##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779855_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86398
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.53893608648348	34.0	34.0	34.0	34.0	34.0
2	33.61830134956828	34.0	34.0	34.0	34.0	34.0
3	33.74651033588741	34.0	34.0	34.0	34.0	34.0
4	33.56026759878701	34.0	34.0	34.0	34.0	34.0
5	33.60148383064423	34.0	34.0	34.0	34.0	34.0
6	37.30981041227806	38.0	38.0	38.0	37.0	38.0
7	37.36236949929396	38.0	38.0	38.0	37.0	38.0
8	37.282228755295264	38.0	38.0	38.0	36.0	38.0
9	37.17643926942753	38.0	38.0	38.0	36.0	38.0
10-11	37.136044815852216	38.0	38.0	38.0	35.5	38.0
12-13	37.292176902243106	38.0	38.0	38.0	36.5	38.0
14-15	37.309411097479106	38.0	38.0	38.0	36.5	38.0
16-17	37.166398527743695	38.0	38.0	38.0	36.0	38.0
18-19	37.03475774902197	38.0	38.0	38.0	35.5	38.0
20-21	37.08507141369013	38.0	38.0	38.0	35.5	38.0
22-23	37.09099168962244	38.0	38.0	38.0	36.0	38.0
24-25	37.25745966341813	38.0	38.0	38.0	36.0	38.0
26-27	37.08569642815806	38.0	38.0	38.0	36.0	38.0
28-29	36.59046505706151	38.0	37.0	38.0	34.0	38.0
30-31	37.020949559017566	38.0	37.0	38.0	36.0	38.0
32-33	36.775301511609065	38.0	37.0	38.0	35.0	38.0
34-35	36.90347577490219	38.0	38.0	38.0	35.5	38.0
36-37	36.76521447255723	38.0	37.5	38.0	34.0	38.0
38-39	34.881073635963794	38.0	35.0	38.0	27.5	38.0
40-41	36.332889650223386	38.0	37.0	38.0	33.0	38.0
42-43	37.07249010393759	38.0	37.5	38.0	36.0	38.0
44-45	36.850893539202296	38.0	37.5	38.0	35.0	38.0
46-47	37.011030347924724	38.0	38.0	38.0	36.0	38.0
48-49	36.615558230514594	38.0	37.0	38.0	34.0	38.0
50-51	36.714188985856154	38.0	37.0	38.0	34.5	38.0
52-53	36.622277136044815	38.0	37.0	38.0	34.0	38.0
54-55	36.657243223222764	38.0	37.0	38.0	34.0	38.0
56-57	36.52280724090835	38.0	37.0	38.0	34.0	38.0
58-59	36.56677237899025	38.0	37.0	38.0	34.0	38.0
60-61	36.176757563832496	38.0	37.0	38.0	34.0	38.0
62-63	36.087988147873794	38.0	37.0	38.0	34.0	38.0
64-65	36.559225908007136	38.0	37.0	38.0	34.0	38.0
66-67	36.242910715525824	38.0	37.0	38.0	33.0	38.0
68-69	36.22008611310447	38.0	37.0	38.0	34.0	38.0
70-71	34.410999097201326	38.0	34.0	38.0	26.0	38.0
72-73	31.10068520104632	37.5	23.5	38.0	11.0	38.0
74-75	30.620199541656056	38.0	23.0	38.0	11.0	38.0
76	30.52561401884303	37.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	5.0
24	24.0
25	54.0
26	118.0
27	226.0
28	348.0
29	698.0
30	1064.0
31	1631.0
32	2466.0
33	3905.0
34	6993.0
35	15079.0
36	14869.0
37	38914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.927620150550087	14.797915460335842	11.888824551244934	46.385639837869135
2	13.230630338665247	13.658880992615568	51.95374892937337	21.156739739345817
3	13.383411653047524	16.61844024167226	49.53008171485451	20.468066390425705
4	9.60438899048589	37.35155906386722	30.98567096460566	22.05838098104123
5	9.567351096090187	28.860621773652166	51.16900854186439	10.40301858839325
6	20.304868168244635	52.90515984166299	15.878839787958054	10.91113220213431
7	18.993495219796756	24.4207041829672	17.814069770133568	38.77173082710248
8	16.896224449640037	51.83800550938679	14.04430658117086	17.22146345980231
9	16.009629852542883	15.800134262367186	14.969096506863586	53.22113937822635
10-11	17.682700988448808	32.70851177110581	30.802217643926944	18.80656959651844
12-13	17.234773953100767	17.01775504062594	33.565013079006455	32.18245792726683
14-15	15.85337623556101	16.76890668765481	18.80656959651844	48.57114748026575
16-17	30.622815342947753	31.56323063033867	16.75096645755689	21.062987569156693
18-19	31.521562999143498	21.40385194101715	17.81869950693303	29.255885552906317
20-21	17.012546586726547	18.102270885900136	17.46741822727378	47.41776430009954
22-23	15.881733373457719	32.86071436838816	17.769508553438737	33.48804370471539
24-25	30.29294659598602	32.66973772541031	17.730155790643302	19.30715988796037
26-27	30.890182643116738	34.78147642306535	18.241162989884025	16.087177943933888
28-29	16.6525845505683	34.070811824347786	18.881802819509712	30.394800805574203
30-31	15.733581795874905	20.730803953795228	46.02247737216139	17.513136878168474
32-33	30.359499062478296	18.301928285376974	22.151554434130418	29.187018218014305
34-35	30.323039885182528	18.11095164239913	18.48653904025556	33.07946943216278
36-37	33.537234658209684	30.651172480844462	18.29845598277738	17.513136878168474
38-39	16.75386004305655	21.5930924326952	32.21370865066321	29.439338873585037
40-41	15.559387948795111	17.495196648070557	34.86423296835575	32.08118243477858
42-43	29.9754043809138	17.553748661708962	33.168783818976245	19.302063138400996
44-45	16.211023403319523	31.795874904511678	35.0077548091391	16.9853468830297
46-47	29.722331535452213	18.264311673881338	22.51730364128799	29.496053149378458
48-49	28.60309266418204	17.377138359684253	19.856941132896594	34.16282784323712
50-51	17.417248823628146	17.384258321420095	34.275974232681435	30.922518622270324
52-53	14.454734892336187	17.37207686964575	48.576059272979855	19.597128965038205
54-55	13.916934904939444	17.15142532941204	37.28840052798555	31.643239237662968
56-57	28.63701628030847	17.233852851948775	23.074384567286536	31.05474630045622
58-59	15.584280264658382	17.784556963490804	46.61449137776337	20.016671394087442
60-61	28.269187355649844	30.998454476519044	23.382554686640773	17.349803481190342
62-63	14.634682843812588	49.19249337207822	18.606805052270857	17.566018731838337
64-65	13.748017641543287	50.9081227499508	17.7845046129626	17.559354995543313
66-67	13.750687389655871	50.60056148881363	17.642325837168244	18.006425284362244
68-69	13.672587113912183	50.77868638991614	17.57672563964558	17.972000856526094
70-71	14.135562025038054	48.28304693333025	17.82297412271309	19.758416918918606
72-73	15.347884218020177	42.06386414543939	18.624437846191245	23.963813790349185
74-75	16.18407116523229	38.727059109508566	19.481308708712184	25.60756101654696
76	16.3084117558542	39.22746582398629	19.268210808996308	25.1959116111632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.5
14	3.0
15	8.0
16	7.0
17	3.0
18	9.0
19	24.5
20	34.0
21	34.0
22	62.0
23	121.0
24	183.5
25	215.0
26	218.0
27	247.5
28	341.0
29	408.0
30	501.0
31	678.0
32	859.5
33	957.0
34	1097.0
35	1302.0
36	1615.0
37	1863.0
38	1998.0
39	2404.0
40	2970.5
41	6262.5
42	9259.0
43	10794.0
44	11439.5
45	9117.0
46	7684.0
47	6459.0
48	4650.0
49	3768.5
50	3471.0
51	3119.0
52	2465.5
53	2101.5
54	2039.0
55	1858.5
56	1648.5
57	1360.5
58	1102.0
59	1057.5
60	882.5
61	697.5
62	643.0
63	598.5
64	505.0
65	432.5
66	372.0
67	335.0
68	340.0
69	312.5
70	265.0
71	250.0
72	227.0
73	189.0
74	156.0
75	138.0
76	144.5
77	121.5
78	93.0
79	94.0
80	83.0
81	57.5
82	34.5
83	26.0
84	32.0
85	33.5
86	20.0
87	11.0
88	6.5
89	3.5
90	2.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05555684159355541
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.787170999328688E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.010995624898724507
52-53	0.020833815597583277
54-55	0.037037894395703604
56-57	0.04166763119516655
58-59	0.026042269496979097
60-61	0.02256996689738188
62-63	0.02430611819718049
64-65	0.012731776198523115
66-67	0.023727401097247622
68-69	0.002893585499664344
70-71	0.010995624898724507
72-73	0.013310493298455982
74-75	0.008680756498993032
76	0.008102039399060164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	86398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.710062732933633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.24085437257014	10.437741614389221
2	17.110871703592675	8.45621426421908
3	13.65403531781348	10.121762077825876
4	9.80373787999438	9.690039121275955
5	6.122066607335238	7.563832496122595
6	3.836245257389105	5.687631658140234
7	2.262401049229472	3.9132850297460586
8	1.3724296219963465	2.7130257644852893
9	0.8665511265164645	1.9271279427764532
>10	2.6418099208393837	9.280307414523485
>50	0.03278842100332568	0.5636704553346142
>100	0.04215654128999016	2.877381420866224
>500	0.0	0.0
>1k	0.009368120286664481	4.230422000509271
>5k	0.0	0.0
>10k+	0.0046840601433322405	22.53755873978564
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	19472	22.53755873978564	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	2354	2.7246001064839462	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	1301	1.5058218940253247	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	453	0.5243176925391791	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	428	0.49538183754253573	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	419	0.48496492974374406	RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	386	0.4467696011481747	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	200	0.23148683997314753	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	181	0.2094955901756985	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	180	0.20833815597583277	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	120	0.13889210398388852	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	119	0.1377346697840228	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	88	0.10185420958818492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.017361512997986064	0.0
6	0.0	0.0	0.0	0.017361512997986064	0.0
7	0.0	0.0	0.0	0.017361512997986064	0.0
8	0.0	0.0	0.0	0.017361512997986064	0.0
9	0.0	0.0	0.0	0.020833815597583277	0.0
10	0.0	0.0	0.0	0.020833815597583277	0.0
11	0.0	0.0	0.0	0.021991249797449014	0.0
12	0.0	0.0	0.0	0.021991249797449014	0.0
13	0.0	0.0	0.0	0.021991249797449014	0.0
14	0.0	0.0	0.0	0.021991249797449014	0.0
15	0.0	0.0	0.0	0.02546355239704623	0.0
16	0.0	0.0	0.0	0.02546355239704623	0.0
17	0.0	0.0	0.0	0.02546355239704623	0.0
18	0.0	0.0	0.0	0.02546355239704623	0.0
19	0.0	0.0	0.0	0.03472302599597213	0.0
20	0.0	0.0	0.0	0.038195328595569344	0.0
21	0.0	0.0	0.0	0.04166763119516655	0.0
22	0.0	0.0	0.0	0.04166763119516655	0.0
23	0.0	0.0	0.0	0.042825065395032294	0.0
24	0.0	0.0	0.0	0.042825065395032294	0.0
25	0.0	0.0	0.0	0.053241973193823934	0.0
26	0.0	0.0	0.0	0.05555684159355541	0.0
27	0.0	0.0	0.0	0.07291835459154147	0.0
28	0.0	0.0	0.0	0.08680756498993032	0.0
29	0.0	0.0	0.0	0.11458598578670802	0.0
30	0.0	0.0	0.0	0.15162388018241163	0.0
31	0.0	0.0	0.0	0.19097664297784672	0.0
32	0.0	0.0	0.0	0.2245422347739531	0.0
33	0.0	0.0	0.0	0.26389499756938817	0.0
34	0.0	0.0	0.0	0.3101923655640177	0.0
35	0.0	0.0	0.0	0.38542558855529063	0.0
36	0.0	0.0	0.0	0.5092710479409246	0.0
37	0.0	0.0	0.0	0.7708511771105813	0.0
38	0.0	0.0	0.0	1.0046528854834602	0.0
39	0.0	0.0	0.0	1.305585777448552	0.0
40	0.0	0.0	0.0	1.6817518924049168	0.0
41	0.0	0.0	0.0	2.228060834741545	0.0
42	0.0	0.0	0.0	2.730387277483275	0.0
43	0.0	0.0	0.0	3.281325956619366	0.0
44	0.0	0.0	0.0	3.8496261487534436	0.0
45	0.0	0.0	0.0	4.40982430148846	0.0
46	0.0	0.0	0.0	4.949188638625894	0.0
47	0.0	0.0	0.0	5.483923238963865	0.0
48	0.0	0.0	0.0	5.928377971712308	0.0
49	0.0	0.0	0.0	6.420287506655247	0.0
50	0.0	0.0	0.0	6.964281580592143	0.0
51	0.0	0.0	0.0	7.478182365332531	0.0
52	0.0	0.0	0.0	8.005972360471308	0.0
53	0.0	0.0	0.0	8.565013079006459	0.0
54	0.0	0.0	0.0	9.082386166346444	0.0
55	0.0	0.0	0.0	9.540730109493275	0.0
56	0.0	0.0	0.0	10.018750434037825	0.0
57	0.0	0.0	0.0	10.477094377184658	0.0
58	0.0	0.0	0.0	10.890298386536726	0.0
59	0.0	0.0	0.0	11.287298317090674	0.0
60	0.0	0.0	0.0	11.672723905645965	0.0
61	0.0	0.0	0.0	12.091715085997361	0.0
62	0.0	0.0	0.0	12.43431560915762	0.0
63	0.0	0.0	0.0	12.869510868307136	0.0
64	0.0	0.0	0.0	13.339429153452626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGC	15	0.0022097745	70.06493	54
CCTACCG	15	0.0022097745	70.06493	51
CGTCTTA	15	0.0022097745	70.06493	48
CTTATGC	15	0.0022097745	70.06493	51
CTACCGA	15	0.0022097745	70.06493	52
GCAGTGT	15	0.0022199615	69.983795	24
GACGGCC	15	0.0022199615	69.983795	11
CCTCTCG	15	0.0022199615	69.983795	64
TGCCGGC	15	0.0022199615	69.983795	31
CCGGAAC	80	0.0	61.235817	33
CGGAACT	85	0.0	57.63371	34
CCCAACG	25	2.3677443E-4	56.051945	54
CTTGCAA	45	2.6451744E-8	54.49494	57
CCGTCTT	3060	0.0	52.97592	47
GCCGTCT	3065	0.0	52.889503	46
TCTCGTA	2865	0.0	52.88478	38
CGGATCT	2840	0.0	52.857468	34
TCGTATG	3085	0.0	52.856472	40
CTTGAAA	2910	0.0	52.84966	57
TGCCGTC	3080	0.0	52.828667	45
>>END_MODULE