##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779850_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90503
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.957846701214326	27.0	12.0	34.0	12.0	34.0
2	25.163740428494084	31.0	12.0	34.0	12.0	34.0
3	25.13649271294874	31.0	11.0	34.0	11.0	34.0
4	25.814149807188713	31.0	21.0	34.0	12.0	34.0
5	24.851662375832845	31.0	11.0	34.0	11.0	34.0
6	27.863606731268575	34.0	21.0	38.0	11.0	38.0
7	28.040109167651902	34.0	21.0	38.0	11.0	38.0
8	27.71448460272035	34.0	21.0	38.0	11.0	38.0
9	28.121885462360364	34.0	12.0	38.0	11.0	38.0
10-11	27.060174800835334	31.5	11.5	37.5	11.0	38.0
12-13	27.4311404041855	33.5	11.0	38.0	11.0	38.0
14-15	26.55329657580412	29.5	11.0	37.5	11.0	38.0
16-17	26.827729467531462	30.5	11.0	37.5	11.0	38.0
18-19	27.108007469365656	32.5	11.0	38.0	11.0	38.0
20-21	26.63898434306045	29.0	11.0	37.5	11.0	38.0
22-23	27.103189949504436	32.5	11.0	38.0	11.0	38.0
24-25	27.414605040716882	33.0	11.0	38.0	11.0	38.0
26-27	26.52487762836591	31.0	11.0	38.0	11.0	38.0
28-29	27.03935228666453	32.0	11.0	38.0	11.0	38.0
30-31	27.239030750361863	34.0	11.0	38.0	11.0	38.0
32-33	27.42048329889617	34.0	11.0	38.0	11.0	38.0
34-35	27.503696010077014	34.0	11.0	38.0	11.0	38.0
36-37	27.123272156724084	32.5	11.0	38.0	11.0	38.0
38-39	26.99661889661116	32.5	11.0	38.0	11.0	38.0
40-41	27.028606786515365	32.0	11.0	38.0	11.0	38.0
42-43	27.311934411014	33.5	11.0	38.0	11.0	38.0
44-45	27.40493132824326	34.0	11.0	38.0	11.0	38.0
46-47	27.283250278996277	34.0	11.0	38.0	11.0	38.0
48-49	26.9809619570622	32.5	11.0	38.0	11.0	38.0
50-51	26.537446272499253	29.5	11.0	38.0	11.0	38.0
52-53	27.022264455321924	32.0	11.0	38.0	11.0	38.0
54-55	27.04825254411456	33.0	11.0	38.0	11.0	38.0
56-57	27.07149486757345	33.0	11.0	38.0	11.0	38.0
58-59	27.09233948045921	33.5	11.0	38.0	11.0	38.0
60-61	27.22196501773422	33.5	11.0	38.0	11.0	38.0
62-63	27.054395986873363	33.0	11.0	38.0	11.0	38.0
64-65	27.02665105024143	33.0	11.0	38.0	11.0	38.0
66-67	27.219871164491785	32.5	11.0	38.0	11.0	38.0
68-69	26.82401688341823	31.0	11.0	38.0	11.0	38.0
70-71	26.83917107720186	31.0	11.0	38.0	11.0	38.0
72-73	26.680419433609934	31.0	11.0	38.0	11.0	38.0
74-75	26.932919350739756	31.0	11.0	38.0	11.0	38.0
76	26.006563318343037	28.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	68.0
13	726.0
14	2533.0
15	4221.0
16	5565.0
17	6504.0
18	6446.0
19	5672.0
20	4342.0
21	3067.0
22	2228.0
23	1548.0
24	1263.0
25	1061.0
26	934.0
27	996.0
28	1018.0
29	1185.0
30	1292.0
31	1482.0
32	1965.0
33	2444.0
34	3383.0
35	4920.0
36	8645.0
37	16992.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.292984762936037	20.104305934609904	16.90662187993768	34.69608742251638
2	21.73850590588157	21.26669834149144	32.35030882954156	24.644486923085424
3	13.974122404782163	28.880810580864726	31.971315867982277	25.173751146370837
4	11.153221440173255	29.748185143033933	23.802525883119895	35.29606753367291
5	20.162867529253173	30.711689115277945	30.075246124437864	19.050197231031017
6	21.103634213986584	45.75640048176263	13.297090639882434	19.84287466436835
7	18.917754179696562	36.487397372288584	15.021492425163267	29.573356022851588
8	16.827438370846732	46.62158429188628	13.730235025027348	22.820742312239645
9	19.432012818387754	22.909553013978673	14.775401955909167	42.883032211724405
10-11	18.37393163573682	32.516394937044545	21.681648167689684	27.428025259528955
12-13	17.818746339900336	25.308000839751166	24.431786791598068	32.441466028750426
14-15	25.530233971437255	27.814701251346648	19.08676555896246	27.56829921825364
16-17	27.151033667392245	25.96378020618101	18.541926786957337	28.343259339469412
18-19	20.523416738949077	25.80288724496279	22.669790003480607	31.003906012607523
20-21	25.259383666839774	24.85442471520281	20.435234191131784	29.45095742682563
22-23	27.138121546961326	24.419337016574584	18.927624309392264	29.514917127071822
24-25	19.70497237569061	31.1475138121547	18.733149171270718	30.414364640883978
26-27	23.918676279660783	30.863235822214858	19.87403662882241	25.344051269301954
28-29	20.696151827398545	29.339484516146857	23.096218127572584	26.868145528882014
30-31	22.246346786000387	27.484876108394797	23.379464655672493	26.889312449932323
32-33	18.51761820050164	30.31667458537286	23.113045976376473	28.052661237749028
34-35	18.766505707245386	32.40533044564028	21.677587597653012	27.150576249461324
36-37	23.58015140631044	26.582859037409516	22.24733381223407	27.589655744045977
38-39	24.52581039045709	23.982142955804918	25.04406247755438	26.44798417618361
40-41	20.395569183171737	28.813568685947903	23.05847904754012	27.73238308334024
42-43	16.667034982652318	28.100483967205143	24.69116704603213	30.541314004110404
44-45	16.501104972375693	24.592265193370167	28.1353591160221	30.771270718232046
46-47	18.812291441073125	27.23586219089081	24.85635676559634	29.095489602439724
48-49	19.50487829132736	28.044926687513122	23.777664828790208	28.67253019236931
50-51	24.708150783697327	27.29929668896857	20.256794161358226	27.735758365975876
52-53	15.512506699524264	30.2448296248819	23.422641905593345	30.8200217700005
54-55	17.703447094639017	26.746338256175655	23.043432618941065	32.50678203024427
56-57	19.647602892993497	28.338186298614833	21.207918713291967	30.806292095099703
58-59	18.485407711093675	28.61215784646326	22.063032499751362	30.8394019426917
60-61	15.903364130675039	26.6632774855223	23.18476194686353	34.248596436939124
62-63	18.503784739488367	27.27333001823305	22.244322890767446	31.97856235151113
64-65	18.924847634255908	26.53014990689527	21.615215024947645	32.92978743390117
66-67	16.372898962327746	32.41261561923285	19.206329911261893	32.00815550717751
68-69	17.819298807774327	34.80879086881098	17.938631868556843	29.43327845485785
70-71	16.264744067843427	35.21725919173503	19.851385320847488	28.666611419574046
72-73	17.9206400070719	32.22079801986762	21.149404965800727	28.70915700725975
74-75	17.159779659986853	32.965363301343146	18.97531948749399	30.899537551176014
76	18.755593860841316	32.26483685263146	18.595374636744346	30.384194649782874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	8.0
15	16.0
16	20.0
17	24.0
18	24.0
19	29.5
20	47.0
21	59.0
22	56.5
23	71.0
24	138.0
25	188.0
26	233.0
27	332.0
28	402.0
29	418.0
30	519.0
31	723.0
32	904.0
33	982.0
34	1088.5
35	1384.5
36	1815.0
37	2056.0
38	2321.0
39	2782.0
40	3324.0
41	3878.0
42	4086.0
43	4336.5
44	4821.5
45	5163.5
46	5271.0
47	5386.0
48	5515.5
49	5424.5
50	5319.0
51	5034.0
52	4654.0
53	4363.0
54	4167.0
55	3915.0
56	3479.5
57	3054.5
58	2813.0
59	2572.0
60	2138.5
61	1830.0
62	1714.0
63	1549.0
64	1269.5
65	1064.0
66	876.5
67	780.0
68	686.5
69	577.5
70	505.0
71	448.0
72	414.0
73	352.0
74	271.0
75	218.0
76	204.5
77	186.0
78	163.0
79	145.0
80	141.5
81	114.0
82	73.5
83	57.0
84	52.5
85	44.0
86	32.5
87	25.0
88	25.5
89	23.5
90	15.5
91	11.0
92	12.0
93	8.0
94	5.0
95	6.5
96	7.0
97	9.0
98	9.5
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.002209871495972509
7	0.006629614487917527
8	0.002209871495972509
9	0.008839485983890037
10-11	0.0027623393699656366
12-13	0.0
14-15	5.524678739931273E-4
16-17	0.0
18-19	0.0016574036219793818
20-21	0.0
22-23	0.0033148072439587637
24-25	0.0033148072439587637
26-27	5.524678739931273E-4
28-29	0.0060771466139244
30-31	5.524678739931273E-4
32-33	0.0
34-35	0.004419742991945018
36-37	0.01988884346375258
38-39	0.007182082361910655
40-41	5.524678739931273E-4
42-43	0.0011049357479862546
44-45	0.0033148072439587637
46-47	0.0011049357479862546
48-49	0.0
50-51	0.0027623393699656366
52-53	0.01270676110184193
54-55	0.007182082361910655
56-57	0.009391953857883165
58-59	0.011049357479862547
60-61	0.02099377921173884
62-63	0.008839485983890037
64-65	0.014916632597814438
66-67	0.013259228975835055
68-69	0.0
70-71	5.524678739931273E-4
72-73	0.004419742991945018
74-75	0.007182082361910655
76	0.002209871495972509
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	90503.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.23377125620145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.93457473578259	73.05724672110317
2	4.309090167167671	7.0008728992409095
3	2.5054747752281723	6.105874943372043
4	1.5030128266162488	4.883816006099245
5	0.7861913246915764	3.193264311680276
6	0.45974509990614665	2.2408096969161244
7	0.21627062392034713	1.2297934875087013
8	0.10745521565853726	0.6983193927273129
9	0.07753097838653952	0.5668320387169485
>10	0.09929406003890151	0.9568743577560965
>50	0.0013601926032726236	0.06629614487917528
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006629614487917527	0.0
2	0.0	0.0	0.0	0.006629614487917527	0.0
3	0.0	0.0	0.0	0.006629614487917527	0.0
4	0.0	0.0	0.0	0.008839485983890037	0.0
5	0.0	0.0	0.0	0.01436416472382131	0.0
6	0.0	0.0	0.0	0.01436416472382131	0.0
7	0.0	0.0	0.0	0.01436416472382131	0.0
8	0.0	0.0	0.0	0.01436416472382131	0.0
9	0.0	0.0	0.0	0.01657403621979382	0.0
10	0.0	0.0	0.0	0.017678971967780073	0.0
11	0.0	0.0	0.0	0.017678971967780073	0.0
12	0.0	0.0	0.0	0.017678971967780073	0.0
13	0.0	0.0	0.0	0.017678971967780073	0.0
14	0.0	0.0	0.0	0.027623393699656364	0.0
15	0.0	0.0	0.0	0.027623393699656364	0.0
16	0.0011049357479862546	0.0	0.0	0.027623393699656364	0.0
17	0.0011049357479862546	0.0	0.0	0.027623393699656364	0.0
18	0.0011049357479862546	0.0	0.0	0.03314807243958764	0.0
19	0.0011049357479862546	0.0	0.0	0.03867275117951891	0.0
20	0.0011049357479862546	0.0	0.0	0.03867275117951891	0.0
21	0.0011049357479862546	0.0	0.0	0.05745665889528524	0.0
22	0.0011049357479862546	0.0	0.0	0.10054915306674916	0.0
23	0.0011049357479862546	0.0	0.0	0.10828370330265295	0.0
24	0.0011049357479862546	0.0	0.0	0.1314873540103643	0.0
25	0.0011049357479862546	0.0	0.0	0.13701203275029558	0.0
26	0.0011049357479862546	0.0	0.0	0.13701203275029558	0.0
27	0.0011049357479862546	0.0	0.0	0.15800581196203442	0.0
28	0.0011049357479862546	0.0	0.0	0.18341933416571826	0.0
29	0.002209871495972509	0.0	0.0	0.21988221384926465	0.0
30	0.002209871495972509	0.0	0.0	0.24419080030496226	0.0
31	0.002209871495972509	0.0	0.0	0.25855496502878356	0.0
32	0.002209871495972509	0.0	0.0	0.29501784471233	0.0
33	0.002209871495972509	0.0	0.0	0.32706098140393136	0.0
34	0.002209871495972509	0.0	0.0	0.3867275117951891	0.0
35	0.002209871495972509	0.0	0.0	0.47070262864214446	0.0
36	0.002209871495972509	0.0	0.0	0.612134404384385	0.0
37	0.002209871495972509	0.0	0.0	0.9038374418527563	0.0
38	0.002209871495972509	0.0	0.0	1.1867009933372374	0.0
39	0.002209871495972509	0.0	0.0	1.4960830027733887	0.0
40	0.002209871495972509	0.0	0.0	1.8629216711048253	0.0
41	0.002209871495972509	0.0	0.0	2.2496491829000145	0.0
42	0.002209871495972509	0.0	0.0	2.7391357192579253	0.0
43	0.002209871495972509	0.0	0.0	3.284973978763135	0.0
44	0.002209871495972509	0.0	0.0	3.6440780968586677	0.0
45	0.002209871495972509	0.0	0.0	4.135774504712551	0.0
46	0.002209871495972509	0.0	0.0	4.5899030971349015	0.0
47	0.002209871495972509	0.0	0.0	5.052871175541142	0.0
48	0.002209871495972509	0.0	0.0	5.405345679148757	0.0
49	0.002209871495972509	0.0	0.0	5.804227484171795	0.0
50	0.002209871495972509	0.0	0.0	6.190954995966985	0.0
51	0.002209871495972509	0.0	0.0	6.5865219937460635	0.0
52	0.002209871495972509	0.0	0.0	7.025181485696606	0.0
53	0.002209871495972509	0.0	0.0	7.440637326939439	0.0
54	0.002209871495972509	0.0	0.0	7.780957537319205	0.0
55	0.002209871495972509	0.0	0.0	8.187573892578147	0.0
56	0.002209871495972509	0.0	0.0	8.577616211617295	0.0
57	0.002209871495972509	0.0	0.0	8.890313028297404	0.0
58	0.002209871495972509	0.0	0.0	9.226213495685226	0.0
59	0.002209871495972509	0.0	0.0	9.530070826381445	0.0
60	0.002209871495972509	0.0	0.0	9.910168723688718	0.0
61	0.002209871495972509	0.0	0.0	10.25490867706043	0.0
62	0.002209871495972509	0.0	0.0	10.611802923659988	0.0
63	0.002209871495972509	0.0	0.0	10.913450382860237	0.0
64	0.002209871495972509	0.0	0.0	11.173110283637007	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGG	15	0.0022182693	70.0	46
TCGGTGG	95	0.0	66.31579	45
CGGTGGT	100	0.0	62.999996	46
CTCGGTG	90	0.0	62.222225	44
ATCTCGG	130	0.0	59.230766	42
TCGCCGT	90	0.0	58.333336	52
AGATCTC	170	0.0	57.64706	40
TGGTCGC	85	0.0	57.64706	49
TCTCGGT	110	0.0	57.272724	43
ATCTCCG	25	2.379245E-4	55.999996	42
GTGTCGA	25	2.379245E-4	55.999996	36
AGAGGAT	25	2.379245E-4	55.999996	28
GGTGGTC	95	0.0	55.263157	47
GATCTCG	165	0.0	55.151516	41
GTGTTAG	20	0.0069314633	52.500004	1
ACCGGGT	20	0.0069314633	52.500004	59
ACGTTTG	20	0.0069314633	52.500004	12
CTATCTG	20	0.0069314633	52.500004	54
GTAGATC	160	0.0	52.500004	38
TCTCGGA	20	0.0069314633	52.500004	43
>>END_MODULE