FastQCFastQC Report
Wed 25 May 2016
SRR1779851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521488
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT306345.874344184334059TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG37880.7263829656674746TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC21990.4216779676617679RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT8050.15436596815267084TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6680.1280949897217194RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG407.464387E-752.5122348
GACGATC200.00694615252.50719569
TCTCGTA43250.052.3454738
CTCGTAT45200.052.33222639
CGTATGC45850.052.12454641
TCGTATG46000.052.03064340
TATGCCG45800.051.8758543
ATCTCGT43600.051.84501337
CCGTCTT45750.051.72789447
GCCGTCT45800.051.67142546
ATGCCGT45800.051.64665244
TGCCGTC46100.051.40124545
GTATGCC46350.051.33577342
CGTCTTC45950.051.27418548
GATCTCG44200.050.90373636
CTGCTTG45850.050.86617754
CTTGAAA45800.050.3913357
TGCTTGA46350.050.2419155
GCTTGAA46350.050.02005456
GTCTTCT47100.049.95273249
Produced by FastQC (version 0.11.5)