FastQCFastQC Report
Wed 25 May 2016
SRR1779854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186078
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTC150.002218616770.0266256
ACGTGTA150.002220984570.0077934
GACGAAT950.055.26931423
GGTCCTC200.0069324952.51996656
TAACGTG200.006939867552.50584832
ACGATAT407.4444506E-752.50584824
TGTCCGT200.006939867552.50584835
CAGTCGA200.0069472552.4917379
CGGACTT200.0069472552.49173739
GCCTTAA352.075993E-550.01901660
TCTACGT352.079299E-550.0055768
CGTATCA506.145456E-849.01863556
GTGTCTA305.863413E-446.67186465
GTGTCGA305.863413E-446.67186436
GATATGC451.676839E-646.67186426
GACGATC451.676839E-646.67186423
AGATCTC1350.046.6593240
CGTGCGA305.87119E-446.6593210
ATCTCGG851.8189894E-1245.28698742
ATGCAGT404.5768844E-543.75487529
Produced by FastQC (version 0.11.5)