##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779853_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181262
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.729270337963833	34.0	23.0	34.0	12.0	34.0
2	29.1541801370392	34.0	27.0	34.0	12.0	34.0
3	28.915382154009112	34.0	23.0	34.0	11.0	34.0
4	29.055510807560328	34.0	24.0	34.0	12.0	34.0
5	29.677284814246782	34.0	31.0	34.0	11.0	34.0
6	31.963621718837924	37.0	28.0	38.0	12.0	38.0
7	32.30129315576348	37.0	31.0	38.0	12.0	38.0
8	31.463621718837924	37.0	27.0	38.0	11.0	38.0
9	32.469706833202764	37.0	31.0	38.0	12.0	38.0
10-11	31.01243503878364	36.5	26.0	38.0	11.0	38.0
12-13	31.840424358111463	37.0	28.0	38.0	11.0	38.0
14-15	30.704469221348106	36.5	25.5	38.0	11.0	38.0
16-17	31.304076419768073	37.0	28.0	38.0	11.0	38.0
18-19	32.1083735145811	37.0	31.0	38.0	11.0	38.0
20-21	30.889251470247487	36.5	25.5	38.0	11.0	38.0
22-23	31.769761450276395	37.0	28.5	38.0	11.0	38.0
24-25	32.07509571780075	37.0	29.5	38.0	11.0	38.0
26-27	30.98579956085666	37.0	25.5	38.0	11.0	38.0
28-29	32.133938718540016	37.0	29.5	38.0	11.0	38.0
30-31	32.55425847668016	38.0	32.0	38.0	11.0	38.0
32-33	32.670479747547745	38.0	33.0	38.0	11.0	38.0
34-35	32.58933753351502	38.0	32.0	38.0	11.0	38.0
36-37	32.127012280566255	37.0	29.0	38.0	11.0	38.0
38-39	32.04190343260032	37.0	29.0	38.0	11.0	38.0
40-41	32.03298816078384	37.0	29.0	38.0	11.0	38.0
42-43	32.40964736127815	37.5	32.5	38.0	11.0	38.0
44-45	32.472925930421155	37.5	31.0	38.0	11.0	38.0
46-47	32.34792179276407	37.5	31.0	38.0	11.0	38.0
48-49	32.06317099005859	37.0	29.0	38.0	11.0	38.0
50-51	31.417679932914787	37.0	28.0	38.0	11.0	38.0
52-53	31.999263497037436	37.0	29.0	38.0	11.0	38.0
54-55	32.13151680992155	37.0	30.0	38.0	11.0	38.0
56-57	32.195360858867275	37.0	31.0	38.0	11.0	38.0
58-59	32.452461078438944	37.5	31.5	38.0	11.0	38.0
60-61	32.3326041862056	37.0	31.0	38.0	11.0	38.0
62-63	32.31737484966513	37.0	31.0	38.0	11.0	38.0
64-65	32.32724454105107	37.5	31.0	38.0	11.0	38.0
66-67	32.2169870132736	37.0	31.0	38.0	11.0	38.0
68-69	31.612789773918415	37.0	27.0	38.0	11.0	38.0
70-71	31.612533239178646	37.0	27.5	38.0	11.0	38.0
72-73	31.844396508920788	37.0	29.0	38.0	11.0	38.0
74-75	31.944872615330297	37.0	29.5	38.0	11.0	38.0
76	30.798380245169977	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	32.0
13	422.0
14	1651.0
15	3044.0
16	4302.0
17	5195.0
18	5409.0
19	4843.0
20	3829.0
21	2941.0
22	2293.0
23	1990.0
24	1827.0
25	2056.0
26	2131.0
27	2403.0
28	2827.0
29	3454.0
30	4118.0
31	5084.0
32	6333.0
33	8229.0
34	11082.0
35	16092.0
36	27911.0
37	51758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57926967190957	18.652109389223277	9.831679180849715	29.936941758017444
2	20.07811896591674	20.476437422074127	30.509428341296026	28.93601527071311
3	17.9740815729804	20.685089456639876	32.9668268408538	28.374002129525934
4	13.296774834217873	24.37355871611259	30.229722721806006	32.09994372786353
5	13.888183954717482	33.063190299125026	34.88210435722876	18.166521388928732
6	26.326997875920664	37.21718021571819	16.650575156547408	19.805246751813744
7	25.83930571417537	30.96369123480698	18.635483390436466	24.561519660581183
8	22.612643925475982	35.83585737378419	17.0713406930491	24.48015800769074
9	23.31718567235329	17.893557855708327	18.716757520248947	40.07249895168943
10-11	22.710847029356106	26.789310205950667	23.75246197388239	26.747380790810837
12-13	22.662010410373963	22.871928125939593	25.664026834159486	28.802034629526958
14-15	24.688429264202053	24.915452474084045	21.88911998852471	28.506998273189193
16-17	25.489076464746773	26.0948361469712	22.50551693699658	25.910570451285448
18-19	22.31340669162209	25.808453765795985	24.89538306212467	26.982756480457255
20-21	24.291908949476447	25.104820646357208	24.368041839988525	26.23522856417782
22-23	25.632665221205247	25.132270753681237	23.294272772913597	25.94079125219991
24-25	22.06305463834201	28.270038702273865	22.962337694580302	26.704568964803826
26-27	23.397946038690385	28.190798275400734	23.862473415186514	24.548782270722363
28-29	22.257961291461612	27.41349060975879	25.452684660031338	24.87586343874826
30-31	22.81323616221912	26.62017753405311	25.226882782286314	25.339703521441457
32-33	21.496231973607266	27.758438062031754	25.161092782822653	25.584237181538327
34-35	21.48321016465707	28.662026807462393	24.94434651638157	24.910416511498962
36-37	24.00086077988865	25.963549282407534	24.762044021166357	25.27354591653746
38-39	23.713982261824302	25.367522310652564	26.0342901281397	24.88420529938344
40-41	22.087977866231938	26.45818334284997	25.564171100868084	25.88966769005001
42-43	20.492054253408767	26.322061342653253	26.359852256834778	26.826032147103202
44-45	20.058812332935183	24.385049750488953	28.32619328071855	27.22994463585732
46-47	20.773427931456816	25.009103046486224	27.65891712365799	26.558551898398967
48-49	20.84027540245611	25.712780395229007	27.143582218004873	26.30336198431
50-51	22.7653153550779	25.84245487045946	25.72273469567904	25.669495078783598
52-53	18.521696189890285	27.13810026952988	26.885398763528222	27.45480477705161
54-55	18.972570807482544	25.97290460334844	26.788083763451837	28.266440825717176
56-57	19.6840618413357	26.677352431609265	25.990962160253368	27.647623566801666
58-59	19.438252463611384	26.459682847967862	26.099934892241137	28.00212979617961
60-61	18.700286109218126	25.567735046861767	26.526214722623042	29.20576412129707
62-63	19.50674501365555	25.792159783718173	25.905266352175232	28.79582885045105
64-65	20.203672843253027	25.297425257419075	25.118639017337852	29.380262881990042
66-67	19.55359975721124	27.543557131230084	23.830544481383896	29.07229863017478
68-69	19.728127594662958	28.505225875323774	22.95192305039956	28.814723479613708
70-71	19.58667336051329	28.4912363939292	23.798555673862552	28.123534571694957
72-73	19.31162708582179	27.572708940792868	23.765610043392265	29.350053929993074
74-75	19.279873762731313	26.908180042594044	23.220374518610065	30.59157167606458
76	18.343567420844437	26.342155061597623	23.721566615358302	31.592710902199638
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	1.5
14	3.5
15	8.0
16	13.0
17	15.0
18	26.5
19	57.0
20	100.0
21	124.0
22	137.0
23	201.5
24	315.5
25	378.0
26	438.0
27	645.5
28	863.5
29	934.0
30	1139.5
31	1550.0
32	2088.0
33	2421.0
34	2779.5
35	3567.5
36	4377.5
37	4758.0
38	5131.5
39	6246.5
40	7568.5
41	8638.5
42	9128.0
43	9429.0
44	10124.0
45	10586.0
46	10654.0
47	10855.5
48	10785.5
49	10211.0
50	9908.0
51	9518.0
52	8698.0
53	7845.5
54	7423.0
55	6938.0
56	5969.5
57	5083.5
58	4681.0
59	4295.5
60	3633.0
61	3044.5
62	2733.0
63	2562.0
64	2232.5
65	1902.5
66	1653.0
67	1575.0
68	1473.5
69	1266.0
70	1097.0
71	1034.0
72	959.0
73	825.5
74	741.0
75	715.0
76	670.0
77	593.5
78	497.5
79	433.0
80	400.0
81	343.5
82	282.0
83	244.0
84	211.0
85	156.0
86	128.5
87	123.0
88	97.0
89	71.0
90	51.5
91	32.5
92	33.0
93	28.5
94	19.0
95	14.5
96	15.0
97	13.5
98	11.5
99	9.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.51687612406351E-4
2	0.0
3	5.51687612406351E-4
4	0.0
5	0.0
6	0.0038618132868444574
7	0.006068563736469862
8	0.002758438062031755
9	0.009930377023314318
10-11	0.002758438062031755
12-13	2.758438062031755E-4
14-15	0.0016550628372190529
16-17	0.001103375224812702
18-19	0.003034281868234931
20-21	0.0
22-23	0.002758438062031755
24-25	0.003585969480641282
26-27	0.0013792190310158775
28-29	0.00551687612406351
30-31	5.51687612406351E-4
32-33	0.0
34-35	0.003585969480641282
36-37	0.01710231598459688
38-39	0.00799947037989209
40-41	2.758438062031755E-4
42-43	8.275314186095264E-4
44-45	0.004137657093047633
46-47	0.002206750449625404
48-49	0.0
50-51	0.0033101256744381058
52-53	0.011309596054330196
54-55	0.007447782767485739
56-57	0.013240502697752423
58-59	0.013240502697752423
60-61	0.020688285465238165
62-63	0.009378689410907968
64-65	0.02206750449625404
66-67	0.01903322262801911
68-69	2.758438062031755E-4
70-71	5.51687612406351E-4
72-73	0.004689344705453984
74-75	0.008827001798501617
76	0.002758438062031755
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	181262.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.9893473277098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30339326822345	48.82604334123184
2	10.606557117978623	13.574133347470536
3	5.942614990444983	11.407921639753168
4	3.220881601667245	8.244084460420623
5	1.6108403141623195	5.153831017620493
6	0.7954966782549526	3.0541987945737348
7	0.4964923740040207	2.2239156075991735
8	0.2601529032899755	1.3317611589547471
9	0.20057421785919863	1.1551151962418398
>10	0.5629965341152297	5.028995436133836
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.51687612406351E-4	0.0
2	0.0	0.0	0.0	5.51687612406351E-4	0.0
3	0.0	0.0	0.0	5.51687612406351E-4	0.0
4	0.0	0.0	0.0	0.004413500899250808	0.0
5	0.0	0.0	0.0	0.006068563736469862	0.0
6	0.0	0.0	0.0	0.006068563736469862	0.0
7	0.0	0.0	0.0	0.008275314186095265	0.0
8	0.0	0.0	0.0	0.008275314186095265	0.0
9	0.0	0.0	0.0	0.009930377023314318	0.0
10	0.0	0.0	0.0	0.012688815085346073	0.0
11	0.0	0.0	0.0	0.013240502697752425	0.0
12	0.0	0.0	0.0	0.013792190310158776	0.0
13	0.0	0.0	0.0	0.013792190310158776	0.0
14	0.0	0.0	0.0	0.018757378821815936	0.0
15	0.0	0.0	0.0	0.019860754046628636	0.0
16	0.0	0.0	0.0	0.02151581688384769	0.0
17	0.0	0.0	0.0	0.02206750449625404	0.0
18	0.0	0.0	0.0	0.023170879721066743	0.0
19	0.0	0.0	0.0	0.024825942558285796	0.0
20	0.0	0.0	0.0	0.02648100539550485	0.0
21	0.0	0.0	0.0	0.02648100539550485	0.0
22	0.0	0.0	0.0	0.03310125674438106	0.0
23	0.0	0.0	0.0	0.034204631969193766	0.0
24	0.0	0.0	0.0	0.04358332138010173	0.0
25	0.0	0.0	0.0	0.046893447054539836	0.0
26	0.0	0.0	0.0	0.05351369840341605	0.0
27	0.0	0.0	0.0	0.0799947037989209	0.0
28	0.0	0.0	0.0	0.11144089770608291	0.0
29	0.0	0.0	0.0	0.14288709161324492	0.0
30	0.0	0.0	0.0	0.1815052244816895	0.0
31	0.0	0.0	0.0	0.22233010779975945	0.0
32	0.0	0.0	0.0	0.25653473976895325	0.0
33	0.0	0.0	0.0	0.3117035010095883	0.0
34	0.0	0.0	0.0	0.36797563747503614	0.0
35	0.0	0.0	0.0	0.49100197504165244	0.0
36	0.0	0.0	0.0	0.6807825137094372	0.0
37	0.0	0.0	0.0	0.9974512032306827	0.0
38	0.0	0.0	0.0	1.4575586719775795	0.0
39	0.0	0.0	0.0	2.0109013472211497	0.0
40	0.0	0.0	0.0	2.629343160728669	0.0
41	0.0	0.0	0.0	3.485562335183326	0.0
42	0.0	0.0	0.0	4.478600037514758	0.0
43	0.0	0.0	0.0	5.4263993556288685	0.0
44	0.0	0.0	0.0	6.298617470843309	0.0
45	0.0	0.0	0.0	7.2629674173296115	0.0
46	0.0	0.0	0.0	8.210766735443721	0.0
47	0.0	0.0	0.0	9.108362480828855	0.0
48	0.0	0.0	0.0	9.817832750383422	0.0
49	0.0	0.0	0.0	10.561507651907185	0.0
50	0.0	0.0	0.0	11.252772230252342	0.0
51	0.0	0.0	0.0	12.021824761946796	0.0
52	0.0	0.0	0.0	12.972934205735344	0.0
53	0.0	0.0	0.0	13.891494080391919	0.0
54	0.0	0.0	0.0	14.83763833566881	0.0
55	0.0	0.0	0.0	15.643102249782084	0.0
56	0.0	0.0	0.0	16.36029614591034	0.0
57	0.0	0.0	0.0	17.065904602178062	0.0
58	0.0	0.0	0.0	17.67993291478633	0.0
59	0.0	0.0	0.0	18.26417009632466	0.0
60	0.0	0.0	0.0	18.81420264589379	0.0
61	0.0	0.0	0.0	19.392371263695644	0.0
62	0.0	0.0	0.0	19.994262448830973	0.0
63	0.0	0.0	0.0	20.66897639880394	0.0
64	0.0	0.0	0.0	21.31169246725734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCTAT	15	0.0022231936	69.98952	1
TCGAACG	15	0.0022231936	69.98952	12
ATCTCGT	25	2.3874146E-4	55.991615	42
GACGTCG	20	0.006946752	52.492138	14
TACCTGG	20	0.006946752	52.492138	45
ACGTCGC	20	0.006946752	52.492138	15
TATCGCA	20	0.006946752	52.492138	46
CGAAGCT	20	0.006946752	52.492138	13
CGTTGTT	30	5.862583E-4	46.672558	55
ACGTGTA	45	1.6764261E-6	46.672554	34
CGGTGGT	90	0.0	46.659683	46
TCTCGTT	30	5.870565E-4	46.659676	43
TCGGTGG	135	0.0	46.659676	45
CTCGGTG	145	0.0	45.8552	44
GACGAAT	40	4.576015E-5	43.755524	23
AGATCTC	155	0.0	42.8968	40
GACGATC	50	3.4616205E-6	42.0053	23
GATCTCG	135	0.0	41.475266	41
TGGACGC	35	0.0012538958	39.99401	49
GGTGGTC	105	0.0	39.99401	47
>>END_MODULE