##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779850_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90503
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.5641138967769	34.0	34.0	34.0	34.0	34.0
2	33.64635426449952	34.0	34.0	34.0	34.0	34.0
3	33.79025004695977	34.0	34.0	34.0	34.0	34.0
4	33.6009856026872	34.0	34.0	34.0	34.0	34.0
5	33.64458636730274	34.0	34.0	34.0	34.0	34.0
6	37.37724716307747	38.0	38.0	38.0	37.0	38.0
7	37.40763289614709	38.0	38.0	38.0	37.0	38.0
8	37.324596974685925	38.0	38.0	38.0	37.0	38.0
9	37.259350518767334	38.0	38.0	38.0	37.0	38.0
10-11	37.184165165795605	38.0	38.0	38.0	36.5	38.0
12-13	37.34009369855143	38.0	38.0	38.0	36.5	38.0
14-15	37.35224246710054	38.0	38.0	38.0	37.0	38.0
16-17	37.16006651713203	38.0	38.0	38.0	36.0	38.0
18-19	36.99795034418749	38.0	38.0	38.0	35.5	38.0
20-21	37.104543495795724	38.0	38.0	38.0	35.5	38.0
22-23	37.13357015789532	38.0	38.0	38.0	36.0	38.0
24-25	37.256483210501315	38.0	38.0	38.0	36.0	38.0
26-27	37.10662629968067	38.0	38.0	38.0	36.0	38.0
28-29	36.63063102880568	38.0	37.0	38.0	34.0	38.0
30-31	36.94964807796427	38.0	37.0	38.0	35.5	38.0
32-33	36.80437665049777	38.0	37.0	38.0	34.5	38.0
34-35	36.69271736848502	38.0	37.0	38.0	34.5	38.0
36-37	36.531534866247526	38.0	37.0	38.0	34.0	38.0
38-39	35.26143884733103	38.0	36.0	38.0	29.0	38.0
40-41	36.363678552092196	38.0	37.0	38.0	34.0	38.0
42-43	37.08883683413809	38.0	37.5	38.0	36.0	38.0
44-45	36.86415367446383	38.0	37.5	38.0	35.0	38.0
46-47	37.01579505651746	38.0	38.0	38.0	36.0	38.0
48-49	36.596748174093676	38.0	37.0	38.0	34.0	38.0
50-51	36.67240864943703	38.0	37.0	38.0	34.0	38.0
52-53	36.59153287736318	38.0	37.0	38.0	34.0	38.0
54-55	36.61771432991172	38.0	37.0	38.0	34.0	38.0
56-57	36.477763168071775	38.0	37.0	38.0	34.0	38.0
58-59	36.480818315414965	38.0	37.0	38.0	34.0	38.0
60-61	36.05386561771433	38.0	37.0	38.0	33.0	38.0
62-63	35.99923206965515	38.0	37.0	38.0	33.0	38.0
64-65	36.481879053733024	38.0	37.0	38.0	34.0	38.0
66-67	36.10349933151387	38.0	37.0	38.0	32.5	38.0
68-69	35.995232202247436	38.0	37.0	38.0	32.0	38.0
70-71	33.24153895450979	37.5	31.0	38.0	20.5	38.0
72-73	28.44372009767632	35.0	20.5	38.0	11.0	38.0
74-75	27.71800934775643	34.0	19.0	38.0	11.0	38.0
76	27.705943449388418	34.0	19.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	3.0
23	7.0
24	31.0
25	66.0
26	132.0
27	245.0
28	419.0
29	698.0
30	1211.0
31	1834.0
32	2860.0
33	4784.0
34	8363.0
35	18864.0
36	19590.0
37	31394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.397384739524036	11.40832771446573	12.178757364401067	55.01553018160917
2	10.556556136260676	10.523408063821089	59.57703059567086	19.343005204247373
3	10.370926930598985	12.890180436007645	59.170414240411915	17.568478392981447
4	7.474890335127013	46.596245428328345	28.26204656199242	17.666817674552224
5	7.56991480945383	24.883153044650452	59.14942046120019	8.397511684695536
6	16.15416063555904	59.9118261273107	14.571892644442725	9.362120592687535
7	14.185165132647537	22.899793377015126	15.264687358430107	47.65035413190723
8	12.789631282940897	59.49526535031988	11.15432637592124	16.560776990817985
9	12.789631282940897	14.566367965702796	11.3907826259903	61.253218125366004
10-11	13.43878103488282	36.29327204623051	33.05304796526082	17.214898953625845
12-13	13.711700164635426	13.981304487144072	37.01313768604356	35.29385766217694
14-15	13.101223163873021	12.753720871131344	16.740881517739744	57.4041744472559
16-17	34.36626410174248	32.75250544180856	12.554279968619825	20.32695048782913
18-19	35.99770173364419	18.039181021623595	13.449830392362683	32.51328685236953
20-21	14.74592002475056	13.585737489364993	13.169176712374176	58.49916577351028
22-23	12.966421002618697	35.25242257162746	13.130503961194655	38.65065246455919
24-25	34.416918963116835	35.134582661156664	12.77319838235619	17.67529999337031
26-27	35.559042241693646	36.84242511297968	14.480735445233861	13.117797200092815
28-29	14.512778581925462	17.74582058053324	33.39723545075854	34.34416538678276
30-31	12.75316840325735	13.451487795984661	59.03726948277958	14.758074317978409
32-33	31.218302155729642	13.817773996442106	20.839640674894756	34.124283172933495
34-35	16.840325735058506	14.411124493110725	35.32313845949858	33.42541131233219
36-37	12.313956443432815	53.85235848535408	16.04421952863441	17.7894655425787
38-39	12.566986729721666	20.209274830668598	34.83862413400661	32.38511430560313
40-41	12.216169629736031	13.590709700230933	37.853441322387106	36.339679347645934
42-43	33.58912737217204	13.211237258639263	35.545979392834454	17.653655976354244
44-45	13.973017469034177	33.70219771720274	38.54347369700452	13.78131111675856
46-47	33.07735655171652	14.443720097676321	19.844093565959138	32.63482978464802
48-49	32.42212965316067	12.614498966885076	15.703346850380651	39.260024529573606
50-51	15.406122223450106	12.850038678306996	37.19471764835893	34.54912144988396
52-53	11.492232961421774	12.942301209679647	57.55124145515232	18.01422437374625
54-55	11.009225020865461	13.19415656557282	40.59506635492839	35.201552058633325
56-57	32.23909306477556	13.046314055616664	20.0445508161201	34.67004206348768
58-59	13.170664308610588	13.888581850337129	54.966287167016695	17.97446667403559
60-61	32.08052875316379	33.401306409363706	20.353791571338572	14.164373266133934
62-63	12.554155614500443	59.04288240495137	13.862179487179487	14.5407824933687
64-65	10.6636681603943	61.69113543560303	13.403212527420308	14.241983876582365
66-67	10.799533554765866	61.490635172402364	12.945513227922605	14.764318044909169
68-69	10.635887972330115	61.70085805371538	13.093468736014499	14.569785237940009
70-71	11.418499671247107	57.54777967478327	13.59158392591733	17.44213672805229
72-73	12.831621234810767	48.110981560173954	15.654235302568978	23.4031619024463
74-75	13.845975334836227	43.88509481501127	16.765017902134996	25.503911948017503
76	13.746767741507723	44.181934713903686	16.204389241275667	25.866908303312925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	5.5
12	11.0
13	6.5
14	9.0
15	9.5
16	9.0
17	15.0
18	25.5
19	37.5
20	44.0
21	49.0
22	60.0
23	103.0
24	151.5
25	168.0
26	216.5
27	294.0
28	308.5
29	294.0
30	366.5
31	539.0
32	690.0
33	741.0
34	839.0
35	1056.5
36	1260.0
37	1344.0
38	1681.5
39	6438.0
40	13408.5
41	14544.0
42	13128.0
43	10695.5
44	6684.0
45	4178.5
46	3252.0
47	3098.5
48	2698.0
49	2380.0
50	2309.0
51	2135.0
52	1891.5
53	1764.5
54	1707.0
55	1730.0
56	1633.0
57	1348.5
58	1184.0
59	1157.5
60	1040.0
61	872.0
62	795.0
63	785.5
64	715.0
65	599.5
66	537.0
67	529.0
68	451.5
69	362.5
70	319.0
71	287.0
72	270.5
73	222.5
74	186.0
75	181.0
76	157.5
77	122.0
78	101.0
79	92.0
80	70.5
81	52.5
82	44.5
83	33.0
84	29.5
85	23.0
86	17.0
87	14.0
88	12.0
89	7.0
90	2.5
91	1.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03756781543153266
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0011049357479862546
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.524678739931273E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01436416472382131
52-53	0.02707092582566324
54-55	0.04695976928941582
56-57	0.049169640785388324
58-59	0.036462879683546404
60-61	0.02872832944764262
62-63	0.02541352220368386
64-65	0.014916632597814438
66-67	0.03480547606156702
68-69	0.00828701810989691
70-71	0.01049688960586942
72-73	0.021546247085731964
74-75	0.0121542932278488
76	0.00994442173187629
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	90503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.596002342463784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.287553648068666	9.533385633625405
2	15.815450643776824	6.5147011701269575
3	11.856223175965665	7.325724009148868
4	8.798283261802576	7.24837850678983
5	6.389484978540773	6.579892379258147
6	4.136266094420601	5.111432770184414
7	2.532188841201717	3.6507077113465853
8	1.5665236051502145	2.581129907295891
9	0.7939914163090129	1.4717744163176911
>10	1.6630901287553648	4.870556777123411
>50	0.06974248927038626	0.9701335867319315
>100	0.06437768240343347	2.5634509353281105
>500	0.01072961373390558	1.52812613946499
>1k	0.01072961373390558	6.430726053280002
>5k	0.0	0.0
>10k+	0.00536480686695279	33.61988000397777
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	30427	33.61988000397777	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	3622	4.0020772792062145	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	2198	2.4286487740737877	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	719	0.794448802802117	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	664	0.733677336662873	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	314	0.34694982486768394	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	289	0.31932643116802756	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	283	0.31269681668011007	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	249	0.2751290012485774	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	206	0.22761676408516845	RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC	165	0.182314398417732	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	165	0.182314398417732	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	161	0.17789465542578697	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	132	0.14585151873418561	No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	131	0.14474658298619936	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	115	0.1270676110184193	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT	110	0.121542932278488	No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT	92	0.10165408881473544	RNA PCR Primer, Index 48 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG	91	0.10054915306674918	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011049357479862547	0.0
2	0.0	0.0	0.0	0.011049357479862547	0.0
3	0.0	0.0	0.0	0.011049357479862547	0.0
4	0.0	0.0	0.0	0.013259228975835055	0.0
5	0.0	0.0	0.0	0.018783907715766327	0.0
6	0.0	0.0	0.0	0.018783907715766327	0.0
7	0.0	0.0	0.0	0.018783907715766327	0.0
8	0.0	0.0	0.0	0.018783907715766327	0.0
9	0.0	0.0	0.0	0.02651845795167011	0.0
10	0.0	0.0	0.0	0.02872832944764262	0.0
11	0.0	0.0	0.0	0.034253008187573895	0.0
12	0.0	0.0	0.0	0.037567815431532654	0.0
13	0.0	0.0	0.0	0.037567815431532654	0.0
14	0.0	0.0	0.0	0.03977768692750516	0.0
15	0.0	0.0	0.0	0.03977768692750516	0.0
16	0.0	0.0	0.0	0.03977768692750516	0.0
17	0.0	0.0	0.0	0.03977768692750516	0.0
18	0.0	0.0	0.0	0.046407301415422694	0.0
19	0.0	0.0	0.0	0.05524678739931273	0.0
20	0.0	0.0	0.0	0.05524678739931273	0.0
21	0.0	0.0	0.0	0.06519120913118902	0.0
22	0.0	0.0	0.0	0.07734550235903782	0.0
23	0.0	0.0	0.0	0.09391953857883165	0.0
24	0.0	0.0	0.0	0.09944421731876292	0.0
25	0.0	0.0	0.0	0.10165408881473542	0.0
26	0.0	0.0	0.0	0.10275902456272168	0.0
27	0.0	0.0	0.0	0.121542932278488	0.0
28	0.0	0.0	0.0	0.15137619747411687	0.0
29	0.0	0.0	0.0	0.1922588201496083	0.0
30	0.0	0.0	0.0	0.21767234235329216	0.0
31	0.0	0.0	0.0	0.23314144282509972	0.0
32	0.0	0.0	0.0	0.2751290012485774	0.0
33	0.0	0.0	0.0	0.3071721379401788	0.0
34	0.0	0.0	0.0	0.37346828281935407	0.0
35	0.0	0.0	0.0	0.4618631426582544	0.0
36	0.0	0.0	0.0	0.6276035048561927	0.0
37	0.0	0.0	0.0	0.9745533297238765	0.0
38	0.0	0.0	0.0	1.2607316884523165	0.0
39	0.0	0.0	0.0	1.5734285051324266	0.0
40	0.0	0.0	0.0	1.9667856314155332	0.0
41	0.0	0.0	0.0	2.3745069224224613	0.0
42	0.0	0.0	0.0	2.8783576235041934	0.0
43	0.0	0.0	0.0	3.436350176237252	0.0
44	0.0	0.0	0.0	3.8175530092925096	0.0
45	0.0	0.0	0.0	4.322508646122228	0.0
46	0.0	0.0	0.0	4.8031556964962485	0.0
47	0.0	0.0	0.0	5.282697811122283	0.0
48	0.0	0.0	0.0	5.6539562224456645	0.0
49	0.0	0.0	0.0	6.078251549672387	0.0
50	0.0	0.0	0.0	6.47271361170348	0.0
51	0.0	0.0	0.0	6.883749709954366	0.0
52	0.0	0.0	0.0	7.31356971592102	0.0
53	0.0	0.0	0.0	7.731235428659823	0.0
54	0.0	0.0	0.0	8.07708031777952	0.0
55	0.0	0.0	0.0	8.483696673038462	0.0
56	0.0	0.0	0.0	8.871529120581638	0.0
57	0.0	0.0	0.0	9.180911130017789	0.0
58	0.0	0.0	0.0	9.511286918665679	0.0
59	0.0	0.0	0.0	9.822878799597804	0.0
60	0.0	0.0	0.0	10.222865540368828	0.0
61	0.0	0.0	0.0	10.570920300984497	0.0
62	0.0	0.0	0.0	10.931129354828016	0.0
63	0.0	0.0	0.0	11.249350850248058	0.0
64	0.0	0.0	0.0	11.532214401732539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTTA	15	0.002218339	69.99944	28
CTCGTCT	25	2.8200193E-6	69.99944	39
ACCGCTC	15	0.002218339	69.99944	33
CGTTGGT	15	0.002218339	69.99944	21
GACCGCT	15	0.002218339	69.99944	32
TTCCGTT	15	0.002218339	69.99944	18
ACCTCGC	15	0.002218339	69.99944	37
TAGTTCG	15	0.002218339	69.99944	54
TGACCGC	15	0.002218339	69.99944	31
CGCTCCA	15	0.002218339	69.99944	34
TTGACCG	15	0.002218339	69.99944	30
AGTTCGC	15	0.002218339	69.99944	55
ACTAAAG	15	0.002218339	69.99944	3
ACACTTA	15	0.002218339	69.99944	6
GACTGGT	15	0.002218339	69.99944	65
TAAGCAA	15	0.002218339	69.99944	23
CTGGCTA	15	0.002218339	69.99944	14
ACTGGTG	15	0.002218339	69.99944	66
GTCAAGC	40	1.0524673E-8	61.249516	33
GCTCGTA	40	1.0524673E-8	61.249516	38
>>END_MODULE