FastQCFastQC Report
Wed 25 May 2016
SRR1779849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187991
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT750.056.00489846
GCCGTAA252.3848431E-456.00489854
CAACGGG252.3911255E-455.975120
AGCGTAA200.00693332752.5185735
GTCCGCA200.00694063252.5045956
ATTGCGA200.00694063252.5045948
TTCGGTA200.00694794352.4906226
GACGAAT407.4597665E-752.4906223
TCGCCGT750.051.33782252
GCGATCT352.0829517E-549.99106628
ACGAGCG352.0829517E-549.99106624
ATCCCGG305.864255E-446.67074642
CGACGAA2050.046.0770622
GACGACC551.3066165E-744.53749523
CGCCGTA901.8189894E-1242.78151753
GGTGGTC1000.042.00367447
GCGTAGA350.001250911440.0141536
ACACGTA350.001254191639.99285510
GACGGTG350.001255834439.98221214
GATCTCG801.4097168E-939.3784441