##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779854_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186078
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.579273208009546	34.0	34.0	34.0	34.0	34.0
2	33.637324132890505	34.0	34.0	34.0	34.0	34.0
3	33.767629703672654	34.0	34.0	34.0	34.0	34.0
4	33.57005664291319	34.0	34.0	34.0	34.0	34.0
5	33.62468964627737	34.0	34.0	34.0	34.0	34.0
6	37.328867464181684	38.0	38.0	38.0	37.0	38.0
7	37.39540407785982	38.0	38.0	38.0	37.0	38.0
8	37.29730005696536	38.0	38.0	38.0	37.0	38.0
9	37.203285718892076	38.0	38.0	38.0	36.0	38.0
10-11	37.165083997033506	38.0	38.0	38.0	36.5	38.0
12-13	37.32085738238803	38.0	38.0	38.0	36.5	38.0
14-15	37.338976128290284	38.0	38.0	38.0	37.0	38.0
16-17	37.248363589462485	38.0	38.0	38.0	36.0	38.0
18-19	37.111077075205024	38.0	38.0	38.0	36.0	38.0
20-21	37.131047195262205	38.0	38.0	38.0	36.0	38.0
22-23	37.13726501789572	38.0	38.0	38.0	36.0	38.0
24-25	37.313231548060486	38.0	38.0	38.0	36.0	38.0
26-27	37.21440471200249	38.0	38.0	38.0	36.0	38.0
28-29	36.73603811304936	38.0	37.0	38.0	34.0	38.0
30-31	37.02016358731285	38.0	37.5	38.0	36.0	38.0
32-33	37.12017003622137	38.0	38.0	38.0	36.0	38.0
34-35	37.01229860596094	38.0	38.0	38.0	36.0	38.0
36-37	36.975749416911185	38.0	38.0	38.0	35.5	38.0
38-39	36.391884048624775	38.0	37.0	38.0	34.0	38.0
40-41	36.85276873139221	38.0	37.0	38.0	35.0	38.0
42-43	37.20849052547857	38.0	38.0	38.0	36.5	38.0
44-45	36.98923032276787	38.0	38.0	38.0	35.5	38.0
46-47	37.11490611464009	38.0	38.0	38.0	36.0	38.0
48-49	36.766431281505604	38.0	37.0	38.0	34.5	38.0
50-51	36.90373123098915	38.0	37.5	38.0	35.0	38.0
52-53	36.84176796827137	38.0	37.5	38.0	35.0	38.0
54-55	36.84835122905448	38.0	37.5	38.0	35.5	38.0
56-57	36.71977611539247	38.0	37.0	38.0	34.5	38.0
58-59	36.79870538161416	38.0	37.0	38.0	35.0	38.0
60-61	36.48421898343705	38.0	37.0	38.0	34.0	38.0
62-63	36.34757736003181	38.0	37.0	38.0	34.0	38.0
64-65	36.74558518470749	38.0	37.0	38.0	34.5	38.0
66-67	36.49505046270919	38.0	37.0	38.0	34.0	38.0
68-69	36.547678930341036	38.0	37.0	38.0	34.0	38.0
70-71	35.34377787809413	38.0	36.5	38.0	28.5	38.0
72-73	33.13598329732693	38.0	33.5	38.0	16.0	38.0
74-75	32.797657433979296	38.0	33.0	38.0	11.0	38.0
76	32.634454368598114	38.0	32.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	3.0
23	9.0
24	32.0
25	73.0
26	162.0
27	350.0
28	542.0
29	929.0
30	1535.0
31	2564.0
32	4093.0
33	6670.0
34	11479.0
35	24336.0
36	33305.0
37	99993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.17040908504323	15.871402761646664	11.448251387275779	39.50993676603433
2	16.57530712926837	15.627854985543696	45.19663796902375	22.60019991616419
3	15.072711443588172	18.15582712625888	42.34998226550156	24.421479164651384
4	11.05826588849837	29.955180085770483	33.92663291737873	25.059921108352412
5	10.80783327421834	30.514622900074162	46.48856930964435	12.188974516063155
6	22.88126484592483	48.29211405969539	16.910112963380946	11.91650813099883
7	23.293994991347713	26.408280398542544	18.921097604230482	31.376627005879254
8	20.010425735444276	45.98071776351853	15.593460806758456	18.415395694278743
9	19.302658025129247	15.724051204333666	16.790808155719645	48.18248261481744
10-11	19.981943056137748	30.288642397274263	29.690237427315424	20.039177119272562
12-13	19.757574780468406	19.03046034458668	31.185578090908113	30.026386784036802
14-15	18.30307720418319	18.947161942841173	20.658272337406896	42.09148851556874
16-17	27.86304667934952	30.314169326841434	19.951579445178904	21.871204548630143
18-19	28.601178000623396	23.345854964047337	20.7222240135857	27.33074302174357
20-21	18.51723470802567	21.22174571953697	20.80982168767936	39.451197884758
22-23	18.40948419480003	30.995603994024012	20.833198981072453	29.761712830103505
24-25	27.694523488995117	30.813133308074903	20.863462070707477	20.6288811322225
26-27	28.09010200023646	32.46702995518009	20.94793581186384	18.494932232719613
28-29	18.68651855673427	31.94010038800933	21.926288975590882	27.44709207966552
30-31	27.657756424725115	23.458173454142887	29.912187362288932	18.971882758843066
32-33	26.873407925708577	31.537581014413313	23.740581906512322	17.848429153365792
34-35	20.37344554434162	31.443803136319175	30.327604552929415	17.855146766409785
36-37	17.897602080847815	30.522684035726954	33.43141048377562	18.14830339964961
38-39	17.691774417179893	23.281903287868527	32.0196369264502	27.006685368501383
40-41	17.836606154408365	20.027085415793376	32.79296854007459	29.343339889723662
42-43	27.43602765529231	20.144402974045622	31.641018613312266	20.7785507573498
44-45	17.91264953406636	29.754189103494234	33.143627940970994	19.189533421468415
46-47	26.832833542922863	20.773009168198282	24.777243951461216	27.616913337417643
48-49	25.78757295327766	19.97952471544191	23.526155698148088	30.706746633132344
50-51	18.30778864423679	20.212151263259454	33.024539564033724	28.455520528470036
52-53	15.936867512626229	20.149821391613333	42.373059028123095	21.540252067637343
54-55	15.383808837604448	20.598135263310713	34.63904332770543	29.37901257137941
56-57	25.013240849027436	20.29412160073127	25.23127797717466	29.461359573066638
58-59	16.411752056341054	20.441642922423526	40.842159023708405	22.304445997527015
60-61	25.146428331429338	29.702304952624154	25.00288959075331	20.1483771251932
62-63	15.769852085399648	41.70906813532989	21.795833232537447	20.725246546733004
64-65	15.525025799793601	42.77767027863777	20.786033711730305	20.91127020983832
66-67	15.320638265843744	42.86708887980903	20.253059073998408	21.55921378034881
68-69	15.544137525159826	43.181384549649174	20.014457662964467	21.260020262226533
70-71	16.172708118335052	41.29310715514276	20.125182748538013	22.409001977984175
72-73	17.03587036569174	37.46337975928743	20.20550338384463	25.295246491176204
74-75	17.555038376367897	35.77575086534947	20.832347945735602	25.83686281254703
76	17.92035517143149	36.13149082778378	20.62122751288625	25.326926487898476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	5.0
15	8.0
16	13.0
17	18.0
18	34.5
19	51.5
20	54.5
21	57.0
22	86.0
23	157.0
24	299.5
25	400.0
26	444.5
27	562.0
28	797.0
29	959.0
30	1080.5
31	1378.0
32	1825.0
33	2096.0
34	2469.0
35	3287.5
36	4230.0
37	4727.0
38	9861.5
39	17602.0
40	19013.5
41	15884.5
42	13950.0
43	12751.0
44	11199.0
45	10415.5
46	9985.0
47	9957.5
48	9286.5
49	8602.0
50	8561.0
51	8086.0
52	6596.0
53	5218.0
54	4855.0
55	4512.0
56	3768.5
57	2937.5
58	2507.0
59	2179.5
60	1712.5
61	1439.0
62	1305.0
63	1152.0
64	914.5
65	809.5
66	708.0
67	627.0
68	579.5
69	548.0
70	522.5
71	481.0
72	484.0
73	505.5
74	446.0
75	368.0
76	357.0
77	323.0
78	246.5
79	193.0
80	178.0
81	142.0
82	98.0
83	75.0
84	80.5
85	79.0
86	48.0
87	24.0
88	23.5
89	16.5
90	6.5
91	2.0
92	1.0
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0548157224389772
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.061135652790765E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.061135652790765E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.016928384870860607
52-53	0.030363610958845218
54-55	0.053740904351938436
56-57	0.05400960887369813
58-59	0.03654381495931814
60-61	0.03520029235051968
62-63	0.03143842904588398
64-65	0.01612227130558153
66-67	0.03976826922043444
68-69	0.009404658261589226
70-71	0.01612227130558153
72-73	0.025258225045411063
74-75	0.013972635131503992
76	0.014510044175023377
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	186078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.35371188426359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.02551856274123	7.165812186287471
2	12.853691722433567	4.97533292490246
3	11.431982895065671	6.637539096507917
4	10.11023796962208	7.826825309816314
5	7.425096492933108	7.1851589118541686
6	5.4147113542331935	6.287685809176796
7	3.706994696359648	5.022087511688646
8	2.4963207730541748	3.8650458409914124
9	1.5688779052008999	2.7327249862960694
>10	7.761086274400911	25.640860284396865
>50	0.15827617804681643	1.954556691280001
>100	0.03332130064143504	1.1790754414815292
>500	0.005553550106905839	0.7378626167521147
>1k	0.005553550106905839	3.0960134997151734
>5k	0.0	0.0
>10k+	0.0027767750534529196	15.693418888853062
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	29202	15.693418888853062	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	3693	1.9846515977170864	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	2068	1.1113619019980867	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	706	0.37941078472468537	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	667	0.35845183202742936	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT	345	0.1854061200141876	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	314	0.1687464396650867	Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT	311	0.16713421253452854	No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	212	0.11393071722610947	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0026870452175969217	0.0
6	0.0	0.0	0.0	0.0026870452175969217	0.0
7	0.0	0.0	0.0	0.00376186330463569	0.0
8	0.0	0.0	0.0	0.00376186330463569	0.0
9	0.0	0.0	0.0	0.005911499478713228	0.0
10	0.0	0.0	0.0	0.006448908522232612	0.0
11	0.0	0.0	0.0	0.00752372660927138	0.0
12	0.0	0.0	0.0	0.009135953739829533	0.0
13	0.0	0.0	0.0	0.009135953739829533	0.0
14	0.0	0.0	0.0	0.011285589913907072	0.0
15	0.0	0.0	0.0	0.015584862262062146	0.0
16	0.0	0.0	0.0	0.015584862262062146	0.0
17	0.0	0.0	0.0	0.015584862262062146	0.0
18	0.0	0.0	0.0	0.015584862262062146	0.0
19	0.0	0.0	0.0	0.015584862262062146	0.0
20	0.0	0.0	0.0	0.01612227130558153	0.0
21	0.0	0.0	0.0	0.017734498436139685	0.0
22	0.0	0.0	0.0	0.018271907479659066	0.0
23	0.0	0.0	0.0	0.026333043132449833	0.0
24	0.0	0.0	0.0	0.026333043132449833	0.0
25	0.0	0.0	0.0	0.031169724524124292	0.0
26	0.0	0.0	0.0	0.0343941787852406	0.0
27	0.0	0.0	0.0	0.051591268177860895	0.0
28	0.0	0.0	0.0	0.06663872139640366	0.0
29	0.0	0.0	0.0	0.09727103687700857	0.0
30	0.0	0.0	0.0	0.1472500779243113	0.0
31	0.0	0.0	0.0	0.18433130192714883	0.0
32	0.0	0.0	0.0	0.23914702436612603	0.0
33	0.0	0.0	0.0	0.29342533776158386	0.0
34	0.0	0.0	0.0	0.3498532873311192	0.0
35	0.0	0.0	0.0	0.43422650716366257	0.0
36	0.0	0.0	0.0	0.583088812218532	0.0
37	0.0	0.0	0.0	0.892099012242178	0.0
38	0.0	0.0	0.0	1.3107406571437785	0.0
39	0.0	0.0	0.0	1.8422382011844496	0.0
40	0.0	0.0	0.0	2.4387622394909663	0.0
41	0.0	0.0	0.0	3.179849310504197	0.0
42	0.0	0.0	0.0	3.9311471533442965	0.0
43	0.0	0.0	0.0	4.672234224357528	0.0
44	0.0	0.0	0.0	5.425144294328185	0.0
45	0.0	0.0	0.0	6.164081729167338	0.0
46	0.0	0.0	0.0	6.9868549747955155	0.0
47	0.0	0.0	0.0	7.755887316071755	0.0
48	0.0	0.0	0.0	8.50127365943314	0.0
49	0.0	0.0	0.0	9.231075140532464	0.0
50	0.0	0.0	0.0	9.923257988585432	0.0
51	0.0	0.0	0.0	10.612216382377282	0.0
52	0.0	0.0	0.0	11.39145949548039	0.0
53	0.0	0.0	0.0	12.199722696933543	0.0
54	0.0	0.0	0.0	12.959081675426434	0.0
55	0.0	0.0	0.0	13.702855791657262	0.0
56	0.0	0.0	0.0	14.287019421962833	0.0
57	0.0	0.0	0.0	14.97006631627597	0.0
58	0.0	0.0	0.0	15.535420630058363	0.0
59	0.0	0.0	0.0	16.153978439149174	0.0
60	0.0	0.0	0.0	16.689775255538	0.0
61	0.0	0.0	0.0	17.258891432625028	0.0
62	0.0	0.0	0.0	17.778028568664755	0.0
63	0.0	0.0	0.0	18.385300787841658	0.0
64	0.0	0.0	0.0	18.960865873450917	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAGT	15	0.002218716	70.025826	67
ATTGTCG	15	0.002218716	70.025826	47
GAGGACC	15	0.0022258272	69.96936	4
CTGACCG	15	0.0022258272	69.96936	36
ATCGTAT	30	8.374331E-6	58.307804	39
CCGACCA	25	2.3909795E-4	55.975494	4
TCGGCGT	20	0.006910698	52.561775	53
CGTATTG	20	0.00691806	52.54763	57
TGCGTAT	20	0.00691806	52.54763	55
CATTGTC	20	0.0069328016	52.519363	46
CTTTGTC	20	0.0069401814	52.505245	45
GTCCACC	20	0.0069475668	52.49113	1
ACGTTTA	20	0.0069549577	52.477024	6
GAACTCG	20	0.0069549577	52.477024	13
GACCGAC	20	0.0069549577	52.477024	38
CTTACGT	20	0.0069549577	52.477024	3
TCTTTAT	145	0.0	50.68109	1
TATGCCG	3950	0.0	50.395653	43
CTGCTTG	3930	0.0	50.377274	54
CGTATGC	3960	0.0	50.35674	41
>>END_MODULE