##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779851_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521488
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.561052219801795	34.0	34.0	34.0	34.0	34.0
2	33.639464378854356	34.0	34.0	34.0	34.0	34.0
3	33.76794863927837	34.0	34.0	34.0	34.0	34.0
4	33.58415150492437	34.0	34.0	34.0	34.0	34.0
5	33.614583652931614	34.0	34.0	34.0	34.0	34.0
6	37.29214670327985	38.0	38.0	38.0	37.0	38.0
7	37.36755399932501	38.0	38.0	38.0	37.0	38.0
8	37.25734053324334	38.0	38.0	38.0	36.0	38.0
9	37.17154373638511	38.0	38.0	38.0	36.0	38.0
10-11	37.125090126714326	38.0	38.0	38.0	35.5	38.0
12-13	37.29170757524622	38.0	38.0	38.0	36.5	38.0
14-15	37.31228810634185	38.0	38.0	38.0	36.5	38.0
16-17	37.248995183014756	38.0	38.0	38.0	36.0	38.0
18-19	37.13351122173473	38.0	38.0	38.0	36.0	38.0
20-21	37.127859126192746	38.0	38.0	38.0	36.0	38.0
22-23	37.08834335593532	38.0	38.0	38.0	36.0	38.0
24-25	37.28306212223484	38.0	38.0	38.0	36.0	38.0
26-27	37.21386014635044	38.0	38.0	38.0	36.0	38.0
28-29	36.75865408216488	38.0	37.0	38.0	35.0	38.0
30-31	37.001851432823	38.0	38.0	38.0	36.0	38.0
32-33	37.05186313011997	38.0	38.0	38.0	36.0	38.0
34-35	37.13158596170957	38.0	38.0	38.0	36.0	38.0
36-37	37.14418548461326	38.0	38.0	38.0	36.0	38.0
38-39	36.75457441014942	38.0	38.0	38.0	34.5	38.0
40-41	36.92724568925843	38.0	37.5	38.0	35.5	38.0
42-43	37.19316839505415	38.0	38.0	38.0	36.0	38.0
44-45	36.962098840241765	38.0	38.0	38.0	35.5	38.0
46-47	37.103798169852425	38.0	38.0	38.0	36.0	38.0
48-49	36.77747234835701	38.0	37.0	38.0	35.0	38.0
50-51	36.9517870018102	38.0	37.5	38.0	35.5	38.0
52-53	36.882388472985	38.0	38.0	38.0	35.5	38.0
54-55	36.88147665909858	38.0	37.5	38.0	35.5	38.0
56-57	36.76753060473108	38.0	37.0	38.0	35.0	38.0
58-59	36.86203134108551	38.0	37.5	38.0	35.5	38.0
60-61	36.56245110146351	38.0	37.0	38.0	34.0	38.0
62-63	36.36554244776485	38.0	37.0	38.0	34.0	38.0
64-65	36.70140827785107	38.0	37.0	38.0	34.5	38.0
66-67	36.49818691897033	38.0	37.0	38.0	34.0	38.0
68-69	36.622653829043045	38.0	37.0	38.0	34.0	38.0
70-71	36.16239971006044	38.0	37.0	38.0	32.5	38.0
72-73	35.37476988924002	38.0	37.0	38.0	26.5	38.0
74-75	35.25911813886417	38.0	37.0	38.0	26.0	38.0
76	34.95703640352223	38.0	37.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	7.0
22	28.0
23	66.0
24	140.0
25	267.0
26	511.0
27	924.0
28	1505.0
29	2564.0
30	4028.0
31	6344.0
32	10093.0
33	15995.0
34	26367.0
35	48223.0
36	78107.0
37	326317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.257213627755895	17.07325964455172	10.86912726568061	31.800399462011775
2	20.389922682784647	19.036296137207376	35.73389991716013	24.83988126284785
3	19.47408185806768	19.602560365722702	33.261743319117606	27.661614457092014
4	14.12017150921977	21.709799650231645	32.910632651182766	31.259396189365813
5	14.317107967968582	31.628532200165683	37.928389531494496	16.125970300371247
6	29.590901420550423	38.69063142392538	17.215544748872457	14.502922406651736
7	27.967661767864264	27.494975915073788	19.505913846531463	25.03144847053048
8	24.72520940079158	38.02196790721934	17.879606050378914	19.37321664161016
9	23.756634860246066	16.480916147639064	18.821142576626883	40.941306415487986
10-11	24.70612938360998	26.35477709937717	26.503294419047034	22.43579909796582
12-13	24.837867793698035	20.710256036572268	27.023344736599885	27.428531433129816
14-15	22.404254747952017	21.469525665020093	21.447952781272054	34.67826680575584
16-17	26.523524990028534	27.17636839198601	22.191498174454637	24.10860844353082
18-19	26.327259687662995	24.256262847850767	23.39986730279508	26.016610161691162
20-21	22.903786856073392	23.4941168349032	23.087875464056697	30.51422084496671
22-23	22.94424799803639	27.016537293283832	22.990270150032217	27.048944558647563
24-25	26.311893671653124	27.302058184509825	22.798233485912185	23.587814657924863
26-27	26.25669238793606	27.601977418464084	23.13706163900224	23.004268554597612
28-29	22.474246770779	27.548860184702235	23.479063756021233	26.49782928849753
30-31	22.231767557450986	27.77724511398153	23.34262725125027	26.648360077317218
32-33	22.03224235265241	31.532652716841042	23.485104163470684	22.950000767035867
34-35	26.20635565919062	24.34869066977572	27.077708403644955	22.367245267388704
36-37	26.432535360353448	26.616719847820086	24.273425275365877	22.67731951646059
38-39	22.581440033135948	24.068434940017795	27.299190010124875	26.050935016721382
40-41	21.802994508023197	22.77319899978523	28.293843770134693	27.129962722056884
42-43	25.435006884173305	22.15361486214395	28.32885254829468	24.082525705388065
44-45	21.74479566164514	25.51966679961955	29.654948915411282	23.080588623324026
46-47	24.516096247660542	22.205975976436658	26.670124720031907	26.607803055870892
48-49	24.061052219801798	22.051897646733963	26.29715352989906	27.589896603565183
50-51	20.716809788888728	22.88181508704804	29.72070789162028	26.680667232442957
52-53	19.427692354909002	23.160946659300862	33.25833256606205	24.153028419728077
54-55	19.288237178810768	23.284864368116004	29.900312148066003	27.52658630500722
56-57	22.596244703435563	22.96480980333282	26.27825034223217	28.160695150999455
58-59	19.333303918954435	22.57297803321395	32.85435853953772	25.239359508293894
60-61	22.79056740949052	26.047431512433363	26.437994318040126	24.724006760035987
62-63	19.4746478454481	31.037651068955828	24.20786205957303	25.279839026023037
64-65	19.62592444839967	31.894045171387365	23.240767023659743	25.239263356553227
66-67	19.230238005260016	31.754720492009263	22.50681481168867	26.508226691042047
68-69	19.325595756350708	32.12481637318339	22.612180163317593	25.937407707148306
70-71	19.528204636444276	31.500011986670824	22.60820367751061	26.363579699374295
72-73	20.037824043394075	30.353507115504307	22.280413877894706	27.32825496320691
74-75	20.25749558406389	30.7771088612159	21.808107677879278	27.15728787684093
76	20.082889192760526	30.918320966439655	21.527792427970596	27.470997412829224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	4.0
12	8.0
13	6.0
14	24.0
15	65.5
16	118.0
17	149.0
18	188.5
19	306.0
20	472.5
21	561.0
22	658.5
23	1029.5
24	1648.5
25	1994.0
26	2294.0
27	2904.0
28	3769.0
29	4324.0
30	4736.5
31	5807.0
32	7442.0
33	8419.0
34	9240.0
35	11097.0
36	12861.5
37	13590.0
38	14743.0
39	22068.5
40	31136.0
41	33071.5
42	32112.0
43	30219.0
44	26913.5
45	24987.5
46	24474.0
47	24137.5
48	23290.0
49	21949.5
50	21120.0
51	20558.0
52	18849.0
53	17340.5
54	16979.0
55	16229.5
56	14866.5
57	13504.5
58	12756.0
59	11856.0
60	10651.0
61	9810.0
62	9274.0
63	8683.0
64	7769.5
65	6978.5
66	6196.0
67	5882.0
68	5658.0
69	5296.5
70	4650.5
71	4142.0
72	4193.0
73	3977.0
74	3380.5
75	3051.0
76	2849.5
77	2403.5
78	2020.0
79	1881.0
80	1718.5
81	1391.0
82	1130.5
83	1035.0
84	829.0
85	544.0
86	372.5
87	280.0
88	246.5
89	169.0
90	87.0
91	42.0
92	35.0
93	25.0
94	11.0
95	5.5
96	4.0
97	4.0
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05503482342834351
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001725830699843525
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.8351793329856104E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015436596815267083
52-53	0.030681434663884885
54-55	0.050049090295462215
56-57	0.053213113245175345
58-59	0.038543552296505385
60-61	0.03624244469671402
62-63	0.030969073113858804
64-65	0.018408860798330928
66-67	0.03777651642990826
68-69	0.009587948332464026
70-71	0.014477801982020679
72-73	0.024257509281133988
74-75	0.01553247629859172
76	0.013231368698800356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	521488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.355859683288912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	29.97712474990091	7.600957688669345
2	14.87847867006199	7.545132349177977
3	14.599541382415621	11.10551768198701
4	12.578080401884097	12.757121670211976
5	9.771336308222336	12.388031617475592
6	6.564968751454662	9.987625589223647
7	4.23535634386429	7.517377081462534
8	2.431720759711321	4.932669629774478
9	1.438839820476066	3.283471853524776
>10	3.4474125519567425	13.891467904293053
>50	0.061864961031763574	1.0050910886051898
>100	0.010692709314131967	0.5095172743969658
>500	0.0022912948530282784	0.38402613269828323
>1k	0.0015275299020188525	1.1594374465277972
>5k	0.0	0.0
>10k+	7.637649510094263E-4	5.932554991971361
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	30634	5.874344184334059	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	3788	0.7263829656674746	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	2199	0.4216779676617679	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	805	0.15436596815267084	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	668	0.1280949897217194	RNA PCR Primer, Index 5 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0063280458994262575	0.0
2	0.0	0.0	0.0	0.006903322799374099	0.0
3	0.0	0.0	0.0	0.006903322799374099	0.0
4	0.0	0.0	0.0	0.011697296965606112	0.0
5	0.0	0.0	0.0	0.0147654404319946	0.0
6	0.0	0.0	0.0	0.0147654404319946	0.0
7	0.0	0.0	0.0	0.01514895836529316	0.0
8	0.0	0.0	0.0	0.01514895836529316	0.0
9	0.0	0.0	0.0	0.017833583898383087	0.0
10	0.0	0.0	0.0	0.01917589666492805	0.0
11	0.0	0.0	0.0	0.020518209431473016	0.0
12	0.0	0.0	0.0	0.021477004264719417	0.0
13	0.0	0.0	0.0	0.02205228116466726	0.0
14	0.0	0.0	0.0	0.023969870831160065	0.0
15	0.0	0.0	0.0	0.024545147731107907	0.0
16	0.0	0.0	0.0	0.024545147731107907	0.0
17	0.0	0.0	0.0	0.024545147731107907	0.0
18	0.0	0.0	0.0	0.02531218359770503	0.0
19	0.0	0.0	0.0	0.027805050164145674	0.0
20	0.0	0.0	0.0	0.027996809130794956	0.0
21	0.0	0.0	0.0	0.031448470530482005	0.0
22	0.0	0.0	0.0	0.034324855030221216	0.0
23	0.0	0.0	0.0	0.03662596263001258	0.0
24	0.0	0.0	0.0	0.039502347129751786	0.0
25	0.0	0.0	0.0	0.04161169576289387	0.0
26	0.0	0.0	0.0	0.053500751695149264	0.0
27	0.0	0.0	0.0	0.13653238425428774	0.0
28	0.0	0.0	0.0	0.1829380541834136	0.0
29	0.0	0.0	0.0	0.20748320191452152	0.0
30	0.0	0.0	0.0	0.24430092351118338	0.0
31	0.0	0.0	0.0	0.28725493204062225	0.0
32	0.0	0.0	0.0	0.3305924585033596	0.0
33	0.0	0.0	0.0	0.38447672813180744	0.0
34	0.0	0.0	0.0	0.45830393029178046	0.0
35	0.0	0.0	0.0	0.5833307765471113	0.0
36	0.0	0.0	0.0	0.8341515049243703	0.0
37	0.0	0.0	0.0	1.267335010585095	0.0
38	0.0	0.0	0.0	1.807328260669469	0.0
39	0.0	0.0	0.0	2.3722501764182495	0.0
40	0.0	0.0	0.0	3.043406559690731	0.0
41	0.0	0.0	0.0	4.008337679869911	0.0
42	0.0	0.0	0.0	5.274138618721811	0.0
43	0.0	0.0	0.0	6.465728837480441	0.0
44	0.0	0.0	0.0	7.372748749731538	0.0
45	0.0	0.0	0.0	8.193285368023808	0.0
46	0.0	0.0	0.0	9.004809314883564	0.0
47	0.0	0.0	0.0	9.785651827079434	0.0
48	0.0	0.0	0.0	10.489407234682293	0.0
49	0.0	0.0	0.0	11.143113551989691	0.0
50	0.0	0.0	0.0	11.784163777498236	0.0
51	0.0	0.0	0.0	12.541228177829595	0.0
52	0.0	0.0	0.0	13.541442947872243	0.0
53	0.0	0.0	0.0	14.598418372043076	0.0
54	0.0	0.0	0.0	15.45040346086583	0.0
55	0.0	0.0	0.0	16.1919353848986	0.0
56	0.0	0.0	0.0	16.8845687724358	0.0
57	0.0	0.0	0.0	17.542110269076183	0.0
58	0.0	0.0	0.0	18.10511459515847	0.0
59	0.0	0.0	0.0	18.67463872610683	0.0
60	0.0	0.0	0.0	19.235917221489277	0.0
61	0.0	0.0	0.0	19.827685392568956	0.0
62	0.0	0.0	0.0	20.427699199214555	0.0
63	0.0	0.0	0.0	21.064530727456816	0.0
64	0.0	0.0	0.0	21.698677630165985	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTACG	40	7.464387E-7	52.51223	48
GACGATC	20	0.006946152	52.507195	69
TCTCGTA	4325	0.0	52.34547	38
CTCGTAT	4520	0.0	52.332226	39
CGTATGC	4585	0.0	52.124546	41
TCGTATG	4600	0.0	52.030643	40
TATGCCG	4580	0.0	51.87585	43
ATCTCGT	4360	0.0	51.845013	37
CCGTCTT	4575	0.0	51.727894	47
GCCGTCT	4580	0.0	51.671425	46
ATGCCGT	4580	0.0	51.646652	44
TGCCGTC	4610	0.0	51.401245	45
GTATGCC	4635	0.0	51.335773	42
CGTCTTC	4595	0.0	51.274185	48
GATCTCG	4420	0.0	50.903736	36
CTGCTTG	4585	0.0	50.866177	54
CTTGAAA	4580	0.0	50.39133	57
TGCTTGA	4635	0.0	50.24191	55
GCTTGAA	4635	0.0	50.020054	56
GTCTTCT	4710	0.0	49.952732	49
>>END_MODULE