FastQCFastQC Report
Wed 25 May 2016
SRR1779854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186078
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2920215.693418888853062No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG36931.9846515977170864No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC20681.1113619019980867No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT7060.37941078472468537Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6670.35845183202742936No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT3450.1854061200141876No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3140.1687464396650867Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT3110.16713421253452854No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2120.11393071722610947Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGT150.00221871670.02582667
ATTGTCG150.00221871670.02582647
GAGGACC150.002225827269.969364
CTGACCG150.002225827269.9693636
ATCGTAT308.374331E-658.30780439
CCGACCA252.3909795E-455.9754944
TCGGCGT200.00691069852.56177553
CGTATTG200.0069180652.5476357
TGCGTAT200.0069180652.5476355
CATTGTC200.006932801652.51936346
CTTTGTC200.006940181452.50524545
GTCCACC200.006947566852.491131
ACGTTTA200.006954957752.4770246
GAACTCG200.006954957752.47702413
GACCGAC200.006954957752.47702438
CTTACGT200.006954957752.4770243
TCTTTAT1450.050.681091
TATGCCG39500.050.39565343
CTGCTTG39300.050.37727454
CGTATGC39600.050.3567441