FastQCFastQC Report
Wed 25 May 2016
SRR1779855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86398
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1947222.53755873978564No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG23542.7246001064839462No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC13011.5058218940253247No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4530.5243176925391791No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT4280.49538183754253573No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4190.48496492974374406RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT3860.4467696011481747No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2000.23148683997314753No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT1810.2094955901756985No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1800.20833815597583277No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1200.13889210398388852No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1190.1377346697840228No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG880.10185420958818492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC150.002209774570.0649354
CCTACCG150.002209774570.0649351
CGTCTTA150.002209774570.0649348
CTTATGC150.002209774570.0649351
CTACCGA150.002209774570.0649352
GCAGTGT150.002219961569.98379524
GACGGCC150.002219961569.98379511
CCTCTCG150.002219961569.98379564
TGCCGGC150.002219961569.98379531
CCGGAAC800.061.23581733
CGGAACT850.057.6337134
CCCAACG252.3677443E-456.05194554
CTTGCAA452.6451744E-854.4949457
CCGTCTT30600.052.9759247
GCCGTCT30650.052.88950346
TCTCGTA28650.052.8847838
CGGATCT28400.052.85746834
TCGTATG30850.052.85647240
CTTGAAA29100.052.8496657
TGCCGTC30800.052.82866745