FastQCFastQC Report
Wed 25 May 2016
SRR1779851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521488
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG2350.047.65337445
TGGTCGC1350.044.08101749
GACGAAT953.6379788E-1240.5210423
AGATCTC3350.039.70013440
CTCGGTG2350.038.72207644
GTAGATC3450.036.5204938
TCTCGGT2600.036.3449143
TAGATCT3750.035.4620539
CGACGAC2150.034.181622
GACGACA1450.033.78870823
ACGAATA1154.0017767E-1133.47390424
GATCTCG3050.033.2775541
TCGCTTA652.0995652E-532.31278249
ACGATAT652.1037013E-532.30348624
TCGACGA552.9935135E-431.81404121
ATCTCGG2900.031.37823542
CGGTGGT1900.031.3177246
ACGAAGG1251.05501385E-1030.79599424
GTGGTCG2050.030.73360348
CGTGTAG703.4857556E-530.0018535
Produced by FastQC (version 0.11.5)