##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779852_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286568
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.001922754808632	33.0	23.0	34.0	12.0	34.0
2	29.236411602132826	34.0	27.0	34.0	12.0	34.0
3	28.68957106166774	34.0	23.0	34.0	11.0	34.0
4	28.858668797632674	34.0	24.0	34.0	12.0	34.0
5	29.83429063956897	34.0	31.0	34.0	11.0	34.0
6	32.086649591022024	37.0	31.0	38.0	12.0	38.0
7	32.344462745316996	37.0	31.0	38.0	12.0	38.0
8	31.34102551575891	37.0	27.0	38.0	11.0	38.0
9	32.53486083582256	37.0	31.0	38.0	12.0	38.0
10-11	30.87887866056224	36.5	26.0	38.0	11.0	38.0
12-13	31.868874054325673	37.0	29.5	38.0	11.0	38.0
14-15	30.60835124647553	36.0	25.5	38.0	11.0	38.0
16-17	31.323228692666312	36.5	28.0	38.0	11.0	38.0
18-19	32.388197565673764	37.0	31.0	38.0	11.0	38.0
20-21	30.803294506016023	36.5	26.0	38.0	11.0	38.0
22-23	31.860331579241226	37.0	29.0	38.0	11.0	38.0
24-25	32.159876538901756	37.0	31.0	38.0	11.0	38.0
26-27	30.976899025711177	37.0	26.5	38.0	11.0	38.0
28-29	32.389460791156026	37.0	32.0	38.0	11.0	38.0
30-31	33.02467128220876	37.0	34.0	38.0	11.0	38.0
32-33	33.12165524413054	37.0	34.0	38.0	16.0	38.0
34-35	32.96796746321989	37.0	33.0	38.0	16.0	38.0
36-37	32.33203114095084	37.0	31.5	38.0	11.0	38.0
38-39	32.261768585466626	37.0	31.0	38.0	11.0	38.0
40-41	32.1187623879847	37.0	29.5	38.0	11.0	38.0
42-43	32.65791714357499	37.0	32.5	38.0	16.0	38.0
44-45	32.703635786270624	37.0	32.0	38.0	11.0	38.0
46-47	32.575947070154385	37.0	31.5	38.0	11.0	38.0
48-49	32.17731742553251	37.0	29.5	38.0	11.0	38.0
50-51	31.41763560481282	36.5	28.5	38.0	11.0	38.0
52-53	32.03022842745875	37.0	30.0	38.0	11.0	38.0
54-55	32.28588502554368	37.0	31.5	38.0	11.0	38.0
56-57	32.380846779821894	37.0	31.0	38.0	11.0	38.0
58-59	32.774074565199186	37.0	32.5	38.0	11.0	38.0
60-61	32.51369657463499	37.0	31.5	38.0	11.0	38.0
62-63	32.579974037575724	37.0	32.0	38.0	11.0	38.0
64-65	32.5997529382206	37.0	31.5	38.0	11.0	38.0
66-67	32.37188381117222	37.0	31.0	38.0	11.0	38.0
68-69	31.518923955221798	37.0	27.5	38.0	11.0	38.0
70-71	31.519367131012537	37.0	27.5	38.0	11.0	38.0
72-73	31.83809776388152	37.0	30.5	38.0	11.0	38.0
74-75	31.972495184389047	37.0	29.5	38.0	11.0	38.0
76	30.494179391976772	35.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	30.0
13	440.0
14	1392.0
15	2537.0
16	3423.0
17	4323.0
18	4785.0
19	4888.0
20	4793.0
21	4663.0
22	4608.0
23	4923.0
24	5283.0
25	5806.0
26	6376.0
27	7038.0
28	8070.0
29	9094.0
30	10497.0
31	12128.0
32	14370.0
33	17590.0
34	22212.0
35	29557.0
36	44206.0
37	53533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.48374894439675	18.82986816300608	11.622802425968189	30.06358046662898
2	20.917897322799476	21.425281259596325	32.91504983110466	24.74177158649954
3	18.437857681248428	21.021537645515203	31.91284442087044	28.62776025236593
4	13.25339884425337	23.609754054884007	31.831188409033807	31.305658691828814
5	13.759735909103599	35.321459479076516	33.75638591887441	17.16241869294548
6	27.611729357942234	38.80577743812007	17.645334533767443	15.937158670170259
7	26.94326374784508	28.776426083739885	21.48670058697488	22.793609581440148
8	23.362855328266438	38.669625845355	18.130901781788488	19.836617044590078
9	23.81763359204869	18.460341169244515	19.784047127142777	37.93797811156402
10-11	23.419754572753142	27.11896050237035	24.551274333781752	24.910010591094757
12-13	23.94549277220899	23.0985718896944	25.76286564247516	27.193069695621453
14-15	25.364574427620767	25.02905079789369	22.154853838718346	27.451520935767203
16-17	25.711613688945345	25.86637679844504	23.002648595267424	25.419360917342193
18-19	22.426425043140828	25.61788656187241	25.79114779898905	26.164540595997714
20-21	24.698500879372435	25.175874487032747	25.04501549370481	25.080609139890008
22-23	25.26652079996371	25.11175553019755	24.224341593297112	25.397382076541636
24-25	22.48290061418202	27.691582914572866	24.113798157453935	25.711718313791177
26-27	24.194167507075136	27.69404711638116	24.268844633192238	23.842940743351466
28-29	22.789724583664867	26.984833256557366	26.12755978056033	24.097882379217445
30-31	23.187113636799836	26.420743527244113	25.525837807280332	24.86630502867572
32-33	22.157781325516677	27.022952707477295	25.574777321224495	25.244488645781537
34-35	22.354870792692502	27.41166617228804	25.718797438537106	24.514665596482352
36-37	24.096560052492705	25.93363023356462	25.424409108042834	24.545400605899843
38-39	23.990374883961167	25.85484850388425	26.18080421020304	23.973972401951546
40-41	22.64582206480205	26.7644338980685	25.605883481932544	24.9838605551969
42-43	21.61433115406828	26.198639398808993	26.631700716592597	25.555328730530125
44-45	21.27170635696139	24.633399871576536	28.07483458499679	26.020059186465282
46-47	21.38973610926933	24.68662539520795	27.737840158013388	26.185798337509336
48-49	21.562147769980495	25.467691674198356	27.68602107011624	25.284139485704916
50-51	23.443042568672233	25.529861931424602	26.392158025966594	24.634937473936567
52-53	19.674379646110353	26.299340383205948	27.45227375841971	26.574006212263985
54-55	20.25818069507771	25.940912985288445	26.772200381442957	27.028705938190882
56-57	20.119186807433906	25.952883692522466	26.774103481371608	27.153826018672017
58-59	19.974836884729548	25.745775755111584	27.47159551949967	26.807791840659196
60-61	19.787469088078687	25.419067048982637	27.55589451290491	27.23756935003377
62-63	19.95777130193519	26.423473572163957	26.44737990123371	27.171375224667145
64-65	20.932468869710906	25.61540676620215	25.754849518756707	27.697274845330238
66-67	20.27087397955444	26.838198082487253	24.945465714076303	27.945462223882007
68-69	20.191195381160675	27.928693440068674	24.346091905689974	27.534019273080673
70-71	20.80197790041753	27.39451401332675	24.912367635435405	26.891140450820316
72-73	20.4574046899074	27.256706037134688	24.577257491236303	27.70863178172161
74-75	20.170168510798685	26.719701821214876	24.64791067200159	28.462218995984845
76	19.39740158222216	25.92973872745229	24.97496152624765	29.697898164077902
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	2.5
7	4.5
8	5.0
9	2.5
10	1.0
11	1.5
12	1.0
13	1.5
14	5.0
15	13.0
16	23.5
17	29.0
18	51.0
19	88.0
20	128.0
21	153.0
22	215.0
23	346.0
24	488.5
25	562.0
26	775.5
27	1140.5
28	1498.5
29	1705.0
30	2030.5
31	2722.0
32	3689.5
33	4291.0
34	4663.0
35	5705.0
36	7087.0
37	7799.0
38	8860.5
39	10918.0
40	12818.0
41	14338.0
42	14954.0
43	15528.0
44	16760.5
45	17696.0
46	17973.0
47	17964.0
48	17417.0
49	16331.0
50	15783.0
51	15022.5
52	13220.5
53	11342.0
54	10505.0
55	9850.0
56	8515.0
57	7194.0
58	6553.0
59	5939.0
60	4936.5
61	4215.5
62	3883.0
63	3613.5
64	3115.0
65	2676.0
66	2364.0
67	2262.0
68	2110.5
69	1855.5
70	1646.0
71	1540.0
72	1473.5
73	1323.0
74	1164.0
75	1089.0
76	1023.5
77	894.0
78	786.5
79	743.0
80	672.5
81	548.0
82	439.5
83	385.0
84	352.0
85	254.5
86	177.5
87	165.0
88	147.5
89	108.5
90	83.5
91	63.5
92	47.0
93	38.0
94	26.5
95	16.5
96	9.0
97	10.0
98	12.0
99	14.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.97914631082326E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.001744786577705815
7	0.004885402417576282
8	6.97914631082326E-4
9	0.008374975572987912
10-11	0.0022682225510175596
12-13	1.744786577705815E-4
14-15	3.48957315541163E-4
16-17	3.48957315541163E-4
18-19	0.0022682225510175596
20-21	0.0
22-23	0.001744786577705815
24-25	0.002791658524329304
26-27	3.48957315541163E-4
28-29	0.00697914631082326
30-31	5.234359733117445E-4
32-33	1.744786577705815E-4
34-35	0.004536445102035119
36-37	0.018145780408140477
38-39	0.009072890204070238
40-41	0.001046871946623489
42-43	0.0012213506043940706
44-45	0.005583317048658608
46-47	0.00348957315541163
48-49	3.48957315541163E-4
50-51	0.0026171798665587226
52-53	0.013260377990564193
54-55	0.008549454230758494
56-57	0.01500516456827001
58-59	0.013085899332793612
60-61	0.023903576114569666
62-63	0.011515591412858378
64-65	0.024601490745651995
66-67	0.017796823092599313
68-69	8.723932888529075E-4
70-71	5.234359733117445E-4
72-73	0.005757795706429189
74-75	0.009945283492923146
76	0.0031406158398704673
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	286568.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.50154886394564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.47651665277385	58.852237177875075
2	8.164983549639103	11.512879733963873
3	3.7706679220618975	7.975137862708777
4	1.7983814581428146	5.071547129890777
5	0.8830443037011066	3.112799556320621
6	0.49620099087210556	2.0989763042584797
7	0.3155902723506857	1.5574722104982448
8	0.20313028990302157	1.1456800047476254
9	0.1667572366798761	1.058097912318255
>10	0.7237153205625274	7.57878472889831
>50	0.0010120033129663725	0.03638737851998253
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001046871946623489	0.0
2	0.0	0.0	0.0	0.001395829262164652	0.0
3	0.0	0.0	0.0	0.001744786577705815	0.0
4	0.0	0.0	0.0	0.002791658524329304	0.0
5	0.0	0.0	0.0	0.0059322743641997714	0.0
6	0.0	0.0	0.0	0.0059322743641997714	0.0
7	0.0	0.0	0.0	0.0062812316797409345	0.0
8	0.0	0.0	0.0	0.006630188995282097	0.0
9	0.0	0.0	0.0	0.006630188995282097	0.0
10	0.0	0.0	0.0	0.006630188995282097	0.0
11	0.0	0.0	0.0	0.00697914631082326	0.0
12	0.0	0.0	0.0	0.007677060941905586	0.0
13	0.0	0.0	0.0	0.008374975572987912	0.0
14	0.0	0.0	0.0	0.008723932888529075	0.0
15	0.0	0.0	0.0	0.008723932888529075	0.0
16	0.0	0.0	0.0	0.008723932888529075	0.0
17	0.0	0.0	0.0	0.008723932888529075	0.0
18	0.0	0.0	0.0	0.009770804835152564	0.0
19	0.0	0.0	0.0	0.012562463359481869	0.0
20	0.0	0.0	0.0	0.013609335306105357	0.0
21	0.0	0.0	0.0	0.014656207252728846	0.0
22	0.0	0.0	0.0	0.01849473772368164	0.0
23	0.0	0.0	0.0	0.020239524301387455	0.0
24	0.0	0.0	0.0	0.02303118282571676	0.0
25	0.0	0.0	0.0	0.027567627927751876	0.0
26	0.0	0.0	0.0	0.032104073029786996	0.0
27	0.0	0.0	0.0	0.06944250579269144	0.0
28	0.0	0.0	0.0	0.0952653471427375	0.0
29	0.0	0.0	0.0	0.1074788531866782	0.0
30	0.0	0.0	0.0	0.12667150554144216	0.0
31	0.0	0.0	0.0	0.15528600541581752	0.0
32	0.0	0.0	0.0	0.1828536333435694	0.0
33	3.48957315541163E-4	0.0	0.0	0.2153066636888976	0.0
34	3.48957315541163E-4	0.0	0.0	0.24845760866530806	0.0
35	3.48957315541163E-4	0.0	0.0	0.31545741324921134	0.0
36	3.48957315541163E-4	0.0	0.0	0.45399346751905306	0.0
37	3.48957315541163E-4	0.0	0.0	0.6965188018201613	0.0
38	3.48957315541163E-4	0.0	0.0	1.066064598978253	0.0
39	3.48957315541163E-4	0.0	0.0	1.448870774126909	0.0
40	3.48957315541163E-4	0.0	0.0	1.9775411071717708	0.0
41	3.48957315541163E-4	0.0	0.0	2.7204712319589066	0.0
42	3.48957315541163E-4	0.0	0.0	3.657072666871388	0.0
43	3.48957315541163E-4	0.0	0.0	4.623335473604869	0.0
44	3.48957315541163E-4	0.0	0.0	5.317062616900701	0.0
45	3.48957315541163E-4	0.0	0.0	5.950420144607912	0.0
46	3.48957315541163E-4	0.0	0.0	6.58901203204824	0.0
47	3.48957315541163E-4	0.0	0.0	7.160604114904665	0.0
48	3.48957315541163E-4	0.0	0.0	7.697649423522515	0.0
49	3.48957315541163E-4	0.0	0.0	8.186189665280143	0.0
50	3.48957315541163E-4	0.0	0.0	8.741380754306133	0.0
51	3.48957315541163E-4	0.0	0.0	9.40649339772759	0.0
52	3.48957315541163E-4	0.0	0.0	10.308897015717038	0.0
53	3.48957315541163E-4	0.0	0.0	11.404622986516289	0.0
54	3.48957315541163E-4	0.0	0.0	12.257823623014433	0.0
55	3.48957315541163E-4	0.0	0.0	12.924332095698054	0.0
56	3.48957315541163E-4	0.0	0.0	13.536054269841713	0.0
57	3.48957315541163E-4	0.0	0.0	14.109391139275845	0.0
58	3.48957315541163E-4	0.0	0.0	14.63910834426733	0.0
59	3.48957315541163E-4	0.0	0.0	15.086122665475559	0.0
60	3.48957315541163E-4	0.0	0.0	15.553376510985176	0.0
61	3.48957315541163E-4	0.0	0.0	15.99550542977583	0.0
62	3.48957315541163E-4	0.0	0.0	16.576170402836325	0.0
63	3.48957315541163E-4	0.0	0.0	17.178470729460372	0.0
64	3.48957315541163E-4	0.0	0.0	17.742036794059352	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCGC	25	2.8323993E-6	70.01309	36
CGGTGGT	85	0.0	53.53008	46
AAGTCGA	20	0.0069463956	52.500652	31
GTCATCG	20	0.006951192	52.491493	4
AACGCGA	20	0.006951192	52.491493	9
CCGTACC	30	5.866564E-4	46.675392	55
GACGAAT	60	4.9258233E-9	46.659107	23
GTAGATC	130	0.0	45.769802	38
CGACGAG	55	1.3096724E-7	44.538235	22
CTCGGTG	90	1.8189894E-12	42.778313	44
CGACGAA	270	0.0	42.770847	22
TCGGTGG	90	1.8189894E-12	42.770844	45
TGGTCGC	80	1.4169927E-9	39.37549	49
ACGAAGG	45	9.1933856E-5	38.882587	24
CTTCCGA	55	6.6890625E-6	38.17563	18
TCGCCGT	90	4.476533E-9	35.006546	52
CGTATCA	80	6.21294E-8	35.006546	56
ACTCGGT	40	0.0024162654	35.000435	43
TAGATCT	170	0.0	35.000435	39
CTCTCGG	50	1.7071608E-4	35.000435	42
>>END_MODULE