##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779854_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186078
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.173008093380194	34.0	23.0	34.0	12.0	34.0
2	28.754328829845548	34.0	23.0	34.0	12.0	34.0
3	28.506180204000472	34.0	23.0	34.0	11.0	34.0
4	28.81079439804813	34.0	23.0	34.0	12.0	34.0
5	29.224959425617214	34.0	27.0	34.0	11.0	34.0
6	31.815174281752814	37.0	27.0	38.0	12.0	38.0
7	31.69664334311418	37.0	27.0	38.0	12.0	38.0
8	31.176968798030934	37.0	27.0	38.0	11.0	38.0
9	32.08814583131805	37.0	31.0	38.0	12.0	38.0
10-11	30.70457012650609	36.5	25.5	38.0	11.0	38.0
12-13	31.483007126043915	37.0	27.0	38.0	11.0	38.0
14-15	30.364290781285266	36.5	24.5	38.0	11.0	38.0
16-17	30.926039080385646	36.5	25.0	38.0	11.0	38.0
18-19	31.65516880018057	37.0	27.5	38.0	11.0	38.0
20-21	30.534034114726083	36.5	25.0	38.0	11.0	38.0
22-23	31.33985479207644	37.0	27.0	38.0	11.0	38.0
24-25	31.666846698696247	37.0	27.5	38.0	11.0	38.0
26-27	30.630015369898643	37.0	24.5	38.0	11.0	38.0
28-29	31.69310987865304	37.0	29.0	38.0	11.0	38.0
30-31	32.02715796601426	38.0	31.0	38.0	11.0	38.0
32-33	32.15919668096174	38.0	31.0	38.0	11.0	38.0
34-35	32.10762153505519	38.0	31.0	38.0	11.0	38.0
36-37	31.634989090596417	37.0	26.5	38.0	11.0	38.0
38-39	31.54784821418975	37.0	26.5	38.0	11.0	38.0
40-41	31.54737529423145	37.0	27.0	38.0	11.0	38.0
42-43	31.90521716699449	37.5	29.0	38.0	11.0	38.0
44-45	31.979994948354992	37.0	28.5	38.0	11.0	38.0
46-47	31.8887428927654	37.0	28.5	38.0	11.0	38.0
48-49	31.60266124958351	37.0	27.0	38.0	11.0	38.0
50-51	30.997455368178933	37.0	25.5	38.0	11.0	38.0
52-53	31.561049667343802	37.0	27.0	38.0	11.0	38.0
54-55	31.66841862014854	37.0	28.5	38.0	11.0	38.0
56-57	31.744840873182213	37.0	27.0	38.0	11.0	38.0
58-59	31.959705069916918	37.5	30.5	38.0	11.0	38.0
60-61	31.869146271993465	37.0	27.5	38.0	11.0	38.0
62-63	31.853897290383603	37.0	27.5	38.0	11.0	38.0
64-65	31.858142821827407	37.0	29.0	38.0	11.0	38.0
66-67	31.7849074044218	37.0	27.0	38.0	11.0	38.0
68-69	31.220294715119465	37.0	26.0	38.0	11.0	38.0
70-71	31.281892539687657	37.0	26.0	38.0	11.0	38.0
72-73	31.460341899633487	37.0	27.0	38.0	11.0	38.0
74-75	31.547931512591497	37.0	27.0	38.0	11.0	38.0
76	30.508603918786747	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	35.0
13	563.0
14	2016.0
15	3880.0
16	5214.0
17	6413.0
18	6486.0
19	5705.0
20	4557.0
21	3275.0
22	2434.0
23	2015.0
24	1833.0
25	1860.0
26	1964.0
27	2209.0
28	2581.0
29	3086.0
30	3653.0
31	4643.0
32	5969.0
33	7785.0
34	10771.0
35	15713.0
36	27555.0
37	53858.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.7560915315083	24.36988789647352	9.484194800030094	29.389825771988093
2	18.457851008716773	26.868302539795142	29.769773965756297	24.904072485731792
3	16.443641913606122	22.87051666505444	33.49079418308451	27.195047238254926
4	12.429196358516322	23.151581594815077	30.808048237835745	33.61117380883286
5	12.375992863207902	34.34903642558497	35.329807930007846	17.94516278119928
6	24.88526070273117	42.119801799284154	16.48215226200329	16.512785235981383
7	24.921803622292686	28.971354866448113	19.029934970709945	27.076906540549256
8	22.349950556773724	37.33178554538028	18.349348639236425	21.96891525860957
9	22.516392561539288	19.138449962377727	19.064280339675374	39.28087713640761
10-11	21.720390812257488	27.642497071057754	24.522501800359	26.114610316325766
12-13	21.678870363155138	23.85162096438312	25.970630516854538	28.498878155607205
14-15	24.54790221252492	25.67136185557592	22.091123567125436	27.689612364773726
16-17	25.06153333548297	26.600404131600726	22.59106396242436	25.746998570491947
18-19	21.237152185304115	26.584261510970187	25.167071974633544	27.011514329092158
20-21	23.832478852954136	26.018658841990995	24.463397070045893	25.685465235008976
22-23	25.145169568266645	25.600629851861207	23.34375025191387	25.910450327958273
24-25	21.04572985580773	29.091852034975897	23.420092330347018	26.442325778869357
26-27	22.80232698741116	28.940360871142403	24.435517459123215	23.821794682323226
28-29	21.412159892509237	28.47725898555593	26.101713134027545	24.00886798790729
30-31	22.336063371274413	27.332623953395892	25.543857952041616	24.787454723288082
32-33	20.61022960863081	28.54025876314976	25.221749002431782	25.627762625787643
34-35	20.819673893182284	29.299902189451508	25.226791491557123	24.653632425809086
36-37	23.441132441592018	26.464936398257365	25.032721369809263	25.061209790341348
38-39	23.14164631016589	25.948290457033675	26.578468599929593	24.331594632870846
40-41	21.51689891818978	27.4762598279207	25.95672759126598	25.050113662623534
42-43	19.675994346426478	27.10786394879539	26.701043116559276	26.515098588218855
44-45	19.130217472503556	25.620534262735163	28.232998960066	27.01624930469529
46-47	20.176650149940343	26.341401807882885	27.440426926921546	26.04152111525522
48-49	20.489312248928538	26.651260899356448	27.442866547540675	25.416560304174336
50-51	23.136369743383046	26.36974338304447	25.167808679295984	25.3260781942765
52-53	18.35937814922806	27.815057173185707	26.611128280235945	27.214436397350283
54-55	18.552218591452405	26.479329693008857	26.67657365207671	28.291878063462033
56-57	19.634660245315676	27.101899530223704	26.026627823226512	27.2368124012341
58-59	19.117453406968462	27.408661533706884	25.79110734873221	27.682777710592436
60-61	18.009928319361833	26.473188968653496	26.629343639720805	28.88753907226387
62-63	18.856064860971895	26.563545532477512	26.24106804543708	28.33932156111352
64-65	19.815943170303633	26.017110189512955	25.432525486549178	28.734421153634237
66-67	18.848650174019376	28.91946732601422	23.636534662778665	28.59534783718774
68-69	19.029538926194334	30.0838633572751	23.051809336482577	27.834788380047993
70-71	18.87449516731022	29.855193965922616	23.857660693316994	27.412650173450164
72-73	18.719080773581915	28.52966230360161	23.772031418367867	28.979225504448607
74-75	18.180547829615477	27.70266820379283	23.636808259634584	30.479975706957106
76	18.013360347820477	27.044762002009964	24.32969855916764	30.612179091001916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	3.5
14	5.5
15	6.0
16	14.5
17	21.0
18	30.5
19	38.0
20	56.0
21	76.0
22	85.0
23	133.0
24	235.5
25	299.0
26	404.5
27	562.5
28	745.0
29	875.0
30	1056.5
31	1419.5
32	2010.0
33	2419.0
34	2741.0
35	3537.5
36	4508.5
37	5005.0
38	5720.5
39	7333.0
40	8756.5
41	9821.0
42	10359.0
43	10926.0
44	12030.0
45	12603.5
46	12640.0
47	12753.5
48	12284.5
49	11462.0
50	11222.0
51	10583.0
52	9145.0
53	7774.5
54	7203.0
55	6664.5
56	5602.5
57	4548.5
58	4018.0
59	3592.0
60	2787.0
61	2264.0
62	2120.0
63	1914.0
64	1519.5
65	1236.5
66	1079.5
67	1017.0
68	933.0
69	814.0
70	719.5
71	660.0
72	602.0
73	528.5
74	466.5
75	420.0
76	384.0
77	332.0
78	279.0
79	242.0
80	228.0
81	171.5
82	128.0
83	127.0
84	120.5
85	88.5
86	61.5
87	60.0
88	58.5
89	46.0
90	28.0
91	19.5
92	18.0
93	18.5
94	16.5
95	12.0
96	10.0
97	9.5
98	8.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0021496361740775374
7	0.004299272348155075
8	0.003224454261116306
9	0.009673362783348918
10-11	0.0021496361740775374
12-13	2.687045217596922E-4
14-15	5.374090435193844E-4
16-17	0.0
18-19	0.002955749739356614
20-21	0.0
22-23	0.0013435226087984609
24-25	0.00376186330463569
26-27	2.687045217596922E-4
28-29	0.008329840174550457
30-31	0.0
32-33	2.687045217596922E-4
34-35	0.0021496361740775374
36-37	0.02015283913197691
38-39	0.010479476348627994
40-41	5.374090435193844E-4
42-43	5.374090435193844E-4
44-45	0.0045679768699147675
46-47	0.0021496361740775374
48-49	2.687045217596922E-4
50-51	0.001612227130558153
52-53	0.01101688539214738
54-55	0.00752372660927138
56-57	0.0171970893926203
58-59	0.0137039306097443
60-61	0.023914702436612603
62-63	0.00994206730510861
64-65	0.02606433861069014
66-67	0.019078021044938144
68-69	8.061135652790765E-4
70-71	8.061135652790765E-4
72-73	0.0045679768699147675
74-75	0.00886724921806984
76	0.0026870452175969217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	186078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.638302360594906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59982464097017	47.47198409788513
2	7.4459951501963495	8.881330202858665
3	4.430748501802556	7.927269565027613
4	2.8620457354920714	6.827501957725096
5	1.6863223258260205	5.028470037251692
6	1.0977383672227903	3.9280351594355127
7	0.7028853199181883	2.9343221063873077
8	0.4496440371916605	2.1452805635739867
9	0.3439622750880684	1.8461993546106312
>10	1.3788396627089197	12.941823811617633
>50	0.0019939835831906355	0.06778314362673978
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0021496361740775374	0.0
6	0.0	0.0	0.0	0.0021496361740775374	0.0
7	0.0	0.0	0.0	0.003224454261116306	0.0
8	5.374090435193844E-4	0.0	0.0	0.003224454261116306	0.0
9	5.374090435193844E-4	0.0	0.0	0.005374090435193843	0.0
10	5.374090435193844E-4	0.0	0.0	0.005911499478713228	0.0
11	5.374090435193844E-4	0.0	0.0	0.006448908522232612	0.0
12	5.374090435193844E-4	0.0	0.0	0.008061135652790765	0.0
13	5.374090435193844E-4	0.0	0.0	0.008061135652790765	0.0
14	5.374090435193844E-4	0.0	0.0	0.009673362783348918	0.0
15	5.374090435193844E-4	0.0	0.0	0.010748180870387687	0.0
16	5.374090435193844E-4	0.0	0.0	0.010748180870387687	0.0
17	5.374090435193844E-4	0.0	0.0	0.010748180870387687	0.0
18	5.374090435193844E-4	0.0	0.0	0.010748180870387687	0.0
19	5.374090435193844E-4	0.0	0.0	0.010748180870387687	0.0
20	5.374090435193844E-4	0.0	0.0	0.011822998957426455	0.0
21	5.374090435193844E-4	0.0	0.0	0.01880931652317845	0.0
22	5.374090435193844E-4	0.0	0.0	0.03815604208987629	0.0
23	5.374090435193844E-4	0.0	0.0	0.04406754156858952	0.0
24	5.374090435193844E-4	0.0	0.0	0.04406754156858952	0.0
25	5.374090435193844E-4	0.0	0.0	0.047829404873225206	0.0
26	5.374090435193844E-4	0.0	0.0	0.05105385913434151	0.0
27	5.374090435193844E-4	0.0	0.0	0.06663872139640366	0.0
28	5.374090435193844E-4	0.0	0.0	0.08061135652790766	0.0
29	5.374090435193844E-4	0.0	0.0	0.10801921774739626	0.0
30	5.374090435193844E-4	0.0	0.0	0.157998258794699	0.0
31	5.374090435193844E-4	0.0	0.0	0.19078021044938145	0.0
32	5.374090435193844E-4	0.0	0.0	0.24452111480131988	0.0
33	5.374090435193844E-4	0.0	0.0	0.2907382925439869	0.0
34	5.374090435193844E-4	0.0	0.0	0.33749287933017336	0.0
35	5.374090435193844E-4	0.0	0.0	0.41649200872752284	0.0
36	5.374090435193844E-4	0.0	0.0	0.5529939057814465	0.0
37	5.374090435193844E-4	0.0	0.0	0.8265351089328131	0.0
38	5.374090435193844E-4	0.0	0.0	1.2226055740065993	0.0
39	5.374090435193844E-4	0.0	0.0	1.7342189834370534	0.0
40	5.374090435193844E-4	0.0	0.0	2.2839884349573834	0.0
41	5.374090435193844E-4	0.0	0.0	2.9750964649233116	0.0
42	5.374090435193844E-4	0.0	0.0	3.677490084803147	0.0
43	5.374090435193844E-4	0.0	0.0	4.362686615290362	0.0
44	5.374090435193844E-4	0.0	0.0	5.07152914369243	0.0
45	5.374090435193844E-4	0.0	0.0	5.762099764614839	0.0
46	5.374090435193844E-4	0.0	0.0	6.53811842345683	0.0
47	5.374090435193844E-4	0.0	0.0	7.271681767860789	0.0
48	5.374090435193844E-4	0.0	0.0	7.9751502058276635	0.0
49	5.374090435193844E-4	0.0	0.0	8.657659691097281	0.0
50	5.374090435193844E-4	0.0	0.0	9.30470017949462	0.0
51	5.374090435193844E-4	0.0	0.0	9.963026257805867	0.0
52	5.374090435193844E-4	0.0	0.0	10.713786691602447	0.0
53	5.374090435193844E-4	0.0	0.0	11.505927621750018	0.0
54	5.374090435193844E-4	0.0	0.0	12.258300282677157	0.0
55	5.374090435193844E-4	0.0	0.0	12.983265082384806	0.0
56	5.374090435193844E-4	0.0	0.0	13.567428712690377	0.0
57	5.374090435193844E-4	0.0	0.0	14.225217381958103	0.0
58	5.374090435193844E-4	0.0	0.0	14.783585378174743	0.0
59	5.374090435193844E-4	0.0	0.0	15.380109416481261	0.0
60	5.374090435193844E-4	0.0	0.0	15.908919915304335	0.0
61	5.374090435193844E-4	0.0	0.0	16.46191382108578	0.0
62	5.374090435193844E-4	0.0	0.0	16.964928685819924	0.0
63	5.374090435193844E-4	0.0	0.0	17.550704543256053	0.0
64	5.374090435193844E-4	0.0	0.0	18.096712131471747	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCTC	15	0.0022186167	70.02662	56
ACGTGTA	15	0.0022209845	70.00779	34
GACGAAT	95	0.0	55.269314	23
GGTCCTC	20	0.00693249	52.519966	56
TAACGTG	20	0.0069398675	52.505848	32
ACGATAT	40	7.4444506E-7	52.505848	24
TGTCCGT	20	0.0069398675	52.505848	35
CAGTCGA	20	0.00694725	52.491737	9
CGGACTT	20	0.00694725	52.491737	39
GCCTTAA	35	2.075993E-5	50.019016	60
TCTACGT	35	2.079299E-5	50.00557	68
CGTATCA	50	6.145456E-8	49.018635	56
GTGTCTA	30	5.863413E-4	46.671864	65
GTGTCGA	30	5.863413E-4	46.671864	36
GATATGC	45	1.676839E-6	46.671864	26
GACGATC	45	1.676839E-6	46.671864	23
AGATCTC	135	0.0	46.65932	40
CGTGCGA	30	5.87119E-4	46.65932	10
ATCTCGG	85	1.8189894E-12	45.286987	42
ATGCAGT	40	4.5768844E-5	43.754875	29
>>END_MODULE