##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779853_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181262
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.54791406913749	34.0	34.0	34.0	34.0	34.0
2	33.62234224492723	34.0	34.0	34.0	34.0	34.0
3	33.76079376813673	34.0	34.0	34.0	34.0	34.0
4	33.562622060884244	34.0	34.0	34.0	34.0	34.0
5	33.6097251492315	34.0	34.0	34.0	34.0	34.0
6	37.303025454866436	38.0	38.0	38.0	37.0	38.0
7	37.364466904260134	38.0	38.0	38.0	37.0	38.0
8	37.24838079685759	38.0	38.0	38.0	36.0	38.0
9	37.15454425086339	38.0	38.0	38.0	36.0	38.0
10-11	37.10998168397127	38.0	38.0	38.0	35.5	38.0
12-13	37.27744094184109	38.0	38.0	38.0	36.5	38.0
14-15	37.29700102613896	38.0	38.0	38.0	36.5	38.0
16-17	37.212101819465744	38.0	38.0	38.0	36.0	38.0
18-19	37.07757279518046	38.0	38.0	38.0	35.5	38.0
20-21	37.089014796261765	38.0	38.0	38.0	36.0	38.0
22-23	37.08801900012138	38.0	38.0	38.0	36.0	38.0
24-25	37.26167095144045	38.0	38.0	38.0	36.0	38.0
26-27	37.175544239829634	38.0	38.0	38.0	36.0	38.0
28-29	36.659512197813115	38.0	37.0	38.0	34.0	38.0
30-31	36.925108406615834	38.0	37.0	38.0	35.5	38.0
32-33	37.04602729750306	38.0	38.0	38.0	36.0	38.0
34-35	37.1023435689775	38.0	38.0	38.0	36.0	38.0
36-37	37.044101907735765	38.0	38.0	38.0	36.0	38.0
38-39	36.305477706303584	38.0	37.0	38.0	34.0	38.0
40-41	36.77167028941532	38.0	37.0	38.0	35.0	38.0
42-43	37.1594984056228	38.0	38.0	38.0	36.0	38.0
44-45	36.92062042788891	38.0	37.5	38.0	35.5	38.0
46-47	37.069611942933435	38.0	38.0	38.0	36.0	38.0
48-49	36.69849996138187	38.0	37.0	38.0	34.5	38.0
50-51	36.84982511502687	38.0	37.0	38.0	35.0	38.0
52-53	36.78578245854067	38.0	37.0	38.0	35.0	38.0
54-55	36.80379505908574	38.0	37.0	38.0	35.0	38.0
56-57	36.67934812591719	38.0	37.0	38.0	34.5	38.0
58-59	36.75332943474088	38.0	37.0	38.0	34.5	38.0
60-61	36.403840849157575	38.0	37.0	38.0	34.0	38.0
62-63	36.23743531462745	38.0	37.0	38.0	34.0	38.0
64-65	36.644829032008914	38.0	37.0	38.0	34.0	38.0
66-67	36.40744061082853	38.0	37.0	38.0	34.0	38.0
68-69	36.45675320806347	38.0	37.0	38.0	34.0	38.0
70-71	35.47369001776434	38.0	36.5	38.0	28.5	38.0
72-73	33.666317264512145	38.0	34.5	38.0	20.5	38.0
74-75	33.391629243856954	38.0	34.5	38.0	19.0	38.0
76	33.13091547042403	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	9.0
23	10.0
24	31.0
25	93.0
26	173.0
27	384.0
28	581.0
29	1063.0
30	1557.0
31	2612.0
32	4159.0
33	6566.0
34	11254.0
35	22637.0
36	30608.0
37	99525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.26999127813904	16.163595614780792	10.799651125561677	37.766761981518485
2	18.06721761869559	16.817645176595207	42.0523882556741	23.062748949035097
3	16.652690580485704	18.27465216096038	40.33774315631517	24.734914102238747
4	11.990378568039633	27.786849974070684	32.97988546965166	27.24288598823802
5	11.701845946751112	30.794650836910108	44.20176319360925	13.30174002272953
6	25.87083889618342	44.67235272699187	16.81047323763392	12.646335139190784
7	24.464587172159636	26.135648950138474	18.938332358685216	30.461431519016674
8	21.463958248281493	43.64731714314087	16.29850713332083	18.59021747525681
9	21.022056470744005	15.905705553287506	16.9820480850923	46.09018989087619
10-11	21.42561595921925	29.027595414372563	28.786507927750993	20.760280698657194
12-13	21.545332171111433	19.29555008771833	30.03911465171961	29.12000308945063
14-15	19.528362260153813	19.64394081495294	20.705663625028965	40.122033299864285
16-17	27.811400072822767	29.228133861482274	20.44416369674835	22.516302368946608
18-19	28.209442685173947	23.291699308183734	21.60987962176297	26.888978384879348
20-21	19.905716587039755	21.59829418190244	21.227560106365374	37.26842912469243
22-23	19.702695545674217	29.991669517052667	21.047985788527104	29.25764914874601
24-25	27.546018531544725	29.635575710932176	21.315965871945316	21.50243988557778
26-27	27.973872074676436	30.607352892498152	21.505334819211967	19.91344021361344
28-29	27.01090134722115	23.234323796493474	22.15329191998323	27.601482936302148
30-31	28.275093511050304	21.638015689995697	30.058699561960033	20.028191236993965
32-33	20.93902748507685	29.887124714501663	29.628659068089284	19.545188732332207
34-35	26.355220619876203	30.366265405876575	23.964758195319483	19.313755778927742
36-37	20.953095519193212	29.35750460659156	21.61925831117388	28.070141563041346
38-39	18.930332888305326	23.46575674989794	30.04352815261886	27.560382209177874
40-41	19.028257439507453	20.670355617834957	31.38826670785934	28.913120234798246
42-43	26.916711261661362	20.262770259460115	30.844748732490718	21.975769746387805
44-45	18.970606084010992	28.181030773135017	32.2014542485463	20.646908894307686
46-47	26.265295539053966	20.233419028809127	25.59334002714303	27.907945404993878
48-49	25.323013097063917	20.19204245787865	24.066268716002252	30.418675729055177
50-51	18.974036400354237	20.4312644667544	32.10619390899635	28.48850522389501
52-53	16.69311023241825	20.690825508337312	40.11401545775882	22.50204880148562
54-55	16.04823107642291	20.97924871460152	33.675370302396374	29.2971499065792
56-57	24.354735436008966	20.734298930262852	25.535420553525494	29.375545080202688
58-59	16.86737346365478	20.527559011661598	38.924792900387985	23.68027462429564
60-61	24.365490637572226	28.264984575310564	25.206260382002505	22.163264405114706
62-63	16.961770346150132	38.28677069105859	22.439192638273802	22.312266324517473
64-65	16.492211597353652	39.447997307303936	21.160563038332295	22.899228057010117
66-67	16.46647185269194	39.2809543125357	20.492719310590317	23.759854524182046
68-69	16.51507502338015	39.49410599552536	20.319349383564102	23.671469597530397
70-71	17.255953563309717	37.939482222075085	20.340605619192655	24.463958595422543
72-73	18.159746171885775	34.610842874879296	20.715409021934057	26.514001931300868
74-75	19.174223593059857	33.25341889420119	21.00774958548408	26.56460792725487
76	19.39746737661048	33.58015835793307	20.76806356388115	26.2543107015753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	7.5
16	23.0
17	31.0
18	44.5
19	74.0
20	115.5
21	141.0
22	153.0
23	207.0
24	343.0
25	437.0
26	530.5
27	674.0
28	902.5
29	1081.0
30	1188.0
31	1533.5
32	2122.5
33	2473.0
34	2777.0
35	3338.0
36	3876.5
37	4158.0
38	4658.0
39	5804.5
40	10508.0
41	16365.0
42	18165.0
43	17088.5
44	14651.0
45	11968.0
46	10646.0
47	10011.0
48	8877.5
49	8081.5
50	7784.0
51	7482.0
52	6645.0
53	5847.0
54	5584.0
55	5220.0
56	4321.0
57	3590.5
58	3395.0
59	3119.5
60	2615.5
61	2087.5
62	1788.0
63	1805.5
64	1701.0
65	1468.5
66	1242.0
67	1126.0
68	1122.5
69	1018.5
70	882.0
71	846.0
72	826.0
73	731.0
74	637.5
75	619.0
76	570.5
77	481.0
78	422.0
79	404.0
80	378.0
81	323.0
82	242.0
83	190.0
84	182.5
85	143.5
86	88.0
87	64.0
88	60.5
89	37.5
90	19.0
91	16.5
92	13.0
93	8.0
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05958226213988591
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0019309066434222287
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.51687612406351E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015171409341174653
52-53	0.03227372532577153
54-55	0.051031104147587464
56-57	0.05296201079100969
58-59	0.03806644525603822
60-61	0.03254956913197471
62-63	0.03006697487614613
64-65	0.01710231598459688
66-67	0.035032163387803295
68-69	0.010206220829517495
70-71	0.014343877922565128
72-73	0.02041244165903499
74-75	0.014619721728768303
76	0.014895565534971478
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	181262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.448113780053184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.33242216549756	10.247045712835565
2	18.449138745402287	10.127881188555792
3	15.128735955620767	12.457657975747813
4	10.867686370671114	11.931899681124559
5	6.877977207404578	9.439375048272666
6	4.104275119088296	6.759276627202612
7	2.2410708901975758	4.30592181483157
8	1.2541957268908408	2.7540245611325043
9	0.7657829678612345	1.8917368229413776
>10	2.9224368379796193	12.528825677748232
>50	0.026129077643559182	0.45514228023523956
>100	0.022109219544550078	1.2716399465966393
>500	0.0020099290495045525	0.331564255056217
>1k	0.004019858099009105	2.5162472001853673
>5k	0.0	0.0
>10k+	0.0020099290495045525	12.981761207533845
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	23531	12.981761207533845	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2880	1.5888603237302912	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1681	0.9273868764550761	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	601	0.331564255056217	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	484	0.2670168044046739	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	354	0.19529741479184826	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	348	0.19198728911741014	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	195	0.10757908441923844	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.51687612406351E-4	0.0
2	0.0	0.0	0.0	5.51687612406351E-4	0.0
3	0.0	0.0	0.0	5.51687612406351E-4	0.0
4	0.0	0.0	0.0	0.004965188511657159	0.0
5	0.0	0.0	0.0	0.007171938961282563	0.0
6	0.0	0.0	0.0	0.007171938961282563	0.0
7	0.0	0.0	0.0	0.009930377023314318	0.0
8	0.0	0.0	0.0	0.009930377023314318	0.0
9	0.0	0.0	0.0	0.012688815085346073	0.0
10	0.0	0.0	0.0	0.01599894075978418	0.0
11	0.0	0.0	0.0	0.01655062837219053	0.0
12	0.0	0.0	0.0	0.017654003597003233	0.0
13	0.0	0.0	0.0	0.017654003597003233	0.0
14	0.0	0.0	0.0	0.019860754046628636	0.0
15	0.0	0.0	0.0	0.02096412927144134	0.0
16	0.0	0.0	0.0	0.023170879721066743	0.0
17	0.0	0.0	0.0	0.024825942558285796	0.0
18	0.0	0.0	0.0	0.027584380620317553	0.0
19	0.0	0.0	0.0	0.029239443457536606	0.0
20	0.0	0.0	0.0	0.03254956913197471	0.0
21	0.0	0.0	0.0	0.03254956913197471	0.0
22	0.0	0.0	0.0	0.035859694806412816	0.0
23	0.0	0.0	0.0	0.04137657093047633	0.0
24	0.0	0.0	0.0	0.047445134666946186	0.0
25	0.0	0.0	0.0	0.04854850989175889	0.0
26	0.0	0.0	0.0	0.05461707362822875	0.0
27	0.0	0.0	0.0	0.08495989231057806	0.0
28	0.0	0.0	0.0	0.11750946144255277	0.0
29	0.0	0.0	0.0	0.14950734296212112	0.0
30	0.0	0.0	0.0	0.1914356015050038	0.0
31	0.0	0.0	0.0	0.23612229810991825	0.0
32	0.0	0.0	0.0	0.2719819929163311	0.0
33	0.0	0.0	0.0	0.33101256744381063	0.0
34	0.0	0.0	0.0	0.394456642870541	0.0
35	0.0	0.0	0.0	0.5301717955225034	0.0
36	0.0	0.0	0.0	0.738709713012104	0.0
37	0.0	0.0	0.0	1.0879279716653243	0.0
38	0.0	0.0	0.0	1.568447882071256	0.0
39	0.0	0.0	0.0	2.151581688384769	0.0
40	0.0	0.0	0.0	2.821330449846079	0.0
41	0.0	0.0	0.0	3.724994758967682	0.0
42	0.0	0.0	0.0	4.794717039423597	0.0
43	0.0	0.0	0.0	5.803753682514813	0.0
44	0.0	0.0	0.0	6.736657435093952	0.0
45	0.0	0.0	0.0	7.7556244552084825	0.0
46	0.0	0.0	0.0	8.792245478920016	0.0
47	0.0	0.0	0.0	9.746113360770597	0.0
48	0.0	0.0	0.0	10.51240745440302	0.0
49	0.0	0.0	0.0	11.32118149419073	0.0
50	0.0	0.0	0.0	12.053822643466363	0.0
51	0.0	0.0	0.0	12.855976431905198	0.0
52	0.0	0.0	0.0	13.840187132438128	0.0
53	0.0	0.0	0.0	14.775297635466893	0.0
54	0.0	0.0	0.0	15.723096953581004	0.0
55	0.0	0.0	0.0	16.53407774381834	0.0
56	0.0	0.0	0.0	17.27168408160563	0.0
57	0.0	0.0	0.0	18.00211848043164	0.0
58	0.0	0.0	0.0	18.622767044388784	0.0
59	0.0	0.0	0.0	19.230726793260583	0.0
60	0.0	0.0	0.0	19.79510322075228	0.0
61	0.0	0.0	0.0	20.393132592600764	0.0
62	0.0	0.0	0.0	21.015987907007535	0.0
63	0.0	0.0	0.0	21.722699738500072	0.0
64	0.0	0.0	0.0	22.399620438922664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	15	0.002210811	70.08812	1
CGTATGA	15	0.002222951	69.99145	41
GTATGAC	15	0.002222951	69.99145	42
AACTCGT	55	3.6379788E-11	57.26573	37
CGTCTTA	25	2.3838355E-4	56.00861	48
GCTTGAC	25	2.3838355E-4	56.00861	56
ATCTCGT	3365	0.0	53.76758	37
CGTCTTC	3425	0.0	53.657883	48
CTCGTAT	3485	0.0	53.623287	39
TCTCGTA	3380	0.0	53.528965	38
CATCTCG	3375	0.0	53.504574	36
CCGTCTT	3455	0.0	53.495922	47
TATGCCG	3470	0.0	53.451683	43
TCGTATG	3510	0.0	53.440765	40
GTCTTCT	3455	0.0	53.394604	49
TCTTCTG	3450	0.0	53.37052	50
CTGCTTG	3440	0.0	53.32215	54
CGTATGC	3505	0.0	53.31731	41
TGCCGTC	3475	0.0	53.28877	45
GCCGTCT	3470	0.0	53.26467	46
>>END_MODULE