994010243352
18-19	21.99774299024856	24.851992245145983	23.97002228073729	29.18024248386817
20-21	24.506933030857194	24.24419546748767	23.04335748512697	28.20551401652816
22-23	26.985624334459928	24.31594055280337	21.59648594842354	27.10194916431316
24-25	21.530840191210345	29.90150120953274	21.051656886234476	27.51600171302244
26-27	23.431812263086314	29.89611967938887	22.633756763795247	24.038311293729564
28-29	21.819602396041322	28.212518447781925	25.069305784645657	24.898573371531093
30-31	22.79856015185537	26.60362508391398	25.171300261580132	25.426514502650527
32-33	19.923493599388877	29.208430750711823	24.710062732933633	26.158012916965674
34-35	19.64592653467524	31.188327960691943	24.2202918009827	24.945453703650116
36-37	23.896863062854496	25.735038777636298	24.019562449357565	26.348535710151637
38-39	24.103086459050736	24.421976190338395	26.51877745432237	24.956159896288494
40-41	21.280129633380596	27.81561966492086	24.93069822622182	25.97355247547672
42-43	18.53294366156428	26.9232327324286	26.34277612199427	28.201047484012847
44-45	18.159615718502227	24.363099716418773	29.034087620811388	28.44319694426761
46-47	19.643270521783414	26.76512801518589	26.8397842492708	26.7518172137599
48-49	20.343759946757718	27.353222026100294	25.740906854943717	26.56211117219827
50-51	23.950158575827025	26.55334861217214	23.227886195800636	26.268606616200195
52-53	16.97726641355681	29.304217983146586	25.524354106861747	28.194161496434855
54-55	18.492453004907862	26.082276136679322	25.369825909806465	30.055444948606354
56-57	20.20615931334248	27.632987805230957	23.943303294960614	28.217549586465946
58-59	19.059855772013286	27.646977115671774	24.49387088932875	28.799296222986193
60-61	17.575662863989162	26.170177053127514	25.0791500986844	31.17500998419892
62-63	19.082317126106158	26.563955944739938	24.392446016101122	29.96128091305279
64-65	19.47861016733326	26.01566253205147	23.77336211936169	30.73236518125358
66-67	17.76625361014487	30.63775849794822	21.7829919491599	29.812995942747012
68-69	18.472756734859228	32.359153910703434	20.969935472669928	28.198153881767414
70-71	17.72147503414431	32.257691150258104	22.131878052732702	27.88895576286488
72-73	17.675382247068764	30.178131185110598	22.130976770026738	30.015509797793904
74-75	17.040055096737603	28.817561506363326	21.792148714891745	32.350234682007326
76	16.674190076044308	27.86439343958702	23.239232843733	32.22218364063567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	1.5
14	2.0
15	2.5
16	7.0
17	13.0
18	20.0
19	25.5
20	36.0
21	48.0
22	65.0
23	100.5
24	149.0
25	179.0
26	195.5
27	267.0
28	371.5
29	421.0
30	488.0
31	662.5
32	890.5
33	1011.0
34	1175.0
35	1511.0
36	1819.5
37	1956.0
38	2223.5
39	2839.5
40	3523.5
41	3972.5
42	4086.0
43	4346.5
44	4775.0
45	5118.0
46	5293.0
47	5277.5
48	5293.5
49	5134.0
50	4943.0
51	4781.5
52	4424.0
53	4047.5
54	3867.0
55	3660.5
56	3206.5
57	2739.5
58	2520.0
59	2305.5
60	1936.0
61	1638.5
62	1496.0
63	1343.5
64	1125.0
65	948.0
66	747.5
67	658.0
68	603.5
69	465.0
70	401.0
71	421.0
72	353.0
73	261.5
74	219.5
75	201.0
76	183.0
77	140.0
78	116.0
79	117.0
80	91.5
81	76.5
82	72.5
83	58.0
84	48.0
85	43.5
86	32.0
87	15.0
88	20.5
89	19.5
90	11.5
91	10.5
92	11.0
93	7.5
94	5.5
95	4.0
96	1.0
97	4.0
98	4.5
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003472302599597213
7	0.0046297367994629504
8	0.0023148683997314752
9	0.005787170999328688
10-11	0.0017361512997986065
12-13	0.0
14-15	0.0
16-17	5.787170999328688E-4
18-19	5.787170999328688E-4
20-21	0.0
22-23	0.0023148683997314752
24-25	0.0011574341998657376
26-27	5.787170999328688E-4
28-29	0.006365888099261557
30-31	0.0
32-33	0.0
34-35	0.004051019699530082
36-37	0.009259473598925901
38-39	0.005208453899395819
40-41	5.787170999328688E-4
42-43	5.787170999328688E-4
44-45	0.003472302599597213
46-47	0.0023148683997314752
48-49	5.787170999328688E-4
50-51	0.0046297367994629504
52-53	0.006944605199194426
54-55	0.006944605199194426
56-57	0.00983819069885877
58-59	0.008102039399060164
60-61	0.013310493298455982
62-63	0.007523322299127295
64-65	0.015625361698187457
66-67	0.010995624898724507
68-69	5.787170999328688E-4
70-71	0.0
72-73	0.0011574341998657376
74-75	0.005208453899395819
76	0.0011574341998657376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	86398.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.70198384221857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92156562953902	65.04432972985485
2	6.244170934943812	9.453922544503344
3	3.6510970109318857	8.291858607838144
4	1.8974084550110848	5.745503368133521
5	0.960171240730831	3.6343433875784164
6	0.5611191804907881	2.5486701081043543
7	0.28132405779374664	1.49077524942707
8	0.15748031496062992	0.9537257806893678
9	0.09326504089901383	0.6354313757262899
>10	0.23239813469918205	2.201439848144633
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.012731776198523115	0.0
6	0.0	0.0	0.0	0.012731776198523115	0.0
7	0.0	0.0	0.0	0.012731776198523115	0.0
8	0.0	0.0	0.0	0.012731776198523115	0.0
9	0.0	0.0	0.0	0.016204078798120327	0.0
10	0.0	0.0	0.0	0.016204078798120327	0.0
11	0.0	0.0	0.0	0.016204078798120327	0.0
12	0.0	0.0	0.0	0.016204078798120327	0.0
13	0.0	0.0	0.0	0.016204078798120327	0.0
14	0.0	0.0	0.0	0.016204078798120327	0.0
15	0.0	0.0	0.0	0.018518947197851802	0.0
16	0.0	0.0	0.0	0.018518947197851802	0.0
17	0.0	0.0	0.0	0.018518947197851802	0.0
18	0.0	0.0	0.0	0.018518947197851802	0.0
19	0.0	0.0	0.0	0.027778420796777704	0.0
20	0.0	0.0	0.0	0.028935854996643442	0.0
21	0.0	0.0	0.0	0.032408157596240654	0.0
22	0.0	0.0	0.0	0.04861223639436098	0.0
23	0.0	0.0	0.0	0.05092710479409246	0.0
24	0.0	0.0	0.0	0.06134401259288409	0.0
25	0.0	0.0	0.0	0.07176092039167574	0.0
26	0.0	0.0	0.0	0.07407578879140721	0.0
27	0.0	0.0	0.0	0.09375217018912475	0.0
28	0.0	0.0	0.0	0.10648394638764787	0.0
29	0.0	0.0	0.0	0.12963263038496262	0.0
30	0.0	0.0	0.0	0.16435565638093474	0.0
31	0.0	0.0	0.0	0.20370841917636984	0.0
32	0.0	0.0	0.0	0.2430611819718049	0.0
33	0.0	0.0	0.0	0.2766267737679113	0.0
34	0.0	0.0	0.0	0.31945183916294356	0.0
35	0.0	0.0	0.0	0.39005532535475357	0.0
36	0.0	0.0	0.0	0.5000115743419986	0.0
37	0.0	0.0	0.0	0.7176092039167573	0.0
38	0.0	0.0	0.0	0.9328919650917845	0.0
39	0.0	0.0	0.0	1.225722817657816	0.0
40	0.0	0.0	0.0	1.5903145906155236	0.0
41	0.0	0.0	0.0	2.090326164957522	0.0
42	0.0	0.0	0.0	2.562559318502743	0.0
43	0.0	0.0	0.0	3.0938216162411165	0.0
44	0.0	0.0	0.0	3.6424454269774764	0.0
45	0.0	0.0	0.0	4.17370772471585	0.0
46	0.0	0.0	0.0	4.674876733257714	0.0
47	0.0	0.0	0.0	5.176045741799578	0.0
48	0.0	0.0	0.0	5.582305145952453	0.0
49	0.0	0.0	0.0	6.031389615500359	0.0
50	0.0	0.0	0.0	6.547605268640478	0.0
51	0.0	0.0	0.0	7.040672237783282	0.0
52	0.0	0.0	0.0	7.563832496122595	0.0
53	0.0	0.0	0.0	8.112456306858956	0.0
54	0.0	0.0	0.0	8.622884788999745	0.0
55	0.0	0.0	0.0	9.07775642954698	0.0
56	0.0	0.0	0.0	9.5499895830922	0.0
57	0.0	0.0	0.0	9.999074052640108	0.0
58	0.0	0.0	0.0	10.405333456792981	0.0
59	0.0	0.0	0.0	10.800018518947198	0.0
60	0.0	0.0	0.0	11.183129239102756	0.0
61	0.0	0.0	0.0	11.589388643255631	0.0
62	0.0	0.0	0.0	11.919257390217366	0.0
63	0.0	0.0	0.0	12.348665478367554	0.0
64	0.0	0.0	0.0	12.801222250515059	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	20	7.887228E-5	70.00001	42
AGATCTA	20	7.887228E-5	70.00001	40
GTCGCGT	15	0.0022179235	70.0	8
CTTCCGA	25	2.8187897E-6	70.0	18
TTCCGAC	25	2.8187897E-6	70.0	19
AGTCGCG	15	0.0022179235	70.0	7
GACGAAT	45	3.783498E-10	62.222218	23
GCCGTAT	35	2.943616E-7	60.0	54
TAGATCT	95	0.0	55.26316	39
ACGAATA	45	2.6646376E-8	54.444443	24
GATCTCG	85	0.0	53.529408	41
CTGCTGG	20	0.006930391	52.500004	18
CGTATCA	40	7.403287E-7	52.500004	56
GGTCGAT	20	0.006930391	52.500004	7
GAGTCGC	20	0.006930391	52.500004	6
CGTTGTT	20	0.006930391	52.500004	55
CGGCACA	20	0.006930391	52.500004	2
TCTTACG	20	0.006930391	52.500004	11
ATGTCGG	20	0.006930391	52.500004	7
TACGCTG	20	0.006930391	52.500004	14
>>END_MODULE