FastQCFastQC Report
Wed 25 May 2016
SRR1779850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90503
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3042733.61988000397777No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG36224.0020772792062145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC21982.4286487740737877No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT7190.794448802802117RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6640.733677336662873No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3140.34694982486768394No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT2890.31932643116802756No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2830.31269681668011007No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT2490.2751290012485774No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2060.22761676408516845RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1650.182314398417732No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1650.182314398417732No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1610.17789465542578697No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1320.14585151873418561No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1310.14474658298619936No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1150.1270676110184193No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT1100.121542932278488No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT920.10165408881473544RNA PCR Primer, Index 48 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG910.10054915306674918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTTA150.00221833969.9994428
CTCGTCT252.8200193E-669.9994439
ACCGCTC150.00221833969.9994433
CGTTGGT150.00221833969.9994421
GACCGCT150.00221833969.9994432
TTCCGTT150.00221833969.9994418
ACCTCGC150.00221833969.9994437
TAGTTCG150.00221833969.9994454
TGACCGC150.00221833969.9994431
CGCTCCA150.00221833969.9994434
TTGACCG150.00221833969.9994430
AGTTCGC150.00221833969.9994455
ACTAAAG150.00221833969.999443
ACACTTA150.00221833969.999446
GACTGGT150.00221833969.9994465
TAAGCAA150.00221833969.9994423
CTGGCTA150.00221833969.9994414
ACTGGTG150.00221833969.9994466
GTCAAGC401.0524673E-861.24951633
GCTCGTA401.0524673E-861.24951638
Produced by FastQC (version 0.11.5)