FastQCFastQC Report
Wed 25 May 2016
SRR1779853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181262
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2353112.981761207533845No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG28801.5888603237302912No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC16810.9273868764550761No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT6010.331564255056217RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4840.2670168044046739No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3540.19529741479184826No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT3480.19198728911741014No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1950.10757908441923844No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG150.00221081170.088121
CGTATGA150.00222295169.9914541
GTATGAC150.00222295169.9914542
AACTCGT553.6379788E-1157.2657337
CGTCTTA252.3838355E-456.0086148
GCTTGAC252.3838355E-456.0086156
ATCTCGT33650.053.7675837
CGTCTTC34250.053.65788348
CTCGTAT34850.053.62328739
TCTCGTA33800.053.52896538
CATCTCG33750.053.50457436
CCGTCTT34550.053.49592247
TATGCCG34700.053.45168343
TCGTATG35100.053.44076540
GTCTTCT34550.053.39460449
TCTTCTG34500.053.3705250
CTGCTTG34400.053.3221554
CGTATGC35050.053.3173141
TGCCGTC34750.053.2887745
GCCGTCT34700.053.2646746