FastQCFastQC Report
Wed 25 May 2016
SRR1779853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181262
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAT150.002223193669.989521
TCGAACG150.002223193669.9895212
ATCTCGT252.3874146E-455.99161542
GACGTCG200.00694675252.49213814
TACCTGG200.00694675252.49213845
ACGTCGC200.00694675252.49213815
TATCGCA200.00694675252.49213846
CGAAGCT200.00694675252.49213813
CGTTGTT305.862583E-446.67255855
ACGTGTA451.6764261E-646.67255434
CGGTGGT900.046.65968346
TCTCGTT305.870565E-446.65967643
TCGGTGG1350.046.65967645
CTCGGTG1450.045.855244
GACGAAT404.576015E-543.75552423
AGATCTC1550.042.896840
GACGATC503.4616205E-642.005323
GATCTCG1350.041.47526641
TGGACGC350.001253895839.9940149
GGTGGTC1050.039.9940147
Produced by FastQC (version 0.11.5)