FastQCFastQC Report
Wed 25 May 2016
SRR1779852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286568
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT176596.162237235141397No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG24760.8640183132799196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC14550.5077328941123922No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5250.18320259065911057TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC4700.16400993830434663No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA252.3842335E-456.01787664
CGACATA252.3904115E-455.98855618
TTAGCCG200.006937973752.51675869
GACGTGC200.006937973752.51675854
AAACGAC200.00694276652.5075970
ACGGCAC200.006952357552.4892710
ACGGTCT200.006952357552.489278
ATCTCGT24950.051.3321937
TCTCGTA24950.051.3321938
AATCTCG24900.051.154236
CTCGTAT26050.050.91089639
TATGCCG26000.050.87421443
TCGTATG26150.050.85002540
CGTCTTC25700.050.8138848
CGTATGC26200.050.7529841
GTATGCC26150.050.58239442
CCGTCTT25750.050.5792547
GCCGTCT26000.050.3622346
ATGCCGT26400.049.97084444
GTCTTCT26300.049.6546349
Produced by FastQC (version 0.11.5)