##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779851_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 521488 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.410274061915135 34.0 27.0 34.0 12.0 34.0 2 30.458921394164392 34.0 32.0 34.0 21.0 34.0 3 29.8856733040837 34.0 31.0 34.0 11.0 34.0 4 30.324064983278618 34.0 31.0 34.0 12.0 34.0 5 31.026067713926302 34.0 32.0 34.0 21.0 34.0 6 33.37520901727365 37.0 34.0 38.0 21.0 38.0 7 33.713872610683275 38.0 34.0 38.0 21.0 38.0 8 33.002527383180436 37.0 34.0 38.0 11.0 38.0 9 33.978998557972574 38.0 34.0 38.0 21.0 38.0 10-11 32.45445053385696 37.0 30.5 38.0 16.5 38.0 12-13 33.362016959163014 37.0 34.0 38.0 21.0 38.0 14-15 32.133187149081095 37.0 29.5 38.0 11.0 38.0 16-17 32.81308390605345 37.0 32.5 38.0 16.0 38.0 18-19 33.76654016966833 37.5 34.0 38.0 21.0 38.0 20-21 32.31258629153499 37.0 30.5 38.0 16.0 38.0 22-23 33.30563311140429 37.5 33.0 38.0 16.0 38.0 24-25 33.61690297763323 38.0 34.0 38.0 21.0 38.0 26-27 32.49320406222195 37.0 31.5 38.0 11.0 38.0 28-29 33.82182044457399 37.5 34.5 38.0 21.5 38.0 30-31 34.28367862731261 38.0 36.0 38.0 23.0 38.0 32-33 34.362019835547514 38.0 36.0 38.0 23.0 38.0 34-35 34.24982741693002 38.0 35.5 38.0 22.5 38.0 36-37 33.73124117908753 38.0 34.0 38.0 21.5 38.0 38-39 33.66049937870095 38.0 34.0 38.0 21.0 38.0 40-41 33.62666542662535 38.0 34.0 38.0 21.0 38.0 42-43 34.05140290860001 38.0 35.0 38.0 22.5 38.0 44-45 34.081651926794095 38.0 35.0 38.0 22.0 38.0 46-47 33.98147512502685 38.0 34.5 38.0 21.5 38.0 48-49 33.684130794955976 38.0 34.0 38.0 21.5 38.0 50-51 33.010308003252234 37.5 32.5 38.0 16.0 38.0 52-53 33.58817652563434 37.0 34.0 38.0 21.0 38.0 54-55 33.757284923143004 37.5 34.0 38.0 21.5 38.0 56-57 33.829090985794494 38.0 34.0 38.0 21.5 38.0 58-59 34.13451603871997 38.0 35.0 38.0 22.5 38.0 60-61 33.94104754088301 38.0 34.0 38.0 22.0 38.0 62-63 33.98407250023011 38.0 34.5 38.0 22.0 38.0 64-65 33.999696062037856 38.0 34.5 38.0 22.0 38.0 66-67 33.80398110790661 38.0 34.0 38.0 21.0 38.0 68-69 33.120012349277445 37.0 33.5 38.0 15.5 38.0 70-71 33.12271231552788 37.0 33.5 38.0 19.5 38.0 72-73 33.382341492038165 37.0 34.0 38.0 21.0 38.0 74-75 33.47172801675207 37.5 33.5 38.0 21.0 38.0 76 32.20701722762556 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 33.0 13 517.0 14 2066.0 15 4372.0 16 6053.0 17 7236.0 18 7453.0 19 6970.0 20 6069.0 21 5214.0 22 4949.0 23 4894.0 24 5142.0 25 5902.0 26 6746.0 27 7980.0 28 9115.0 29 11331.0 30 13364.0 31 16446.0 32 20374.0 33 26208.0 34 35699.0 35 51658.0 36 89013.0 37 166683.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.14067491744745 18.101157078042363 10.128363944573776 31.62980405993641 2 20.855321694842452 22.407994047801676 30.473951461970362 26.262732795385514 3 19.02095541987543 20.716104685055072 29.55235786825392 30.710582026815576 4 14.342420151566287 19.34982204767895 32.355106924799806 33.95265087595496 5 15.039655754303071 32.48320191452152 34.06118645107845 18.415955880096956 6 29.71956093183091 35.62193653301833 17.91538502841977 16.743117506730997 7 29.126450051491332 27.929971790038106 20.019292468841513 22.924285689629052 8 25.007574740637047 34.559993863501255 19.452125721518016 20.980305674343683 9 24.69795182571341 16.66404571954587 19.980055231666157 38.657947223074565 10-11 24.845700763513946 24.784623483015817 24.377313769427033 25.9923619840432 12-13 24.953594330070874 22.051610008283987 25.260312797226398 27.73448286441874 14-15 24.78760154903933 23.83273136593705 22.41773245417649 28.961934630847132 16-17 25.623219031766915 25.368945666806013 22.903011777880902 26.104823523546173 18-19 23.470190039695478 25.085910982415093 25.066638541047425 26.377260436842004 20-21 24.4540622219495 24.533738072592275 25.060595833461175 25.951603871997055 22-23 25.728848856987803 24.625819553457227 23.745812343112682 25.89951924644229 24-25 23.773343931608526 26.49747779587837 23.473134043571182 26.25604422894192 26-27 24.30448222565688 26.336095972631846 24.459233492239427 24.90018830947185 28-29 23.699766710230712 25.88797296791954 25.14169500886463 25.27056531298512 30-31 23.84452462153717 25.676793845262896 25.14408386059413 25.334597672605803 32-33 22.91931933237198 26.179701162826376 25.000287638449976 25.90069186635167 34-35 23.07062424251722 26.53471764110274 25.113430649095626 25.281227467284417 36-37 24.5215093051312 25.146966026633216 24.93656246223956 25.394962205996023 38-39 24.4250280713833 24.912237972455046 25.67512755579038 24.98760640037128 40-41 23.249530424377472 25.511451508826294 25.564090001275215 25.67492806552102 42-43 22.06987935403641 24.876194786230464 26.42101538971916 26.63291047001397 44-45 22.023679396163892 23.777197362819436 27.719448631358517 26.479674609658154 46-47 21.908441960026657 24.148349145928115 27.437688468711197 26.505520425334026 48-49 21.66105929875462 24.87801697647015 27.526982961208006 25.933940763567225 50-51 22.842026336934033 25.097847827212533 26.62937486696339 25.43075096889005 52-53 19.988531296327906 26.09762973731299 26.9788787903778 26.934960175981317 54-55 20.595773814603614 25.351838516261232 26.791794637728827 27.260593031406327 56-57 20.936423291098453 25.647403816002583 26.286249099795846 27.129923793103117 58-59 20.479727973871377 25.36695431038864 26.994143881953242 27.159173833786742 60-61 20.545510866198217 24.78649773814673 27.187600996999223 27.480390398655835 62-63 21.2047173800686 25.234282032128736 26.05768520818091 27.503315379621757 64-65 21.55681272195279 24.636028590252728 25.4703744870451 28.336784200749378 66-67 21.0116429272166 26.040321103512827 24.847163205242914 28.100872764027663 68-69 21.192056154965 26.622696124820344 24.190989011199736 27.994258709014918 70-71 21.391465926218537 26.682403746217542 24.761642690311916 27.164487637252005 72-73 21.117459745195934 26.40780348234139 24.3762938444884 28.098442927974272 74-75 20.695206753579498 25.82521162468693 24.640420219314898 28.83916140241868 76 19.91497146539028 25.42188267059401 25.074788291605305 29.588357572410406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 2.0 8 1.0 9 2.0 10 3.0 11 5.0 12 7.0 13 18.5 14 36.5 15 55.5 16 107.0 17 146.0 18 180.0 19 301.0 20 468.5 21 549.0 22 682.5 23 1037.5 24 1468.0 25 1677.0 26 1982.0 27 2760.0 28 3642.0 29 4051.0 30 4652.5 31 6014.5 32 7658.5 33 8542.0 34 9272.0 35 11141.0 36 13129.5 37 13979.0 38 15013.0 39 17479.5 40 20195.5 41 22221.5 42 22964.0 43 23665.0 44 25089.5 45 26187.5 46 26562.0 47 26681.0 48 26345.0 49 24974.5 50 24059.0 51 23551.5 52 21993.0 53 20164.5 54 19387.0 55 18694.0 56 17157.5 57 15487.5 58 14661.0 59 13747.0 60 12226.5 61 10872.5 62 10125.0 63 9507.0 64 8386.5 65 7470.5 66 6644.0 67 6231.0 68 5949.5 69 5372.5 70 4711.5 71 4346.0 72 4213.0 73 3824.5 74 3398.5 75 3228.0 76 2973.0 77 2579.0 78 2233.0 79 2026.0 80 1890.5 81 1541.0 82 1181.0 83 1035.0 84 894.5 85 650.0 86 503.5 87 461.0 88 385.5 89 252.5 90 160.5 91 110.0 92 94.0 93 81.0 94 63.5 95 47.0 96 35.0 97 30.5 98 25.0 99 27.5 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.8351793329856104E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0038351793329856106 7 0.007862117632620501 8 0.00345166139968705 9 0.009204430399165464 10-11 0.003355781916362409 12-13 0.0 14-15 6.711563832724818E-4 16-17 3.8351793329856104E-4 18-19 0.00345166139968705 20-21 0.0 22-23 0.0028763844997392075 24-25 0.00373929984966097 26-27 0.001150553799895683 28-29 0.006423925382750897 30-31 6.711563832724818E-4 32-33 0.0 34-35 0.004602215199582732 36-37 0.020901727364771576 38-39 0.009492068849139385 40-41 0.0012464332832203234 42-43 6.711563832724818E-4 44-45 0.004218697266284171 46-47 0.002972263983063848 48-49 2.8763844997392075E-4 50-51 0.0028763844997392075 52-53 0.013231368698800356 54-55 0.009300309882490105 56-57 0.01505307888196852 58-59 0.012943730248826436 60-61 0.023682232381186143 62-63 0.010450863682385788 64-65 0.023298714447887583 66-67 0.01888825821495413 68-69 4.7939741662320133E-4 70-71 3.8351793329856104E-4 72-73 0.005465130549504494 74-75 0.008820912465866904 76 0.0030681434663884883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 521488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.62107083025302 #Duplication Level Percentage of deduplicated Percentage of total 1 75.64640994014731 45.85766375036027 2 9.196551726066739 11.15009627159955 3 6.163116165284542 11.208441047723747 4 3.789283255048782 9.188416344008157 5 2.2066254411793187 6.688399858278489 6 1.2416971335800382 4.516380593068659 7 0.592600877095663 2.514686982114038 8 0.33549470530911185 1.6270438634974829 9 0.17898092251376044 0.9765013662873631 >10 0.6447769561418782 6.120478558167547 >50 0.004462877632822167 0.15189136489468258 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00364342036633633 0.0 2 0.0 0.0 0.0 0.0040269382996348906 0.0 3 0.0 0.0 0.0 0.0040269382996348906 0.0 4 0.0 0.0 0.0 0.0074785996993219405 0.0 5 0.0 0.0 0.0 0.009587948332464026 0.0 6 0.0 0.0 0.0 0.009587948332464026 0.0 7 0.0 0.0 0.0 0.009779707299113307 0.0 8 1.9175896664928052E-4 0.0 0.0 0.009779707299113307 0.0 9 1.9175896664928052E-4 0.0 0.0 0.011889055932255392 1.9175896664928052E-4 10 1.9175896664928052E-4 0.0 0.0 0.012847850765501795 1.9175896664928052E-4 11 1.9175896664928052E-4 0.0 0.0 0.013806645598748198 1.9175896664928052E-4 12 1.9175896664928052E-4 0.0 0.0 0.01438192249869604 1.9175896664928052E-4 13 1.9175896664928052E-4 0.0 0.0 0.014957199398643881 1.9175896664928052E-4 14 1.9175896664928052E-4 0.0 0.0 0.016107753198539562 1.9175896664928052E-4 15 1.9175896664928052E-4 0.0 0.0 0.016491271131838126 1.9175896664928052E-4 16 1.9175896664928052E-4 0.0 0.0 0.016491271131838126 1.9175896664928052E-4 17 1.9175896664928052E-4 0.0 0.0 0.016491271131838126 1.9175896664928052E-4 18 1.9175896664928052E-4 0.0 0.0 0.017066548031785967 1.9175896664928052E-4 19 1.9175896664928052E-4 0.0 0.0 0.01821710183168165 1.9175896664928052E-4 20 1.9175896664928052E-4 0.0 0.0 0.018408860798330928 1.9175896664928052E-4 21 1.9175896664928052E-4 0.0 0.0 0.021477004264719417 1.9175896664928052E-4 22 1.9175896664928052E-4 0.0 0.0 0.027996809130794956 1.9175896664928052E-4 23 1.9175896664928052E-4 0.0 0.0 0.03125671156383272 1.9175896664928052E-4 24 1.9175896664928052E-4 0.0 0.0 0.036817721596661857 1.9175896664928052E-4 25 1.9175896664928052E-4 0.0 0.0 0.0383517933298561 1.9175896664928052E-4 26 1.9175896664928052E-4 0.0 0.0 0.04909029546221581 1.9175896664928052E-4 27 1.9175896664928052E-4 0.0 0.0 0.12272573865553953 1.9175896664928052E-4 28 1.9175896664928052E-4 0.0 0.0 0.16567974718497838 1.9175896664928052E-4 29 1.9175896664928052E-4 0.0 0.0 0.1890743411161906 1.9175896664928052E-4 30 1.9175896664928052E-4 0.0 0.0 0.22282391924646397 1.9175896664928052E-4 31 1.9175896664928052E-4 0.0 0.0 0.26136747154296935 1.9175896664928052E-4 32 1.9175896664928052E-4 0.0 0.0 0.30163685453931827 1.9175896664928052E-4 33 1.9175896664928052E-4 0.0 0.0 0.3507271500015341 1.9175896664928052E-4 34 1.9175896664928052E-4 0.0 0.0 0.4182263062620808 1.9175896664928052E-4 35 1.9175896664928052E-4 0.0 0.0 0.5313640965851564 1.9175896664928052E-4 36 1.9175896664928052E-4 0.0 0.0 0.7564891234314116 1.9175896664928052E-4 37 1.9175896664928052E-4 0.0 0.0 1.1467186205626976 1.9175896664928052E-4 38 1.9175896664928052E-4 0.0 0.0 1.6483600773172153 1.9175896664928052E-4 39 1.9175896664928052E-4 0.0 0.0 2.1787653790691253 1.9175896664928052E-4 40 1.9175896664928052E-4 0.0 0.0 2.797379805479704 1.9175896664928052E-4 41 1.9175896664928052E-4 0.0 0.0 3.687141410732366 1.9175896664928052E-4 42 1.9175896664928052E-4 0.0 0.0 4.855337035559783 1.9175896664928052E-4 43 1.9175896664928052E-4 0.0 0.0 5.965237934525819 1.9175896664928052E-4 44 1.9175896664928052E-4 0.0 0.0 6.809936182615899 1.9175896664928052E-4 45 1.9175896664928052E-4 0.0 0.0 7.5781226030129165 1.9175896664928052E-4 46 1.9175896664928052E-4 0.0 0.0 8.332694136777835 1.9175896664928052E-4 47 1.9175896664928052E-4 0.0 0.0 9.062528763844997 1.9175896664928052E-4 48 1.9175896664928052E-4 0.0 0.0 9.71776915288559 1.9175896664928052E-4 49 1.9175896664928052E-4 0.0 0.0 10.315673610898045 1.9175896664928052E-4 50 1.9175896664928052E-4 0.0 0.0 10.913194550977204 1.9175896664928052E-4 51 1.9175896664928052E-4 0.0 0.0 11.63631761421164 1.9175896664928052E-4 52 1.9175896664928052E-4 0.0 0.0 12.606617985457 1.9175896664928052E-4 53 1.9175896664928052E-4 0.0 0.0 13.628885036664315 1.9175896664928052E-4 54 1.9175896664928052E-4 0.0 0.0 14.462077746755439 1.9175896664928052E-4 55 1.9175896664928052E-4 0.0 0.0 15.184625533089926 1.9175896664928052E-4 56 1.9175896664928052E-4 0.0 0.0 15.862685239161783 1.9175896664928052E-4 57 1.9175896664928052E-4 0.0 0.0 16.50622833123677 1.9175896664928052E-4 58 1.9175896664928052E-4 0.0 0.0 17.06098702175314 1.9175896664928052E-4 59 1.9175896664928052E-4 0.0 0.0 17.604623692203848 1.9175896664928052E-4 60 1.9175896664928052E-4 0.0 0.0 18.146342772988064 1.9175896664928052E-4 61 1.9175896664928052E-4 0.0 0.0 18.71759273463627 1.9175896664928052E-4 62 1.9175896664928052E-4 0.0 0.0 19.293253152517412 1.9175896664928052E-4 63 1.9175896664928052E-4 0.0 0.0 19.89882796919584 1.9175896664928052E-4 64 1.9175896664928052E-4 0.0 0.0 20.487144478875834 1.9175896664928052E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGTGG 235 0.0 47.653374 45 TGGTCGC 135 0.0 44.081017 49 GACGAAT 95 3.6379788E-12 40.52104 23 AGATCTC 335 0.0 39.700134 40 CTCGGTG 235 0.0 38.722076 44 GTAGATC 345 0.0 36.52049 38 TCTCGGT 260 0.0 36.34491 43 TAGATCT 375 0.0 35.46205 39 CGACGAC 215 0.0 34.1816 22 GACGACA 145 0.0 33.788708 23 ACGAATA 115 4.0017767E-11 33.473904 24 GATCTCG 305 0.0 33.27755 41 TCGCTTA 65 2.0995652E-5 32.312782 49 ACGATAT 65 2.1037013E-5 32.303486 24 TCGACGA 55 2.9935135E-4 31.814041 21 ATCTCGG 290 0.0 31.378235 42 CGGTGGT 190 0.0 31.31772 46 ACGAAGG 125 1.05501385E-10 30.795994 24 GTGGTCG 205 0.0 30.733603 48 CGTGTAG 70 3.4857556E-5 30.00185 35 >>END_MODULE