FastQCFastQC Report
Wed 25 May 2016
SRR1779852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286568
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGC252.8323993E-670.0130936
CGGTGGT850.053.5300846
AAGTCGA200.006946395652.50065231
GTCATCG200.00695119252.4914934
AACGCGA200.00695119252.4914939
CCGTACC305.866564E-446.67539255
GACGAAT604.9258233E-946.65910723
GTAGATC1300.045.76980238
CGACGAG551.3096724E-744.53823522
CTCGGTG901.8189894E-1242.77831344
CGACGAA2700.042.77084722
TCGGTGG901.8189894E-1242.77084445
TGGTCGC801.4169927E-939.3754949
ACGAAGG459.1933856E-538.88258724
CTTCCGA556.6890625E-638.1756318
TCGCCGT904.476533E-935.00654652
CGTATCA806.21294E-835.00654656
ACTCGGT400.002416265435.00043543
TAGATCT1700.035.00043539
CTCTCGG501.7071608E-435.00043542