FastQCFastQC Report
Wed 25 May 2016
SRR1779850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90503
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG150.002218269370.046
TCGGTGG950.066.3157945
CGGTGGT1000.062.99999646
CTCGGTG900.062.22222544
ATCTCGG1300.059.23076642
TCGCCGT900.058.33333652
AGATCTC1700.057.6470640
TGGTCGC850.057.6470649
TCTCGGT1100.057.27272443
ATCTCCG252.379245E-455.99999642
GTGTCGA252.379245E-455.99999636
AGAGGAT252.379245E-455.99999628
GGTGGTC950.055.26315747
GATCTCG1650.055.15151641
GTGTTAG200.006931463352.5000041
ACCGGGT200.006931463352.50000459
ACGTTTG200.006931463352.50000412
CTATCTG200.006931463352.50000454
GTAGATC1600.052.50000438
TCTCGGA200.006931463352.50000443
Produced by FastQC (version 0.11.5)